各种缓冲液的配制方法

1、1 .甘氨酸-盐酸缓冲液(0.05mol/L )之答禄夫大 创作时间：二O二一年七月二十九日X毫升0.2 mol/L甘氨酸+Y毫升0.2 mol/L HCI,再加水稀释至 200毫升pHXYPHXY2.05044.03.05011.42.45032.43.2508.22.65024.23.4506.42.85016.83.6505.0甘氨酸分子量=75.07,0.2 mol/L甘氨酸溶液含15.01克/升.2 .邻苯二甲酸-盐酸缓冲液(0.05 mol/L )X毫升0.2 mol/L邻苯二甲酸氢钾 + 0.2 mol/L HCl,再加水稀释到20毫升pH(20C)XYpH(20C)XY2.25

2、4.0703.251.4702.453.9603.450.9902.653.2953.650.5972.852.6423.850.2633.052.022邻苯二甲酸氢钾分子量=204.23,0.2 mol/L邻苯二甲酸氢溶液含40.85克/升3 .磷酸氢二钠-柠檬酸缓冲液pH0.2 mol/L NazHPO(毫升)0.1 mol/L 柠檬酸 (毫升)pH0.2 mol/L Na?HPO(毫升)0.1 mol/L柠檬酸(毫升)2.20.4010.605.210.729.282.41.2418.765.411.158.852.62.1817.825.611.608.402.83.1716.835.

3、812.097.913.04.1115.896.012.637.373.24.9415.066.213.226.783.45.7014.306.413.856.153.66.4413.566.614.555.453.87.1012.906.815.454.554.07.7112.297.016.473.534.28.2811.727.217.392.614.48.8211.187.418.171.834.69.3510.657.618.731.274.89.8610.147.819.150.855.010.309.708.019.450.55NaHPO分子量=14.98,0.2 mol/L 溶

4、液为 28.40 克/升.NaHPQ2H2。分子量=178.05,0.2 mol/L 溶液含 35.01 克/升.CMOTbO分子量=210.14,0.1 mol/L 溶液为 21.01 克/ 升.4 .柠檬酸-氢氧化钠-盐酸缓冲液pH钠离子浓度（mol/L ）柠檬酸（克）CHO DO氢氧化钠（克）NaOH 97%盐酸（毫升）HCl （浓）最终体积（升）2.20.2021084160103.10.2021083116103.30.2021083106104.30.202108345105.30.3524514468105.80.45285186105106.50.3826615612610使用

5、时可以每升中加入 1克克酚，若最后pH值有变动,再用少量50%氢氧化钠溶液或浓盐酸调节，冰箱保管.5 .柠檬酸-柠檬酸钠缓冲液（0.1 mol/L ）pH0.1mol/L柠檬酸（毫升）0.1mol/L柠檬酸钠 （毫升）pH0.1柠檬酸（毫升）mol/L0.1mol/L柠檬酸钠 （毫升）3.018.61.45.08.211.83.217.22.85.27.312.73.416.04.05.46.413.63.614.95.15.65.514.53.814.06.05.84.715.34.013.16.96.03.816.24.212.37.76.22.817.24.411.48.66.42.01

6、8.04.64.810.39.29.710.86.61.418.6柠檬酸 C6H8O7 - H2O:分子量 210.14,0.1 mol/L 溶液为 21.01 克/ 升.柠檬酸钠Na C6H5QNH2O:分子量294.12,0.1 mol/L 溶液为29.41克/毫升.6 .乙酸-乙酸钠缓冲液(0.2 mol/L )pH（18C）0.2mol/LNaAc （毫升）0.3HAc（毫升）mol/LpH（18C）0.2 NaAc （毫升）mol/L0.3 HAc （毫升）mol/L2.60.759.254.85.904.103.81.208.805.07.003.004.01.808.205.27

7、.902.104.22.657.355.48.601.404.43.706.305.69.100.904.64.905.105.89.400.60NaAc 3H2O分子量=136.09,0.2 mol/L 溶液为 27.22 克/升.7 .磷酸盐缓冲液(1)磷酸氢二钠-磷酸二氢钠缓冲液(0.2 )PH0.2mol/LNaHPO（毫升）0.3mol/LNaHPO（毫升）pH0.2mol/LNaHPO（毫升）0.3mol/LNaHPO（毫升）5.88.092.07.061.039.05.910.090.07.167.033.06.012.387.77.272.028.06.115.085.07.3

8、77.023.06.218.581.57.481.019.06.322.577.57.584.016.06.426.573.57.687.013.06.531.568.57.789.510.56.637.562.57.891.58.56.743.556.57.993.07.06.86.949.555.051.045.08.094.75.3mol/L 溶液为85.61克/升.mol/L 溶液为71.64克/升.mol/L 溶液为31.21克/升.NaHPO 2H2O分子量=178.05,0.2NaHPO 2H2O分子量=358.22,0.2NaHPO 2H2O分子量=156.03,0.2(2)磷

