ABD活性因子与抗氧化研究

1、美源康富A.B.D活性因子与抗氧化抗衰老研究美源康富ABD活性因子制造内源性抗氧化剂美源康富ABD活性因子激活全身细胞合成谷胱甘肽的能力，谷胱甘肽是每个细胞都能产生的抗氧化剂，叫做内源性的抗氧剂。ABD活性因子是激活了人体的自我抗氧化机制。美源康富ABD活性因子制造“抗氧化剂之王”谷胱甘肽谷胱甘肽被称为“抗氧化剂之王”，科学已知的4000多种外源性抗氧化剂都要通过谷胱甘肽才能更好的发挥作用，需要谷胱甘肽回收利用。美源康富ABD活性因子预防衰老引起衰老，以及老化性疾病的一个首要原因是氧化自由基。ABD活性因子激活人体所有细胞的抗氧化能力，提升细胞自愈机制，赋予每个器官和组织自我抗氧化、抗衰老的活

2、力。对很多老化性疾病的治疗和预防，都有深远的意义。1. The Influence of Dietary Whey Protein on Tissue Glutathione and the Diseases of AgingGustavo Bounous1,2, Francine Gervais1,3, Victor Amer1,3, Gerald Batist3, and Phil Gold1,3The Montreal General Hospital Research Institute1 and McGill University, Departments of Surgery2,

3、and Medicine3CLIN INVEST MED, 12: 343-349, 1989Abstract: This study compared the effects of a whey-rich diet (20 g / 100 g diet), with that of Purina mouse chow or casein-rich diet (20 g / 100 g diet), on the liver and heart glutathione content and on the survival of old male C57BL / 6 NIA mice.

4、0; The study was performed during a limited observation period of 6.3 months.  In mice fed the whey protein-rich diet between 17 months and 20 months of age, the heart tissue and liver tissue glutathione content were enhanced above the corresponding values of the casein diet-fed and Purina-fed

5、mice.  Mice fed the whey protein diet at the onset of senescence, exhibited increased longevity as compared to mice fed Purina mouse chow over the 6.3 month observation period extending from the age of 21 months (corresponding to a human age of 55 years) to 26-27 months of age (corresponding to

6、 a human age of 80 years), during which time 55% mortality was observed.  The corresponding mean survival time of mice fed the defined casein diet is almost identical to that of Purina-fed controls.  Body weight curves were similar in all three dietary groups.  Hence, a whey protein d

7、iet appears to enhance the liver and heart glutathione concentration in aging mice and to increase longevity over a 6.3 month observation period.研究主题美源康富ABD活性因子对组织谷胱甘肽和老化疾病的影响研究目的比较含有ABD活性因子饲料（20g/100g）、普通小鼠饲料和含有酪蛋白饲料（20g/100g）对老年小鼠肝脏和心脏谷胱甘肽浓度，以及存活率的影响。研究内容7个月大的C57BL/6NIA小鼠分为三组，分别喂食以上三种不同的饲料，观察周期为6.

8、3个月。研究结果小鼠20个月大的时候，检测其肝脏和心脏中的谷胱甘肽浓度，ABD活性因子饲料组明显高于其他两组。在6.3个月的观察期间，ABD活性因子饲料组的小鼠的存活时间也长于其他两组，从21个月（相当于人的55 岁）到26-27个月（相当于人的80岁）的存活率为55%。而酪蛋白饲料组合普通饲料组的存活时间无差别。三组的小鼠体重无差别。肝脏谷胱甘肽水平ABD活性因子Purina鼠粮酪蛋白图1 小鼠肝脏中谷胱甘肽的含量。使用Purina鼠粮的小鼠，在年龄10周、17个月和20个月时，肝脏中的谷胱甘肽水平无明显差别。小鼠从17个月大时开始分别服用ABD活性因子和酪蛋白，到20个月大时，发现ABD活

9、性因子组的肝脏谷胱甘肽水平明显高于酪蛋白组和Purina组；酪蛋白组合Purina组之间无明显差异。心脏谷胱甘肽水平Purina鼠粮酪蛋白ABD活性因子图2 小鼠心脏中谷胱甘肽的含量。使用Purina鼠粮的小鼠，在年龄10周、17个月和20个月时，心脏中的谷胱甘肽水平无明显差别。小鼠从17周大时开始分别服用ABD活性因子和酪蛋白，到20周大时，发现ABD活性因子组的心脏谷胱甘肽水平明显高于酪蛋白组和Purina组；酪蛋白组合Purina组之间无明显差异。小鼠粗活率生命时间（天）酪蛋白Purina鼠粮ABD Bioactive Cytokines图2 使用不同饲料的小鼠在不同年龄的存活率。ABD

10、活性因子组的寿命超过60天的小鼠的比例明显高于Purina鼠粮组和酪蛋白组。与人类寿命比较，相当于小鼠的寿命延长了20%-40%。研究结论美源康富ABD活性因子能够提升老年小鼠肝脏和心脏中的谷胱甘肽浓度，并延长其寿命。文献来源 Gustavo Bounous, Francine Gervais, Victor Amer, Gerald Batist, Phil Gold，临床研究医学，1989年12期343-349 2. The Antioxidant SystemG. Bounous and J. H. MolsonResearch and Development Department Im

