多功能电转仪

1、文献参考No. 819文献参考No. 8Eppendorf#文献参考No. 8#文献参考No. 8摘要材料和方法PEGMentha piperitaPiperitamultimentha Mentha piperitaPiperita mitchamMentha suaveolens EhrhSHEppendorfMultiporatorSH2AEppendorfSH0.2 mm250 pl EppendorfSH0.2 mm2B#文献参考No. 8#文献参考No. 8#文献参考No. 8方法床冲长度和数量Sato135.0-9.0x105Ec=Vc/(0.75xd)0.7MVc250 pld

2、(cmdDAPI 4 6-Diamidino-2-25 pmPhenylindol4B1066.7 V/cm21.3 V22 1V V533.33 V/cm0.2 mm25 VnI0Aiodine acetamide结果925 ps18 26-72U1- 1.5 V40 sU n V25 ps2x1.5V40 sV/cm=V /cm排列参数的确定1 50ps2 25ps3 20US1.5-3V0.5 V2.5 V1.5V3 Sato3 M. Piperita Piperita mitcham.1.5Vx200床冲电压的确定750 2,500 V/cm8DAPI130 - 60 s40 s10s

3、o -'4Atransmitted4BDAPIx20021文献参考No. 8#文献参考No. 8150 V 170V82% 81%25% - 24%Optimization offusion parametersut 7.5 V40 sU n 170 V25 ps2x7.5 V40 s2U、75V40 sU n V25 ps2x7.5 V40 sVoltage (V) Vitality (%) Fusion rate (%)M.Piperita Piperita mitcham.总结16-27%.6%97%Phytowelt GreenTechnoIodies GmbHw Power,

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5、agfBer1in9Heidelberg: 167-182w GmsserJ W, OllitraultP,OlivaresFusgrO(2000). In Vitro Cell Dev. EQ, Plant.3&434449何 Brewer,E.P.r Saunders, J.A., Angle, J.S., Chanery, R丄“ McIntosh, M.S( 1999). Theoretical and Applied Genetics 99: 761-771W Kao. KN Michayluk MR(1974).Planta 115:355367 Zimmermann U.

6、f Scheurich, P(1981) Plants. 151:26-32遇 Cheng. J. Saunders. J.A.(1995). In Methods in Molecular Biology, Vol. 55: Plant Cell Electroporation and Electrofusion ProtocolsEdited by: J.A.Nickoloff Humana Press Inc., Totowa,NJ Saunders, J.A.9BatesfG.W.(1987) In Cell FusionfSowers. AE.&bJ, Plenum. New

7、 York:497-520Fish. N.KarptA.f Jones.M.G.K (1988) Theor. App;.Genet. 76:260-266tfl FNR-Fachagentur Nachwachsende Rohstoffe e. V. Plenum. New York:497-520.g Smith. M.A.L. McCown, B.(1982/1983). Plant Sci.Lett.28: 149-156z Sa© H.Enomoto, S.,Okaf S.tHosomi,K.tIto, Y.(1993). Plant Cell Reports, 12:546-550m SatotH.fYamada.K.f Hosomif K.,okuyamarS., UzawaMJ shikawa Ho,Y(7996). Plant Science 115:101-107(7)作者 Ellen N