pProEX-HTc大肠杆菌表达载体说明

1、ProEX HTc编号名称北京华越洋 VECT-370pProEX HTcpProEXHTc 载体基本信息别名:pProEX HTc, pProEX HTc质粒类型:大肠杆菌蛋白表达表达水平:高克隆方法:多克隆位点，限制性内切酶载体大小:4779bp5 测序引物序列:M13/pUCReverse: AGCGGATAACAATTTCACACAGG(Invitrogen)3 测序引物序列:pTrcHis Reverse: GATTTAATCTGTATCAGG (Invitrogen)载体标签:N-His, N-TEV 蛋白酶切位点载体抗性:氨苄备注:Hosts: E.coli. Related v

2、ectors: pBR322.稳定性:瞬时表达 Transient组成型/诱导型:诱导型病毒/非病毒:非病毒pProEXHTc 载体质粒图谱和多克隆位点信息pProEXHTc 载体简介pProEX HT 系列原核表达系统是用来在大肠杆菌中表达外源蛋白的载体。使用该载体表达的目的蛋白含有 6 个 His 纯化标签。目的基因可以克隆到载体的多克隆位点处，可以选择的载体有 pProEX HTa, b, c.蛋白表达后，His 纯化标签就位于目的蛋白的 N 端，同时 His 纯化标签的使用可以用于蛋白纯化。载体上同时含有 rTEV 蛋白酶识别位点，可以使用 rTEV 蛋白酶进行酶切，从而去除融合的 H

3、is 标签。pProEXHTc 载体序列ORIGIN1 GTTTGACAGC TTATCATCGA CTGCACGGTG CACCAATGCT TCTGGCGTCA GGCAGCCATC61 GGAAGCTGTG GTATGGCTGT GCAGGTCGTA AATCACTGCA TAATTCGTGT CGCTCAAGGC121GCACTCCCGTTCTGGATAATGTTTTTTGCGCCGACATCATAACGGTTCTGGCAAATATTC181TGAAATGAGCTGTTGACAATTAATCATCCGGTCCGTATAATCTGTGGAATTGTGAGCGGA241TAACAATTTC

4、ACACAGGAAACAGACCATGTCGTACTACCATCACCATCACCATCACGATT301ACGATATCCCAACGACCGAAAACCTGTATTTTCAGGGCGCCATGGGGATCCGGAATTCAA361AGGCCTACGTCGACGAGCTCACTAGTCGCGGCCGCTTTCGAATCTAGAGCCTGCAGTCTC421GAGGCATGCGGTACCAAGCTTGGCTGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATA481CAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTA

5、GC541GCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGT601AGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGC661TCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAG721TAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCG781GGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAG

6、AAGGCCATCCTGACGGA841TGGCCTTTTTGCGTTTCTACAAACTCTTTTTGTTTATTTTTCTAAATACATTCAAATATG901TATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT961ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCT1021GTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCA1081CGAGTGGGTTACATCGAACTGGATCT

7、CAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCC1141GAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCC1201CGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTG1261GTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTA1321TGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATC1381GGAGGA

8、CCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTT1441GATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATG1501CCTACAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCT1561TCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGC1621TCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGA

9、GCGTGGGTCT1681CGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTAC1741ACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCC1801TCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGAT1861TTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATG1921ACCAAAATCCCTTAACGTGAGTTTTCGTTC

10、CACTGAGCGTCAGACCCCGTAGAAAAGATC1981AAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAA2041CCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAG2101GTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTA2161GGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTA2221CCAGTGGCTG

11、CTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAG2281TTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTG2341GAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACG2401CTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAG2461CGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGG

12、TTTCGC2521CACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA2581AACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATG2641TTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCT2701GATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAA2761GAGCGCCTGATGCGGTATTTTCTCCTTACGCATC

13、TGTGCGGTATTTCACACCGCATAATT2821TTGTTAAAATTCGCGTTAAATTTTTGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAA2881ATCGGCAAAATCCCTTATAAATCAAAAGAATAGACCGAGATAGGGTTGAGTGTTGTTCCA2941GTTTGGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGAAAAACC3001GTCTATCAGGGCGATGGCCCACTACGTGAACCATCACCCTAATCAAGTTTTTTGGGGTCG3061AGGTGCCGTAAAGC

14、ACTAAATCGGAACCCTAAAGGGAGCCCCCGATTTAGAGCTTGACGG3121GGAAAGCCGGCGAACGTGGCGAGAAAGGAAGGGAAGAAAGCGAAAGGAGCGGGCGCTAGG3181GCGCTGGCAAGTGTAGCGGTCACGCTGCGCGTAACCACCACACCCGCCGCGCTTAATGCG3241CCGCTACAGGGCGCGTCCCATTCGCCATTCAGGCTGCTATGGTGCACTCTCAGTACAATC3301TGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCAATATTGCTACGTGACTGGTA

