模拟失重大鼠肠系膜小动脉平滑肌细胞电压依赖性钙离子通道电流的解读

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5、 February 25, 2005, 57(1: 27-32 10 2+ KV 上调结合 L-VDC 的下调可使 Ca 内流减少，这可能也是失重下主要阻力性动脉血 管功能与结构下 调的电生理学自体调节机制之一。但 L-VDC 电流密 度的降低是 由于通道表达的降低还是单个通道功能 的改变引起还有待进一步阐明。 参考文献 1 Zhang LF (张立藩， 丫u ZB, Ma J, Mao QW. Peripheral effector mechanism hypothesis on cardiovascular dysfunction after spaceflight. Prog Physi

6、ol Sci 生理科学进展 2001; 32(1:13-17 (Chinese, English abstract. 2 Buckey JC, Lane LD, Levine BD, Watenpaugh DE, Wright SJ, Moore WE, Gaffney FA, Blomqvist CG. Orthostatic in tolera nee after spaceflight. J Appl Physiol 1996; 81(1: 7-18. 3 Looft-Wilson RC,Gisolfi CV. Rat small mese nteric artery fun cti o

7、n after hin dlimb suspe nsion. J Appl Physiol 2000; 88: 11991206. 4 Ma J, Zhang LN, Zhang LF. Vasoco nstrictor resp on sive ness of hi nd body vascular beds is dimini shed in tail-suspe nded rats. J Gravit Physiol 2000; 7:153-154. 5 Delp MD, Collera n PN, Wilkerson MK, McCurdy MR, Muller-Delp J. Str

8、uctural a nd fun cti onal remodeli ng of skeletal muscle microvasculature is in duced by simulated microgravity. Am J Physiol Heart Circ Physiol 2000; 278: 1866-1873. 6 Mao QW, Zha ng LF, Ma J. Observation on arteriolar network structure in soleus of tail-suspended rats. J Gravit Physiol 1997; 4:129

9、-130. 7 8 Zhang LF. Invited review: Vascular adaptation to microgravity: what have we learned? J Appl Physiol 2001; 91: 2415-2430. Somlyo AP, Somlyo AV. Signal transduction and regulation in smooth muscle. Nature 1994; 372: 231-236. 9 Jackson WF. Ion channels and vascular tone. Hypertension 2000; 35

10、:173-178. 10 Fu ZJ 付兆君,Che ng HW, Zha ng LF, Ma J. Cha nges in potassium curre nts of vascular smooth muscle cells isolated from hin dquarter arteries of rats after 4 weeks simulated weightlessness. Acta Physiol Sin 生理学报 2002; 54: 525530 (Chinese, En glish abstract . 11 Morey-Holton ER,Globus RK. Hi

11、 ndlimb un loadi ng rode nt model: technical aspects. J Appl Physiol 2002; 92:1367-1377. 12 He JQ 何加强,Qu JH, Zheng YF, Wang XF. Characteristics of voltage-depe ndent Ca2+ cha nn els in smooth muscle cell membra ne of visceral resista nee vessel in SHRsp.Acta Biophys Sin 生物物理学报 1998; 14(3: 418-427 (C

12、hinese, English abstract. 13 Cox RH Rusch NJ . New expressi on profiles of voltagegated ion cha nn els in arteries exposed to high blood pressure. Microcirculation 2002 ； 9: 243-257. 14 Zha ng LF, Zha ng LN, Me ng QJ, Fu ZJ. Research in differe ntial adaptatio ns of vessels to microgravity. 23rd Ann

13、ual Intern ati onal Gravitational Physiology Meeting; 2002 June, Karolinska Institute, Sweden. ESA SP-501. J Gravit Physiol 2002; 9(1: P55-P58. 15 Yuan JX, Rubin LJ. Altered expression and fun cti on of Kv cha nn els in primary pul monary hyperte nsion. In Archer and Rusch (eds. Potassium Chann els in Cardiovascular Biology. New York: Kluwer Academic/ Ple num Publishers, 2001,821836. 16 Fu ZJ, Xie MJ, Zha