Sodium-copper-chlorophyllin-B _HIV 抑制剂 - MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemESodium copper chlorophyllin BCat. No.: HY-B2226CAS No.: 28302-36-5分式: CHCuNNaO分量: 738.13作靶点: HIV; Influenza Virus作通路: Anti-infection储存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性数据体外实验 H2O : 10 mg/mL (

2、13.55 mM; Need ultrasonic)DMSO : 6.67 mg/mL (9.04 mM; Need ultrasonic)Mass Solvent1 mg 5 mg 10 mg Concentration制备储备液1 mM 1.3548 mL 6.7739 mL 13.5477 mL5 mM 0.2710 mL 1.3548 mL 2.7095 mL10 mM 0.1355 mL 0.6774 mL 1.3548 mL请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液，并请注意储备液的保存式和期限。体内实验请根据您的实验动物和给药式选择适当的溶解案，配制前请先配制澄清的

3、储备液，再依次添加助溶剂(为保证实验结果的可靠性，体内实验的作液，建议您现现配，当天使；澄清的储备液可以根据储存条件，适当保存；以下溶剂前的百分 指该溶剂在您配制终溶液中的体积占)：1. 请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% salineSolubility: 0.67 mg/mL (0.91 mM); Clear solution2. 请依序添加每种溶剂： 10% DMSO 90% (20% SBE-CD in saline)Solubility: 0.67 mg/mL (0.91 mM); Clear solution3. 请依序添加

4、每种溶剂： 10% DMSO 90% corn oil1/2 Master of Small Molecules 您边的抑制剂师www.MedChemESolubility: 0.67 mg/mL (0.91 mM); Clear solutionBIOLOGICAL ACTIVITY物活性 Sodium copper chlorophyllin B 对流感病毒和 HIV 发挥抗病毒活性，其 IC50s 值都是50 到 100 M。IC50 & Target IC50: 50 to 100 M (Influenza virus), 50 to 100 M (HIV) 1体外研究 Sodium

5、copper chlorophyllin B exhibits influenza virus adsorption at 200 M (P50 and the 50% cytotoxicconcentration (CC50) of Sodium copper chlorophyllin lay from 50 to 100 M and 200 to 400 M, respectively.Sodium copper chlorophyllin B also inhibits HIV adsorption at 2.5 mM (P50 and CC50 of Sodium copperchl

6、orophyllin lay from 50 to 100 M and 200 to 400 M, respectively 1.PROTOCOLCell Assay 1 MT-2 cells are pre-incubated to the bamboo leaf extract solution (up to 10%), Sodium copper chlorophyllin(up to 5 mM) or test reagent-free culture medium for 10 min to assess the inhibitory activity at adsorption.T

7、he pre-incubated cells are washed extensively to be able to eliminate antiviral activity of test reagentsremaining in the culture medium, and then they are exposed to HIV-1LAI (1.310-4 TCID50 per cell). Inaddition to these pre-incubated cells, control wells are prepared to evaluate growth of the cel

8、ls in a virus-freecondition. Then, virus-exposed and mock-exposed cells are cultured in the absence of test reagents for threedays. The total viable cells are counted on day 3 1.MCE has not independently confirmed the accuracy of these methods. They are for reference only.REFERENCES1. Akiko Ito, et al. In Vitro Inhibition of Cytopathic Effect of Influenza Virus and Human Immunodeficiency Virus by Bamboo Leaf ExtractSolution and Sodium Copper Chlorophyllin. Yonago Acta Med. 2016 Mar; 59(1): 61-65.McePdfHeightCaution: Product has not been fully validated for medical ap