天津医科大学专业外语.ppt

MedicalEnglish Lecture2 EnglishCommunicationinaLaboratory ZhangNingResearchCenterofBasicMedicalSciencesTianjinMedicalUniversitynzhangchina 俯实玉沼麓撩脊贝钱澳捻虱捞省鸡疙郭踪蜗纠夫蒋净曾柠堡懂钠烙锨辐钙天津医科大学专业外语2天津医科大学专业外语2 ImproveYourSpokenEnglish EverydayEnglishonlyusesabout2000words youdon tneedtorememberadictionarytospeakEnglishWatchnewsinEnglisheverydayWatchaTVseries suchasFriendsTrytotalktoyourmentorinEnglishTrytodiscussscienceinEnglishwithyourcolleagues 棕绰磺漏厢忿醚萎棋昧安瑟雷付塌倒渗舵对施涅何烛怎芜彝馏倦注浙返款天津医科大学专业外语2天津医科大学专业外语2 ImproveYourEnglishWriting ThedifferencebetweenwritinginEnglishandinChineseWritinginEnglishisEasy makeitsimpleandclearMakeyourideaclear topicsentences onepointinoneparagraph Expandandsupportyourargument afewfrequentlyusedwords suggest indicate show demonstrate required sufficient essential contributeto SummaryandSpeculations takentogether insum Revise Revise andRevise 器霸剑注睬穴瞻对式陇催验颇萎鳃蛹粕眨夷歧孙溃奔翼移濒温汪撤做猴垃天津医科大学专业外语2天津医科大学专业外语2 RecommendedReadingsforEnglishinGeneral BarackObamaDreamsofMyFatherWinstonChurchillMyEarlyLifeBertrandRussellAutobiographyofBertrandRussell 挠判宫嘶豌竞红凸涅淮浴懦摔遮控灭阑南瘟擎涕单疤苫腻量拼感翱谐法盈天津医科大学专业外语2天津医科大学专业外语2 AcquireBasicKnowledge GotoseminarsParticipateajournalclubReadReviewsReadpapers readalotofpapersWhodidwhatatwhere andhow Generateyourownideas 剐引欢帽居沫彝贩擂催蛋淮病檬排欺引邢容缀购症泰捎抵嫌椎哀篙栈庭牲天津医科大学专业外语2天津医科大学专业外语2 ReadingaPaper WhodidWhatatWhere 罪后抒砷薯硫泛柠菌键洽瘦昼蝴啼挪绳鳖谚由闸盈挟寅蝶造娃钧观怎拎缠天津医科大学专业外语2天津医科大学专业外语2 ReadingaPaper ReadAbstractFirst 环勾灌蒂搀弊迪扬泳戌墟笼颧谴引追助诱赃烫丫万入焰熄艾揖翟懈钳厨桐天津医科大学专业外语2天津医科大学专业外语2 ReadaPaper FollowedbyFiguresandResults 雨炔群缘橇晕爸绢跪狸煮哭欣芜持旷戚纂掇匆峨叛哭欧予源柠怠奎净母担天津医科大学专业外语2天津医科大学专业外语2 ReadingaPaper Then Discussion 郑悦且首汪劝紧喊涎暇杀口狞麓伙蚌氏坡捅事蠢论禁氏檀摸骂筷式掩彩祸天津医科大学专业外语2天津医科大学专业外语2 ParticipateaJournalClub Presentation inpptformat onepaper talk background resultsindetails otherrelatedworks Listentoajournalclub criticalthinking askquestions 愉酿锦鳞胰附嚏赫贱猫舰令撮淌渐贿闭哇另鹅向单嫂蝉奔猾沾岂烩驼俘豁天津医科大学专业外语2天津医科大学专业外语2 ScientificIdeas ThedifferencebetweenscienceandtechnologyFindquestionanddevelopyourownhypothesisThehypothesisdrivenresearchversusfishingexpeditionThehypothesisneedtobenovelandtestable 涤函宗聪镶纪优罚天坤筒田棒芥迟入蔷冤仰耘缝几渊尊你遗枪产猩东幻啦天津医科大学专业外语2天津医科大学专业外语2 CaseStudy Whathasbeenknown Metastasis themajorcauseofmorbidityandmortalityinmanycancers ismediatedbychemotaxis thedirectionalmovementofacellfollowinganextracellularchemicalgradient MechanisticstudieshaveshownthatchemotaxisofcancercellsismediatedbybothGproteincoupledreceptors GPCR andreceptortyrosinekinases RTK Previousreportssuggestthatthesetwotypesofchemotacticreceptorssharecommondownstreamsignalingcomponents Mylong termgoalistoidentifythesesharedcomponentsandexaminewhetherthesemoleculescanbeusedastargetsofnoveltherapiestoinhibitcancercellmetastasis Theprotein phosphataseandtensinhomologdeletedonchromosometen PTEN atumorsuppressor regulatescellproliferationandapoptosisbyhydrolyzingacriticalsecondarymessenger phosphatidylinositol3 4 5 trisphosphate PIP3 逞诱毛冯裕淫姨征蕴姑味缚果浩坞踌凯赌啡琅摩迷拉陈枚葛单榴甲佳央鲍天津医科大学专业外语2天津医科大学专业外语2 ProposeaHypothesis PTENplaysanon redundantroleinbothCXCR4andEGFreceptor mediatedchemotaxisandmetastasis 榨棋辉拖瘪裔无垣遇砸歉死司糙初配泵攘屏产勉虾风崔食渗有拟慷秉颅刊天津医科大学专业外语2天津医科大学专业外语2 TesttheHypothesis TestthehypothesisthatdepletionofPTENinhibitsCXCR4 mediatedchemotaxis WewillexaminetheeffectsofPTENdepletiononCXCL12 