11、munotec Research Ltd., Vaudreuil-Dorion, Quebec, CanadaANTICANCER RESEARCH 23: 1411-1416 (2003)Abstract: The glutathione (GSH) antioxidant system is the principal protective mechanism of the cell and is a crucial factor in the development of the immune response by the immune cells.  Experimenta

12、l data demonstrate that a cysteine-rich whey protein concentrate represents an effective cysteine delivery system for GSH replenishment during the immune response.  Animal experiments showed that the concentrates of whey protein also exhibit anticancer activity. They do this via the GSH pathway

13、, the induction of p53 protein in transformed cells and inhibition of neoangiogenesis.研究主题抗氧化系统研究综述谷胱甘肽抗氧化系统是细胞的首要自我保护机制，也是免疫细胞产生免疫反应的要素。研究数据显示，富含半胱氨酸的美源康富ABD活性因子是有效的半胱氨酸输送系统，为免疫反应补充谷胱甘肽。动物实验 显示ABD活性因子还有抗癌作用，因为谷胱甘肽通路能在癌变细胞中诱导p53蛋白合成，并抑制血管生成。羟基自由基超氧化自由基图1：氧自由基和缓冲系统巨噬细胞（抗原处理细胞）胱氨酸谷氨酰半胱氨酸合成酶1谷氨酰半胱氨酸半胱氨

14、酸谷氨酸谷氨酸盐半胱氨酸半胱氨酸半胱氨酸谷氨酸盐2细胞膜甘氨酸谷胱甘肽合成酶谷胱甘肽谷胱甘肽图2：细胞和血浆氧化还原系统1. 高谷胱甘肽水平时被抑制2. 比谷氨酸过膜速度更快胱氨酸半胱氨酸图3：最重要的含硫化合物：半胱氨酸/胱氨酸氧化还原反应ABD Bioactive CytokinesABD Bioactive Cytokines美国的研究加拿大的研究生成血小板的细胞数量生成血小板的细胞数量大豆蛋白酪蛋白大豆蛋白酪蛋白图4：研究结果表明特殊制备的ABD活性因子（WPC）对于免疫持续性的重要作用肿瘤负荷（肿瘤数/组数）肿瘤负荷（肿瘤数/组数）231结肠谷胱甘肽肉干酪素干酪素专利乳清蛋白提纯食品

15、（宠物饲料）普瑞纳肉干酪素专利乳清蛋白提纯食品专利乳清蛋白提纯食品加拿大的研究美国的研究图5：研究结果显示特殊制备的乳清蛋白提纯饮食对于癌症防护的重要作用。致癌物为二盐酸肼（DMH），该致癌物会导致结肠肿瘤，这些肿瘤与人体中得到的肿瘤非常相似（损害类型和对化学疗法的反应）。在整个二盐酸肼处理的24周前，就开始为小鼠提供不同的饮食，并且一直持续到实验结束。不同食物对体重没有差异化的影响。1. 喂食特殊制备的乳清蛋白提纯饮食的小鼠体内肿瘤质量小于喂食干酪素和宠物饲料的小鼠体内肿瘤质量。2. “这些发现肯定并延展了之前加拿大所做的实验组（Bounous et al, 1991）同时确认了乳制品蛋白，

16、特别是乳清蛋白，能够减少由于二盐酸肼所导致的肠癌。”3. 单个肿瘤质量差异没有显著差异文献来源 G. Bounous and J. H. Molson，抗癌研究，2003年23期1411-1416页3. Oxidative stress and ageing: is ageing a cysteine deficiency syndrome?W. DrögeDivision of Redox Physiology and Aging Research, Deutsches Krebsforschungszentrum, Im Neuenheimer ，GermanyPHIL. TRA

17、NS. R. SOC. B. 360, 2355-2372 (2005)Abstract: Reactive oxygen species (ROS) are constantly produced in biological tissues and play a role in various signaling pathways.  Abnormally high ROS concentrations cause oxidative stress associated with tissue damage and dysregulation of physiological si

18、gnals.  There is growing evidence that oxidative stress increases with age.  It has also been shown that the life span of worms, flies and mice can be significantly increased by mutations, which impede the insulin receptor signaling cascade.  Molecular studies revealed that the insuli

19、n-independent basal activity of the insulin receptor is increased by ROS and downregulated by certain antioxidants.  Complementary clinical studies confirmed that supplementation of the glutathione precursor cysteine decreases insulin responsiveness in the fasted state.  In several clinica

20、l trials, cysteine supplementation improved skeletal muscle functions, decreased the body fat/lean body mass ratio, decreased plasma levels of the inflammatory cytokine tumour necrosis factor a (TNF-a), improved immune functions, and increased plasma albumin levels.  As all these parameters degenerated with age, these findings suggest: