生物化学与分子生物学PPT图片素材-Science_Slide

1、2021/6/16生物化学与分子生物学PPT素材可根据需要选择取舍2021/6/162Metabolism of 5-fluorouracil (5-FU)5-FUCarac, Efudex5-Fluorouridine5-FUMP5-FdUMPUridine phosphorylase5-FluorodeoxyuridineThymidine phosphorylaseOrotatephosphoribosyltransferaseThymidine kinase5-FUTP5-FdUTPRNADNA5-nucleotidase5-nucleotidaseUridine kinaseATPA

2、DPATPADPPRPP2021/6/163Western Blotting1. Resolve protein samples on SDS-PAGE6. Incubate the blot with chemiluminescent HRP substrate and expose to film 2. Electrophoretically transfer fractionated proteins from gel onto PVDF membrane3. Block the membrane with neutral protein (BSA or milk casein)4. I

3、ncubate the membrane with primary antibody specific to target protein5. Incubate the membrane with HRP-labeled secondary antibody specific to primary antibody2021/6/1642021/6/1652021/6/166CholesterolSterol ToolkitPregnenolonProgesteronAldosteronCortisolCorticosteroneAndrostendioneTestosteronEstriol1

4、7 -Estradiol1, 25-DihydroxycholecalciferolCholic acid2021/6/167Covalent modification of proteinsPoly(ADP ribosyl) Polymerase (PARP)Poly(ADP ribosyl)polymerase(PARP)NADNicotinamidePoly(ADP ribosyl)polymerase(PARP)Poly(ADP ribosyl)chain elongationand branchingNicotinamideNADNADDNA polymerase DNA polym

5、erase DNA polymerase 2021/6/168OligosacharyltransferaseN-glycosylation pathway in ERMann-P-DolicholtransferasesFlippaseDolicholPDolicholPPDolicholPPDolicholPPDolicholPPGlcNAc-PtransferaseGlcNActransferaseDolicholPDolicholPPDolicholPPGDP-Mannosyl transferasesGlc-P-DolicholtransferasesDolicholPDolicho

6、l-pyrophofphataseCytosolER40S60S2021/6/169UTPCTPTMPPyrimidine Nucleotide InterconversionsUMPATPADPH2OUDPNucleosidemonophosphatekinaseATPADPNucleosidediphosphatekinaseATPADPCTPsynthaseRibonucleotidereductasedeoxyUDPNucleosidediphosphatephosphohydrolasedeoxyUMPThymidylatesynthaseN5,N10-MethyleneTHFDHF

7、TTPNucleosidemonophosphatekinaseNucleosidediphosphatekinase2021/6/1610Myo-Inositol-3-phosphateIns(3)P1Biosynthesis and metabolism of phosphoinositidesGlucose 6-pgosphateIns(3)P1synthaseIns(3)P1phosphataseMyo-InositolInsPtdInssynthasePtdInsMyo-inositolkinasePtdIns-4-PPtdIns4-kinasePtdIns5-kinasePtdIn

8、s-4,5-P2PI3KPtdIns-3,4,5-P3PLCPLCPI3KPtdIns-3,4-P2Ins-1,3,4-P3Ins-1,3,4,5-P4Ins-1,4,5-P3PLCPTENPTENIns-1,4-P3PTENPTEN2021/6/1611Fatty acidsynthase -ketoacylACP-synthaseFatty acids synthesisAcetyl CoAcarboxylaseATPADP + PiAcetyl CoAMalonyl CoACitrateOxaloacetateATPADP + PiCitrate lyaseAcetyltransacyl

9、aseMalonyltransacylasePantetheine -ketoacylACP-reductasePantetheinePantetheine -hydroxyacylACP-dehydratasePantetheineEnoyl-ACPreductasePantetheineNADPHNADPNADPHNADP2021/6/1612Cholesterol de novo synthesis IIsoprenyl formationAcetoacetyl-CoAThiolaseHMG-CoAsynthaseMevalonate3-Hydroxy-3-methylglutaryl-

10、CoAMevalonatekinasePhosphomevalonatekinaseMevalonate-5-pyrophosphodecarboxylase5-Phosphomevalonate5-Pyrophosphomevalonate3-Isopentenylpyrophosphate3, 3-DimethylalylpyrophosphateIsomeraseHMG-CoAreductase2 NADPH2 NADPATPADPADPATPADPATP2021/6/1613CholesterolBiosynthesis of bile acids7 -Hydroxycholester

