重组小鼠碱性成纤维细胞生长因子作用机制 - Medchemexpress - MCE中国

1、Product Data SheetRecombinant Mouse Basic Fibroblast Growth FactorCat. No.: HY-P7066Synonyms: rMubFGF; HBGF-2; FGF-2; FGF-b; FGF-basicSpecies: MouseSource: E. coliAccession: P15655Gene ID: 14173Molecular Weight: Approximately 16.5 kDaPROPERTIESAA SequenceM P A L P E D G G A A F P P G H F K D P K R L

2、 Y C K N G G F F L R I H P D G R VR L L A S K C V T E E C F F F E R L E S N N Y N T Y R S R K Y S S W Y V A L K R T G Q Y K L G S K T G P G Q K A I L F L P M S A K SD G V R E K S D P H V K L Q L Q A E E R G V V S I K G V C A N R Y L A M K E D GBiological Activity The ED50 is 1.0 106units/mg.Appearan

3、ce Lyophilized powder.Formulation Lyophilized after extensive dialysis against PBS, pH 7.4.Endotoxin Level 1 EU/g, determined by LAL method.Reconsititution Reconstitute the lyophilized recombinant Murine Basic Fibroblast Growth Factor (rMubFGF) to 0.1-1.0 mg/mL usingsterile distilled water or aqueou

4、s buffer containing 0.1 % BSA.Storage & Stability Lyophilized recombinant Murine Basic Fibroblast Growth Factor (rMubFGF) is stored at -20C. After reconstitution, itis stable at 4C for 1 week or -20C for longer. It is recommended to freeze aliquots at -20C or -80C for extended storage.Shipping Room

5、temperature in continental US; may vary elsewhere.DESCRIPTIONBackground Basic fibroblast growth factor (bFGF) is a heparin-binding mitogen for mesoderm-derived cells, also acts as amitogen, differentiation inducing and maintenance factor for many neuroectodermal cells including glial cells,neurons,

6、paraneurons, and their tumor counterparts. The molecule is expressed in several types of neuroectodermalcells. bFGF occurs in many neuronal target tissues, and can prevent ontogenetic as well as lesion-induced neuronPage 1 of 2 www.MedChemEdeath1. In vitro, basic fibroblast growth factor induces an

7、angiogenic phenotype in endothelial cells, which includeschemotaxis, mitogenesis, protease production, beta-integrin expression, and tube formation in three-dimensionalgels. It acts by binding to specific tyrosine kinase receptors and to cell-associated heparan sulfate proteoglycans2.REFERENCES1. We

8、stermann R, et al. Basic fibroblast growth factor (bFGF), a multifunctional growth factor for neuroectodermal cells. J Cell Sci Suppl. 1990;13:97-117.2. Rusnati M, et al. Interaction of angiogenic basic fibroblast growth factor with endothelial cell heparan sulfate proteoglycans. Biological implications inneovascularization. Int J Clin Lab Res. 1996;26(1):15-23.McePdfHeightCaution: Product has not been fully validated for medical applications. For research us