9、酸氢二钠-磷酸二氢钾缓冲液(1/15 mol/L )pHmol/L/15Na 2HPO（毫升）mol/L/15KH 2PO（毫升）PHmol/L/15Na 2HPO（毫升）mol/L/15KH 2PO（毫升）4.920.109.907.177.003.005.290.509.507.388.002.005.911.009.007.739.001.006.242.008.008.049.500.506.473.007.008.349.750.256.644.006.008.679.900.106.815.005.008.1810.0006.986.004.00NaHPO。2H2O分子量=178.

10、05,1/15M 溶液为 11.876 克/升.KHPO分子量=136.09,1/15M 溶液为9.078克/升.8 .磷酸二氢钾-氢氧化钠缓冲液（0.05M）X毫升0.2M K2PO + Y毫升0.2N NaOH加水稀释至29毫升pH(20C)X （毫升）Y （毫升）pH(20C)X （毫升）Y （毫升）5.850.3727.052.9636.050.5707.253.5006.250.8607.453.9506.451.2607.654.2806.651.7807.854.5206.852.3658.054.6809 .巴比妥钠-盐酸缓冲液（18C）PH0.04M巴比妥钠溶 ft0.2V盐

11、酸PH0.04M巴比妥钠溶液（毫升）0.2N盐酸（毫升）6.810018.48.41005.217.010017.88.61003.827.210016.78.81002.527.410015.39.01001.657.610013.49.21001.137.810011.479.41000.708.01009.399.61000.358.21007.21100巴比妥钠盐分子量=206.18;0.04M 溶液为8.25克/升10 . Tris -盐酸缓冲液（0.05M,25 C）50毫升0.1M三羟甲基氨基甲烷（Tris ）溶液与X毫升0.1N盐酸混匀后，加水稀释至100毫升.PHX（毫升）P

12、HX（毫升）7.1045.78.1026.27.2044.78.2022.97.3043.48.3019.97.4042.08.4017.27.5040.38.5014.77.6038.58.6012.47.7036.68.7010.37.8034.58.808.57.9032.08.907.08.0029.2三羟甲基氨基甲烷（Tris ） HOCH2 CH2OHHOCH2NH2分子量=121.14;0.1M溶液为12.114克/升.Tris 溶液可从空气中吸收二氧化碳，使用时注意将瓶盖严.11 .硼酸-硼砂缓冲液（0.2M硼酸根）PH0.05M硼砂（毫升）0.2M硼砂（毫升）PH0.05M硼

13、砂（毫升）0.2M硼酸（毫升）7.41.09.08.23.56.57.61.58.58.44.55.57.82.08.08.76.04.08.03.07.09.08.02.0硼砂NaBO H2O,分子量=381.43;0.05M 溶液（=0.2M硼酸根）含19.07 克/ 升.硼酸H2BQ,分子量=61.84,0.2M 溶液为12.37克/升硼砂易失去结晶水，必需在带塞的瓶中保管.11 .硼酸-硼砂缓冲液（0.2M硼酸根）PH0.05M硼砂（毫升）0.2M硼酸（毫升）PH0.05M硼砂（毫升）0.2M硼酸（毫升）7.41.09.08.23.56.57.61.58.58.44.55.57.82.

14、08.08.76.04.08.03.07.09.08.02.0硼砂 NaBO 10H2O,分子量=381.43;0.05M 溶液（=0.2M 硼酸根）含19.07克/升.硼酸HBQ,分子量=61.84, 0.2M 溶液为12.37克/升.硼砂易失去结晶水，必需在带塞的瓶中保管12 .甘氨酸-氢氧化钠缓冲液（0.05M）X毫升0.2M甘氨酸+Y毫升0.2NnaOH加水稀释至200毫升PHXYPHXY8.6504.09.65022.48.8506.09.85027.29.0508.810.05032.09.25012.010.45038.69.45016.810.65045.5甘氨酸分子量=75.07;0.2M 溶液含15.01克/升.13 .硼砂-氢氧化钠缓冲液（0.05M硼酸根）X毫升0.05M硼砂+Y毫升0.2NNaOW口水稀释至200毫升pHXYPHXY9.3H506.09.85034.09.45011.010.05043.09.65023.010.15046.0硼砂NaBQ 10HO,分子量=381.43;0.05M 溶液为19.07克/升.14 .碳酸钠-碳酸氢钠缓冲液（0.1M）Ca2+、Mj+存在时不得使用PH0.1MNsbCQ（毫升）0.1MMHCQ（毫升）20C37 c9.168.77199.409.12289.519.40379.789.50469.