15、CA3361CTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTG3421ACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCT3481CCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCAGA3541TCAATTCGCGCGCGAAGGCGAAGCGGCATGCATTTACGTTGACACCATCGAATGGTGCAA3601AACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGA

16、GTCAATTCAGGGTGGTGAATGT3661GAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTC3721CCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCGGC3781GATGGCGGAGCTGAATTACATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAGTC3841GTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTCGC3901GGCGATTAAATCTCGCGC

17、CGATCAACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAACG3961AAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAACGCGTCAGTGG4021GCTGATCATTAACTATCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCTGCCTGCAC4081TAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTT4141CTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAGCA42

18、01AATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGCTG4261GCATAAATATCTCACTCGCAATCAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGGAG4321 TGCCATGTCC GGTTTTCAAC AAACCATGCA AATGCTGAAT GAGGGCATCG TTCCCACTGC4381 GATGCTGGTT GCCAACGATC AGATGGCGCT GGGCGCAATG CGCGCCATTA CCGAGTCCGG4441 GCTGCGCGTT GGTGCGGATA TCTCGGT

19、AGT GGGATACGAC GATACCGAAG ACAGCTCATG4501 TTATATCCCG CCGTTAACCA CCATCAAACA GGATTTTCGC CTGCTGGGGC AAACCAGCGT4561 GGACCGCTTG CTGCAACTCT CTCAGGGCCA GGCGGTGAAG GGCAATCAGC TGTTGCCCGT4621 CTCACTGGTG AAAAGAAAAA CCACCCTGGC ACCCAATACG CAAACCGCCT CTCCCCGCGC4681 GTTGGCCGAT TCATTAATGC AGCTGGCACG ACAGGTTTCC CGACT

20、GGAAA GCGGGCAGTG4741 AGCGCAACGC AATTAATGTG AGTTAGCGCG AATTGATCT/其他大肠杆菌表达载体： pBV221pDsRed-Express2 pCold TFpCold IptdTomato pBV220pCold IVpE-SUMOpET-52b(+)pCold-GST pCold III pCold-ProS2pAmCyan pColdS-SUMO pCold IIpBAD102/D-TOPOpBAD202/D-TOPOpACYC184pBAD/Thio-TOPOpBad/Myc-His CpBad/Myc-His BpBad/Myc-

21、His ApBad/His CpBad/His BpBad/His ApBAD-TOPOpET-23b(+)pET-23a(+)pET-23c(+)pET-23(+)pET-12b(+)pET-12c(+)pET-12a(+)pET-11b(+)pET-11a(+)pET-11c(+)pBad24pQE-82LpQE-81LpQE-80LpQE-32pQE-9pQE-16pQE-31pQE-60pQE-70pQE-40pET-51b(+)pET-50b(+)pET-49b(+)pET-48b(+)pET-47b(+)pET-26b(+)pET-32a(+)pET-21b(+)pET-22b(+

22、)pET-14bpET-16bpET-15bpET-19bpET-20b(+)pET-21d(+)pET-21c(+)pET-21b(+)pET-21a(+)pET-24a(+)pET-24d(+)pET-25b(+)pET-27b(+)pET-28a(+)pET-30a(+)pET-42a(+)pET-43.1c(+)pET-43.1b(+)pET-43.1a(+)pET-44a(+)pET-44c(+)pET-46 EK/LICpET-37b(+)pTrcHis2 CpTrcHis2 BpTrcHis2 ApET303/CT-HispET302/NT-HispRSET-CFPpRSET-E

23、mGFPpRSET-BFPpGFPuvpET300/NT-DESTpET301/CT-DESTpGEM-TpBad43pGEX-4T-3pGEX-5X-2pBlueScript SK(+)pG-Tf2pG-KJE8pGro7pET-SUMOpSE380pET-17bpET102/D-TOPOpCDFDuet-1pMAL-p5xpTf16pET-28c(+)pBluescript II SK(+)pET-30b(+)pSUMOpProEX HTcpProEX HTbpProEX HTapKD3pKD13pKD46pTYB1pTYB2pTWIN2pBluescript II KS(-)pTYB12pMAL-p5epACYCDuet-1pEGM-11ZF(+)pEGM-7ZF(+)PinPoint Xa-3PinPoint Xa-2PinPoint Xa-1pSP73