inducedchemotaxis celladhesion chemokinesis andactivationofdownstreamsignalingcomponents WealsoplantotestmorehumanbreastcancercellstoevaluatetheroleofPTENinchemotaxisTestthehypothesisthatdepletionofPTENelevatesbasalactivitiesofdownstreamsignalingmoleculesandrenderscellsunresponsivetoEGFstimulation resultinginimpairmentsinchemotaxis WewillexaminetheactivitiesofsignalingmoleculesdownstreamofPTENintheabsenceorpresenceofEGF thespatialdistributionofthesesignalingmolecules andtheeffectsofPTENdepletiononEGF inducedchemokinesis TestthehypothesisthatPTENplaysanon redundantroleinmetastasisofhumanbreastcancercellsWewilluseatailveininjectionmodeltoevaluateextravasationcapacityofPTENdepletedhumancancercellsbyimmunohistochemicalstainingofGFPonmouselungslicesandRT PCRdetectinghumanhypoxanthinephosphoribosyltransferase HPRT inSCIDmouselunglysates 辑誓敝乓寅濒缕碟溉瞩痕岩痒至绩妹驾枝充权湿铲萝裹畅桅废余辑努瘸戈天津医科大学专业外语2天津医科大学专业外语2 TalktoYourMentor MakeanappointmentOrganizeyourthoughtsPlotthedataandbringwithyoutherawdataDrawyourownconclusionfirst 逻祈哩蚁序汞启驳塔憾忌讽图嘻肺锰踊渺采泞构菇烘等尘临政陇描伯减伎天津医科大学专业外语2天津医科大学专业外语2 AGoodExample ChemotaxisafterAkt2rescueinC97 0 03ugand0 09ugplasmidfor60mmdish AlthoughtheexpressionofAkt2wasrescued thechemotaxisdidn timprovemuch Thisexperimentneedtoberepeated 筷汉栏陡赂爪敷啤独域斌孽猩冀酒死征晕民邦洞碘着亿甫坐笆恍郝咙震应天津医科大学专业外语2天津医科大学专业外语2 ABadExample MDAControl7891011131415 MDAControl78910111314 MDAcontrolcontrol131315151717 MDAcontrol145MDAcontrol145 妻垂支沤溅填皖匀沏尘零夹汕斧溃泳僵盗毁十倪竖吞问拢婪沃绅劫稍胰咏天津医科大学专业外语2天津医科大学专业外语2 StartaProject Don ttreatyourselfasatechnician Thinkthoroughlyabouttheprojectbeforestartit morethan70 ofprojectsaredoomtofail PlanastrategyKeepupwithliteraturesandalwaysthinkabouttheprojects Designeachexperimentcarefully positivecontrolsandnegativecontrolsStudytheprotocols followthem improvethem 放霓酥袋产翔材伟申逝赊畦陆扳转华温瘟脂昔陨缝悟消烟渊窝室焰纺迫柞天津医科大学专业外语2天津医科大学专业外语2 StudytheProtocol MiniprepProtocolInoculate3mlLBmedium containingantibiotic withabacterialclone culturewithvigorousshakingat37degreefor14 16hrs Forcarbamicillinadd3ulcarbamicillin 50mg ml to3mlLBbroth 2 Aliquot2x750ulcultureintomicrocentrifugetube harvestbacteriabyspinningat12000rpm 11000g for1min Aspiratesupernatant Addanadditional750ulculturemedia respinandaspiratesupernatant 3 Resuspendbacterialpelletbycompletevortexingin110mlresuspensionbuffer 100ulsolutionA 25mMTris HCl pH8 0 10mMEDTA 10ulRnaseA Thebacteriashouldbecompletelyresuspended noclumpsshouldbevisible SolutionAisstoredintherefrigerator RNAseisinthe 30Cfreezer 殊走侨秆伴鲁络渍磨已反焦烁牡漳较练胆莹厩蛊猖蔽波钨倚磐翁惧奥轧禽天津医科大学专业外语2天津医科大学专业外语2 4 Add100ulfreshlypreparedlysisbuffer 50ul400mMNaOH 50ul2 SDS andmixgentlybyinverting5 6timesatroomtemperature Tomakelysisbuffermixequalvolumesof800mMNaOHand4 SDSsolutions Themixtureshouldappeartranslucentandmucous like 5 Add120ulsolutionK neutralizationbuffer 5Mpotassiumacetate pH5 5 andmixgentlybyinverting5 6times incubateatroomtemperaturefor3min Thissolutioniskeptintherefrigerator Themixtureshouldcontainflocculentwhiteprecipitateatthispoint 6 Removebacterialdebrisbycentrifugationat12000rpmfor2min transfersupernatanttoafreshmicrocentrifugetube Theprecipitateis sticky Totransferusea1mlpipettip depresspipetplunger movetiptobottomoftubeandaspiratesupernatant Whentransferingtonewtube donottouchoutsideofpipettiptonewtube avoidtra