11、olSterol 7 hydroxylaseCYP7A17 -Hydroxy-4-cholesten-3-one3 -Hydroxystroid dehydrogenaseHSD3B77 , 12 -Dihydroxy-4-cholesten-3-oneSterol-12 -hydroxylaseCYP8B15 -Cholestan-3 , 7 , 12 -TriolOxosteroid-5 -reductaseAKR1D1Sterol 27-hydroxylaseCYP27A13 , 7 , 12 -Trihydroxy-5 -cholestanoic acidCholic acidAKR1

12、D1AKR1C45 -Cholestan-3 , 7 -diol12 -Deoxycholic acid3 -Hydroxy-steroid dehydrogenaseAKR1C42021/6/1614Biosynthesis of RNARNAPolymerase2021/6/1615Biosynthesis of DNADNAPolymerase2021/6/1616-oxidationMitochondrial matrixIntermembranespacePalmitoyl carnitinePalmitoyl carnitinePalmitoyl CoAPalmitoyl CoAT

13、rans 2 enolopalmitoyl CoA3-Hydroxypalmitoyl CoA3-Ketopalmitoyl CoAMyristoyl CoAAcetyl-CoAPalmitoyl CoAPalmitatePalmitoyl CoAligaseATPAMPCarnitineacyltransferase ICarnitineCarnitineacyltransferase IIAcyl CoAdehydrogenaseEnoyl CoAhydratase3-Hydroxyacyl CoAdehydrogenaseCitric AcidCycleFADFADH2NADNADHCy

14、toplasmH2O2021/6/1617Myo-Inositol-3-phosphateIns(3)P1Phospho-myo-inositol Toolkit 2Myo-Inositol-3,4-diphosphateIns(3,4)P2Myo-Inositol-3,6-diphosphateIns(3,6)P2Myo-Inositol-3,4,6-triphosphateIns(3,4,6)P3Myo-Inositol-1,3,4-triphosphateIns(1,3,4,)P3Myo-Inositol-3,4,5,6-tetraphosphateIns(3,4,5,6)P4Myo-I

15、nositol-1,4,5-triphosphateIns(1,4,5)P3Myo-Inositol-1,3,4,6-tetraphosphateIns(1,3,4,6)P4Myo-Inositol-1,3,4,5-tetraphosphateIns(1,3,4,5)P4Myo-Inositol-1,4,5,6-tetraphosphateIns(1,4,5,6)P4Myo-Inositol-1,2,3,4,6-pentaphosphateIns(1,2,3,4,6)P5Myo-Inositol-1,3,4,5,6-pentaphosphateIns(1,3,4,5,6)P5Myo-Inosi

16、tol-1,2,3,4,5-pentaphosphateIns(1,2,3,4,5)P5Myo-Inositol-1,2,4,5,6-pentaphosphateIns(1,2,4,5,6)P5Myo-Inositol-1,2,3,4,5,6-hexaphosphateIns(1,2,3,4,5,6)P6 (Phytic acid)2021/6/1618Myo-Inositol-3-phosphateIns(3)P1Phospho-myo-inositol ToolkitMyo-Inositol-3,4-diphosphateIns(3,4)P2Myo-Inositol-3,6-diphosp

17、hateIns(3,6)P2Myo-Inositol-3,4,6-triphosphateIns(3,4,6)P3Myo-Inositol-1,3,4-triphosphateIns(1,3,4,)P3Myo-Inositol-3,4,5,6-tetraphosphateIns(3,4,5,6)P4Myo-Inositol-1,4,5-triphosphateIns(1,4,5)P3Myo-Inositol-1,3,4,6-tetraphosphateIns(1,3,4,6)P4Myo-Inositol-1,3,4,5-tetraphosphateIns(1,3,4,5)P4Myo-Inosi

18、tol-1,4,5,6-tetraphosphateIns(1,4,5,6)P4Myo-Inositol-1,2,3,4,6-pentaphosphateIns(1,2,3,4,6)P5Myo-Inositol-1,3,4,5,6-pentaphosphateIns(1,3,4,5,6)P5Myo-Inositol-1,2,3,4,5-pentaphosphateIns(1,2,3,4,5)P5Myo-Inositol-1,2,4,5,6-pentaphosphateIns(1,2,4,5,6)P5Myo-Inositol-1,2,3,4,5,6-hexaphosphateIns(1,2,3,

