Nematode Extraction s uarkedu：线虫提取方法 uarkeu

1、nematode extraction methods:root extraction techniqueskavitha govindasamykavitha govindasamyjimmy r.richjimmy r.richmaria l .mendesmaria l .mendesa plant-parasitic nematodesampling for nematodessampling and extracting for nematodes serves two purposes: odiagnosis a current problemopredict a future p

2、roblem objectives of sampling and extracting nematodeso identify the nematode o population estimation o diagnose a nematode diseaseo make management decisionso regulatory purposeso research and surveyssome common nematode extraction methodso root samples jar incubation and sieving blending and sievi

3、ngo soil samples baermann funnel modified baermann funnelo foliar samples punching and incubating scissors technique root extraction techniques:jar methodprincipleonematodes are extracted based on their active migration from roots materials requiredo glass jars and capso 325 or finer mesh sieveo lab

4、els and rubber bandso dark chambersample ready for processingwash the rootsdamp dry the rootsweigh the damp-dried rootsplace roots in jars, add waterplace jars in traystore in the dark for 72 hours following incubation, remove from storage and add water collect nematodes on sieverinse nematodes from

5、 sievenematode samples ready for observationadvantages and limitationsadvantageso extracts migratory endoparasitic nematodes o few materials neededo simple procedure to followlimitationso not useful for ectoparasitic nematodeso lack of aeration can be a problemo sieves are needed to concentrate nema

6、todesroot extraction techniques:blending and sievingprincipleo nematodes are released from roots due to maceration.materials requiredo blendero 40 and 325 or 500 mesh sieveso pan or bowlo wire mesho filter papero squeeze bottleo weighing balanceo scissorswash roots wellcut rootsdamp dry the rootswei

7、gh rootsplace roots into blender, cover roots with waterblend at low speed for 10-20 secondspour the roots into 325 or finer mesh sieveset up a baermann trayplace filter paper on the baermann trayadd water to baermann traytransfer the blended roots into the filter paperincubate for 24 hoursremove ro

8、ots from the panpour the water in the dish through a 325 or fine mesh sieveconcentrate nematodes for observation advantages and limitationsadvantages o fast method to extract nematodes from rootso foliar nematodes can also be easily extractedlimitationso chemicals released from the roots can be injurious to nematodeso blending for long time may damage nematodes acknowledgmentso janete brito, department of plant industry, gainesville, floridao frank woods, nematode assay lab, university of florida, gainesville, floridao joey orajay, entomology and nematology departm