标准缓冲液pH值与温度对照表

1、标准缓冲液pH值与温度对照表温度 C四草酸氢钾 0.05M邻苯二甲酸氢钾0.05M混合磷酸盐 0.025M硼砂0.01M51.674.006.959.39101.674.006.029.33151.674.006.909.28201.684.006.889.23251.684.006.869.18301.684.016.859.14351.694.026.849.11常用缓冲溶液的配制方法1.甘氨酸-盐酸缓冲液(0.05mol/L )X毫升0.2 mol/L甘氨酸+Y毫升0.2 mol/L HCI ,再加水稀释至200毫升pHXYpHXY2.05044.03.05011.42.45032.43

2、.2508.22.65024.23.4506.42.85016.83.6505.0甘氨酸分子量=75.07 , 0.2 mol/L甘氨酸溶液含15.01克/升2.邻苯二甲酸-盐酸缓冲液(0.05 mol/L )X毫升0.2 mol/L邻苯二甲酸氢钾+ 0.2 mol/L HCl,再加水稀释到20毫升pH(20C)XYpH(20C)XY2.254.0703.251.4702.453.9603.450.9902.653.2953.650.5972.852.6423.850.2633.052.022邻苯二甲酸氢钾分子量 =204.23 , 0.2 mol/L邻苯二甲酸氢溶液含40.85克/升3 .磷

3、酸氢二钠-柠檬酸缓冲液PH0.2mol/L NaHPO (ml)0.1mol/L 柠檬酸(ml)PH0.2mol/L NaHPO (ml)0.1mol/L 柠檬酸 (ml)2.20.4010.605.210.729.282.41.2418.765.411.158.852.62.1817.825.611.608.402.83.1716.835.812.097.913.04.1115.896.012.637.373.24.9415.066.213.226.783.45.7014.306.413.856.153.66.4413.566.614.555.453.87.1012.906.815.454.

4、554.07.7112.297.016.473.534.28.2811.727.217.392.614.48.8211.187.418.171.834.69.3510.657.618.731.274.89.8610.147.819.150.855.010.309.708.019.450.55NaHP研子量=142.98 , 0.2 mol/L 溶液为 28.40 克/升NaHPO 2HO分子量=178.05 , 0.2 mol/L 溶液含 35.61 克/升GHO HO分子量=210.14 , 0.1 mol/L 溶液为 21.01 克/升.4 .柠檬酸-氢氧化钠-盐酸缓冲液PH钠离子浓度mo

5、l/L柠檬酸(g)C6H8O7 H2O氢氧化钠(g)NaOH 97%盐酸mlHCl 浓最终体积L 2.20.2021084160103.10.2021083116103.30.2021083106104.30.202108345105.30.3524514468105.80.45285186105106.50.3826615612610 使用时可以每升中参加1克克酚,假设最后pH值有变化,再用少量50%氢 氧化钠溶液或浓盐酸调节,冰箱保存.柠檬酸C6H8O7 分子量210.14, 0.1 mol/L 溶液为21.01克/升.5.柠檬酸-柠檬酸钠缓冲液(0.1 mol/L )PH0.1mol/L

6、柠檬酸(ml)0.1mol/L柠檬酸钠(ml)PH0.1mol/L柠檬酸(ml)0.1mol/L柠檬酸钠(ml)3.03.218.617.21.42.85.08.25.27.312.73.416.04.013.63.614.95.15.46.45.65.514.53.84.014.013.16.06.95.84.715.36.03.816.24.212.37.76.22.817.24.44.611.410.38.69.718.06.42.06.61.418.64.89.210.8柠檬酸HO:分子量210.14, 0.1 mol/L 溶液为21.01克/升柠檬酸钠Na C6H5O7 - 2HO:

7、分子量294.12, 0.1 mol/L 溶液为29.41克/毫升6.乙酸-乙酸钠缓冲液(0.2 mol/L )pH(18C)0.2mol/LNaAc(ml)0.3mol/LHAc(ml)pH(18C)0.2mol/LNaAc(ml)0.3mol/LHAc(ml)2.60.759.254.85.904.103.81.208.805.07.003.004.01.808.205.27.902.104.22.657.355.48.601.404.43.706.305.69.100.904.64.905.105.89.400.60NaAc 3H2O分子量=136.09 , 0.2 mol/L 溶液为

8、27.22 克/升.7.磷酸盐缓冲液(1)磷酸氢二钠-磷酸二氢钠缓冲液(0.2)PH0.2mol/L NaHPO(ml)0.3mol/LNaHPO (ml)PH0.2mol/LNaHPO(ml)0.3mol/LNaHPO (ml)5.85.96.06.16.26.36.46.56.66.76.86.98.010.012.315.018.522.526.531.537.543.549.555.092.090.087.785.081.577.573.568.562.556.551.045.07.07.17.27.37.47.57.67.77.87.98.061.067.072.077.081.08

