布朗大学同位素地球化学 Carbonisotope(2)

1、Reversed TCA cycleLess fractionation13CCO2 trans fixCeCiFractionation ( ): = 13CCO2- 13Calgae= trans + Ci/Ce *( fix- trans)Transport-limited carbon fixation:Enzyme capacity Transport across membraneCi is very small: Ci Enzyme capacityCi = Ce =fix (29 )Pagani et al., 1999, Science 285, 876Pagani et a

2、l., 1999, Science 285, 876C4 plant expansion in late Miocene (Cerling et al)Tipple and Pagani, 2007CO2 + H2O + CaCO3 Ca2+ + 2HCO3-In most cases, 13C values of soil or aquifer dissolved organic carbon are ca. -25 2-3 . However, in places with C4 vegetation, the values can be up to 10 higher.100010002

3、424131313COCHCOCHCCCFractionation is less than ca. 0.935 for CH4 production by CO2 reduction, but greater for acetate fermentationEnviron. Sci. Technol. 2000, 34, 892-896Jason M. E. Ahad, Barbaba Sherwood Lollar, Elizabeth A. Edwards, Greg F. Slater, and Brent E. SleepEnviron. Sci. Technol. 2000, 34

4、, 892-896Jason M. E. Ahad, Barbaba Sherwood Lollar, Elizabeth A. Edwards, Greg F. Slater, and Brent E. SleepEnviron. Sci. Technol. 2000, 34, 2768-2772Y. Bloom, R. Aravena, D. Hunkeler, E. Edwards, and S. K. FrapeEnviron. Sci. Technol. 2000, 34, 2768-2772Y. Bloom, R. Aravena, D. Hunkeler, E. Edwards,

5、 and S. K. FrapeEnviron. Sci. Technol. 2001, 35, 676-681D. Hunkeler, B. J. Butler, R. Aravena, and J. F. BarkerMonitoring Biodegradation of Methyl tert-Butyl Ether (MTBE) Using Compound-Specific Carbon Isotope AnalysisEnviron. Sci. Technol. 2001, 35, 676-681D. Hunkeler, B. J. Butler, R. Aravena, and

6、 J. F. BarkerMonitoring Biodegradation of Methyl tert-Butyl Ether (MTBE) Using Compound-Specific Carbon Isotope AnalysisRetention TimeConcentrationDay 13Day 2617 (H), 21 (H) - HopaneDay 122PhPrC-23-120-110-100-90DC-2100.40.60.8C oncent r at i on0.2C-2400.40.60.8Co ncentr a t i on0.2C-25-120-110-100-90D0.8C oncent r at i on00.40.60. 2C-26Days510152025 3051015202530DaysC-27510152025 30DaysC-180.200.40.60.8C oncent r at i onC30Days-120-110D00.0050.015-1.2