19、4,5,6)P6 (Phytic acid)2021/6/1619GlycogenGlycogenin2021/6/16208-oxoguanineA common DNA lesions Toolkit IO6-methylguanineUracilThymine glycol1, N6-ethenoadenine3-methyladenineCyclobutane pyrimidine dimersPhotoadduct2021/6/1621RiboseGlucuronateArabinoseFructoseGlucoseGalactoseMannoseFucoseSialic AcidI

20、nositolD-AbequoseL-RhamnoseA carbohydrate toolkit2021/6/1622PGH2TXA2TXB2Bioactive lipids - ProstanoidsPGD2PGJ2PGE2PGA2PGB2PGF2PGF-MPGI26-keto-PGF1 2021/6/1623Bioactive lipids - Leukotrienes5-HETELTA4LTB4LTC4LTD4LTE420-OH- LTE4LXA4LTB4LTF4LXALXB2021/6/1624Bioactive lipidsArachidonateCeramideSphingosi

21、ne-1-phosphatePAFPhosphatidic acidAnandamide 2-Arachidonoylglycerol 2021/6/1625 qGTP NO mediated attenuation of adrenergic Gq signaling in VSMC qGDP PLC qGTPCasGCcGMPpGCANPSERCABKCaPKGPKCMLCKmyosinmyosinmyosinPPCaM MLCKCaCaCaCaCaCaCaCaCaCaCaCaCaCaCaCaK+CaCaCaCaCaCaCaCaCaCaCaCaCaCaCaCaCaCaM Ca PA1,4,

22、5-InsP2021/6/1626 SGTP RGS function and regulation SGDP ACPKAcAMP cAMPcAMPcAMPcAMPcAMPcAMPAC SGTPLigandLigandRGSRGSRGS14-3-3RGSP14-3-3PcAMPcAMPcAMPTNF 2021/6/1627Modular Structure of Cytokine ReceptorsIFN R2515 aaIFN R1 530 aaIL-6R 449 aaIL-11R 400 aaIL-10R1 557 aaIL-10R2325 aaSTATbindingIL-13R 1 42

23、7 aaIL-4R 825 aaFNIIIFNIIICCCCWS-WS FNIIIFNIIIFNIIIFNIIIFNIIIFNIIIFNIIIFNIIIFNIIIFNIIISOCSgp130 896 aaSTATbindingTyk2Jak1INF R1 489 aaSTATbindingFNIIIFNIIIJak1IL-22R1 574 aaSTATbindingFNIIIFNIIIJak1Jak1INF R2 332 aaFNIIIFNIIIJak2STATbindingTyk2Jak1Jak2Jak1IL-13R 2 380 aaFNIIIFNIIICCCCWS-WS FNIIIFNII

24、ICCCCWS-WS FNIIIFNIIIFNIIISTATbindingSTATbindingIgIgIgIgOSM -R952 aaJak3FNIIIFNIIIFNIIIFNIIISTATbindingIgFNIIIFNIIICCCCWS-WS FNIIIFNIIICCCCWS-WS FNIIIFNIIICCCCWS-WS LIF-R 1053 aaJak3FNIIIFNIIIFNIIISTATbindingIgFNIIIWS-WS FNIIIFNIIICCCCWS-WS STATbindingSTATbindingSHPFNIIICCCCSTATbindingSTATbindingTyk

25、2FNIIIFNIIICCCCWS-WS IgSOCSSHPFNIIIFNIIICCCCWS-WS IgJak3 c 349 aaIRSFNIIIFNIIICCCCWS-WS IgJak3FNIIIFNIIIFNIIIFNIIIFNIIICCCCWS-WS IL-12 662 aaFNIIIWS-WS SOCSIRSSTATbindingIgTyk2Fibronectintype III domainIg - likeIRS-bindingFibronectintype III domainwith cysteinesSOCS-bindingSTAT-bindingJAK/TYK-bindin

26、gFibronectintype III domainwith WSXWSSHP-bindingSHP2021/6/1628Adaptor proteins - selected (human)SAMdomainSrc-homology 3 domainSH3SH2Src-homology 2 domainGRB2PTBdomainPTBCysteine-richregion 1Calponinhomology domainC-1CalponinSproutySprouty domain1217SH2SH3SH31276SH2SH3SLAP1304SH2SH3SH31330SH2SH3SH3C

27、rkGADS (GRID)1377SH2SH3SH3SH3Nck11456SH2SproutyBLNKRho-GEFRho-GEF homologydomainVav-11533SH2SAMSLP-761584SH2PTBSHC-11632SH2PHAPS1845SH2SH3SH3PHC-1Rho-GEFCalponinSAMPHPlekstrin homologydomain2021/6/1629Protein separation using SDS-PAGE(Laemmli system)StackinggelResolvinggel1. Apply protein/dye sample

28、s into polyacrylamide gel wells2. Run the electrophoresis until dye reaches the end of the gel3. Remove the gel from the apparatus and stain for proteins2021/6/1630A Column Chromatography Toolkit2021/6/1631Embryonic stem cellsEgg donorSperm donorEgg fertilizationCulture fertilized cells forseveral c

29、ell divisionsDissociate cell clusterfor subculturing andfurther utilizationFreeze in N2 forlong-term storageUse for In VitrofertilizationPotential use fortherapeutic cloning2021/6/1632Tet rAmp rOripBR3224361bpOripBluescript IIAmp rLacIMCSLacZf1 OriOripUC18Amp rMCSLacZCommon Cloning VectorsOripCR2.13

30、929bpAmp rKanLacZf1 OriM13 (-)APCRAT7M13 (+)OripGEM 11Zf3221bpAmp rLacZf1 OriT7 SfiI-SacI-EcoRI-SalI-Xho-BamHI-ApaI-XbaI-NotI-SphI-NsiI-HindIII SP6OripGEM 9Zf2912bpAmp rLacZf1 OriSP6 NsiI-HindIII-XbaI-EcoRI-SalI-SacI T72021/6/1633Metabolism of capecitabine5-FU5-Fluorouridine5-FUMP5-FdUMPUridine phos

31、phorylase5-FluorodeoxyuridineThymidine phosphorylaseOrotatephosphoribosyltransferaseThymidine kinase5-FUTP5-FdUTPRNADNA5-nucleotidase5-nucleotidaseUridine kinaseATPADPATPADPPRPPCapecitabineXeloda(prodrug)Carboxyl-esterase5-Fluoro-5-deoxycytidineCytidinedeaminaseThymidine phosphorylase5-Fluoro-5-deox

32、yuridinePharmacologicallyactive compound2021/6/1634PertussistoxinNADADPCholeratoxin ACATPcAMPAC SGTPLigandPharmacological modulation of cAMPLigandLigandLigand iGDP SGDP iGTP iGTPNADADPForskolinRolipramAMPPDE SGTP2021/6/1635Mechanism ofPenicillin(inhibition of crosslinkingpeptidoglycan)Tetra-peptideP

33、enta-glycineGlycopeptidetranspeptidasePenicillin2021/6/163640SInhibition of translation byRapamycin(Sirolimus)Growth Factor ReceptorsIP3KPDKPKBmTOR40S30SeIF4GeIF4EPABPeIF4A4E-BP1eIF4EPP4E-BP1PPPPPS6KPPPPPRibosomalproteinseEF2eEF2PP40S30SeEF2kinase CaMeIF2RapamycinFKBP-122021/6/1637TMPMechanism of 5-

34、fluorouracil (5-FU)deoxyUMPThymidylatesynthaseTTP5-FUCarac, EfudexDNA synthesis5-Fluorouridine5-FUMP5-FdUMPUridine phosphorylaseUridine kinase5-FluorodeoxyuridineThymidine phosphorylaseOrotatephosphoribosyltransferaseThymidine kinaseN5,N10-methylene-THFDHF2021/6/1638The Life cycle of Plasmodium spp.

35、Infected mosquito bitereleases sporozoitesinto human bloodBloodvesselEndotheliumHepatocytesKupffer cellsMerozoiteSporozoiteTrophozoiteSchizontMacrogametocyteMicrogametocyte1234Mosquito bitetakes up macrogametocytesand microgametocytesOocystGametogenesisMosquito gut2021/6/1639The Life cycle of HeartwormDirofilaria immitisMicrofilariaeLarvae14 daysMicrofilariaeLarvae14 daysLarvaeAdult worm6monthsLarvaeAdult worm8monthsMicrofilariaeMicrofilariae2021/6/1640Virus envelopes III2021/6/1641HIV envelope2021/6/1642Bacteria & Bacteriophage ToolkitBacilliCoc