9、4.087.089.591.593.094.739.033.028.023.019.016.013.010.58.57.05.3NaHPO 2HO分子量=178.05 , 0.2 mol/L 溶液为 85.61 克/升.NaHPO 2H2O分子量=156.03 , 0.2 mol/L 溶液为 31.21 克/升.PHmol/L/15NaHPO(ml)mol/L/15KHPO (ml)PHmol/L/15NaHPO(ml)mol/L/15KHPO (ml)4.925.295.916.246.476.646.816.980.100.501.002.003.004.005.006.009.909.5

10、09.008.007.006.005.004.007.177.387.738.048.348.678.187.008.009.009.509.759.9010.003.002.001.000.500.250.100NaHPO 2Ho分子量=178.05 , 1/15M溶液为 11.876 克/升.KHPO分子量=136.09 , 1/15M溶液为9.078克/升.8.磷酸二氢钾-氢氧化钠缓冲液(0.05M)X毫升0.2M K2PO4 + Y毫升0.2N NaO刖口水稀释至29毫升pH(20 C)X (ml)Y (ml)pH(20C)X (ml)Y (ml)5.850.3727.052.9636

11、.050.5707.253.5006.250.8607.453.9506.451.2607.654.2806.651.7807.854.5206.852.3658.054.68010. Tris -盐酸缓冲液(0.05M, 50毫升0.1M三羟甲基氨基甲烷( 毫升.9.巴比妥钠-盐酸缓冲液(18C)PH0.04M巴比 妥钠溶液(ml)0.2N盐酸 (ml)PH0.04M巴比妥钠 溶液(ml)0.2N盐酸 (ml)6.87.07.27.47.67.88.08.210010010010010010010010018.417.816.715.313.411.479.397.218.48.68.89.

12、09.29.49.61001001001001001001005.213.822.521.651.130.700.35巴比妥钠盐分子量=206.18;0.04M溶液为8.25克/升25Tris )溶液与X毫升0.1N盐酸混匀后,加水稀释至100PHX(»#)PHX(»#)7.1045.78.1026.27.2044.78.2022.97.3043.48.3019.97.4042.08.4017.27.5040.38.5014.77.6038.58.6012.47.7036.610.38.707.8034.58.808.57.9032.08.907.08.0029.2三羟甲基

13、氨基甲烷Tris HOCH 3NH C 分子量=121.14; 0. 1M 溶液为12.114克/ 升.Tris溶液可从空气中吸收二氧化碳,使用时注意将瓶盖严.11 .硼酸-硼砂缓冲液0.2M硼酸根PH0.05M硼砂 (ml)0.2M硼砂 (ml)PH0.05M硼砂 (ml)0.2M硼酸 (ml)7.41.09.08.23.56.57.61.58.58.44.55.57.82.08.08.76.04.08.03.07.09.08.02.0硼砂NaB. H2O,分子量=381.43;0.05M溶液=0.2M硼酸根含19.07克/升硼酸HBO,分子量=61.84,0.2M溶液为12.37克/升.硼

14、砂易失去结晶水,必须在带塞的瓶 中保存.12 .硼酸-硼砂缓冲液0.2M硼酸根PH0.05M硼砂(ml)0.2M 硼酸(ml)PH0.05M硼砂(ml)0.2M 硼酸(ml)7.41.09.08.23.56.57.61.58.58.44.55.57.82.08.08.76.04.08.03.07.09.08.02.0硼砂NaBQ 10H2O,分子量=381.43;0.05M溶液=0.2M硼酸根含19.07克/升硼酸HBO,分子量=61.84, 0.2M 溶液为12.37克/升.硼砂易失去结晶水,必须在带塞的瓶中保存.13 .甘氨酸-氢氧化钠缓冲液0.05MX毫升0.2M甘氨酸+Y毫升0.2Nn

15、aOH加水稀释至200毫升1 PHXYPHXY8.6504.09.65022.48.8506.09.85027.29.0508.810.05032.09.25012.010.45038.69.45016.810.65045.5甘氨酸分子量=75.07;0.2M溶液含15.01克/升X毫升0.05M硼砂+Y毫升0.2NNaOHfe水稀释至200毫升PHXYPHXY9.3506.09.85034.09.45011.010.05043.09.65023.010.15046.0硼砂 Na2B4O7 10H2O分子量=381.43;0.05M 溶液为 19.07 克/升.14.碳酸钠-碳酸氢钠缓冲液(0.1M)Ca2+ Mg2存在时不得使用|PH0.1MNaCO(ml)0.1MNaHCOil)20 c37 c9.168.77199.409.1228