课件：LED光源在园艺与药用植物和人工虫草室内培养中的应用.ppt

LED光源在园艺与药用植物和 人工虫草室内培养中的应用,华南师范大学生命科学学学院 李海航,博士、教授、博导 2014年6月21日，广州,广东省植物生长调节剂协会与广州市果树科学研究所 2014年植物生长调节剂与现代农业研讨会,植物的种子萌发、幼苗生长、形态建成、开花结果等过程都需要光,不同的生物及不同生长发育期需要不同的光质与光强,降温,光在植物与食用菌等的室内培养中广泛应用,光照,研究内容 非洲菊组织培养 不定芽诱导、芽增殖和生根 铁皮石斛和金钗石斛组培繁殖 原球茎增殖、生长、分化 虫草子实体形成,生长,功能成分 子实体的诱导形成 生长：高、鲜重、干重 生物活性物质: 虫草素,多糖,类胡萝卜素,I - Gerbera hybrida 组织培养,Xidian Liu 刘西典,Jianzong Peng 彭建宗,Xiaojing Wang 王小菁,Results shoot multiplication,Explant:,Dedifferentiation,callus,Redifferentiation,Single shoot,Cluster shoots,Rooting,Plantlets,Multiplication Division,Adventitious buds,LED treatment,Shoot multiplication procedure,Compared to WFL, no significant differences were observed in the treatments of V1,V2, V5 and V6. V3 and V4 decreased the FW of the shoots. V6 increased dry weight significantly.,Fresh weight and dry weight,Height and number of shoots,All LEDs could increase the height of shoots except V5. But No.of shoots/explant had no significant different.,Chlorophyll content,All LEDs induced chlorophyll biosynthesis significantly, compared with WFL,W,V1,V2,V3,V4,V5,V6,WFL,35 days cultured in vitro under LED,V1 V4,V3 V6,V2 V5 WFL,35 days cultured in vitro under LED,Explant:,Dedifferentiation,callus,Redifferentiation,Rooting,Plantlets,Results Root induction,Receptacle Multiplication Division,Adventitious buds,Cluster shoots Root induction under LED,Single shoot,V3 treatment decreased FW and DW.,V2 treatment increased FW and DW significantly.,Fresh weight and Dry weight,Heights of shoots under V1, V2, V3 and V4 were significantly higher than control.,V2 had the most number of roots compared with others.,Shoot height and root number,V3, V4 and V5 promoted the root activity significantly. V2 increased the maximum root length.,Root activity and maximum root length,V1,V2,V3,V4,V5,V6,WFL,Seedlings 35 d after rooting induction,Survival rate,Height (cm),No. of leaves,V1 V2 V3 V4 V5 V6 WFL,100% 100% 100% 100% 100% 100% 100%,9.460.22 a 9.770.22 a 8.190.34 b 9.540.30 a 8.020.27 b 8.250.48 b 7.090.23 c,5.190.23 b 4.940.11 b 6.060.19 a 6.000.35 a 5.310.24 b 6.190.20 a 4.600.27 b,Results Transplantation,15 days after transplantation, no difference on seedlings survival rates. All LED treated seedlings were significant higher and number of leaves increased.,V4,V1 V5,V2 V6,V3 WFL,Transplanted seedlings grown for 15 days, Among 6 LEDs, V1,V2 and V6 were suitable for shoot,multiplication and V2 was good for root induction., Survival rates of seedlings from all LED treatments,had no difference after seedling transplantation. But the seedlings grown under LEDs were higher with more leaves than WFL.,Conclusions,Jie Ye 叶婕,Hongmei Bao 包红梅,QingshengYe 叶庆生,II - Dendrobium officinale组培繁殖,Tissue culture process,shoots,Plantlets(1-2cm),seedling (7-8cm),germination 1 month,growth and differentiation 2 month,seeds growth 1 month,protocorms shoot elongation 3 month,Plantlets,27,LED treatment,FW of plantlet(g),bc abc c c,After 90 days growth, FW of plantlet under V6 was the highest, but there were no significant differences compared to control.,30 days,60 days,90 days,0.1 0.0,0.2,0.3,V1 V3,V2 V4,V6,V5 FL,a,d d,a,aba,a,b,abab a,ab,a c cbcab,FW of plantlets,V6,DW of plantlet(g),DW of plantlets under V6 was significantly higher than others after 90 days treatment.,30 days,60 days,90 days,0.00,0.01,0.02,0.03,ab ab,ab,b,a,aa,a,a a,a,V1,V2,V3,V4,V6,V5 FL,ab,a,c,abcaba a ab,DW of plantlets c,V6,Height of plantlet(cm),There were some different in plantlet height after 30 and 60 days irradiation under LED, the different disappeared after 90 days.,30days,60days,90days,0.5 0.0,1.5,2.0,V1,V2,V4 V6,V3 V5 FL,a,a,a,a,bab,a,a,c bc,a a,a,a a,bcbc a,bc bc,Height of plantlets a,90天后差异不显著,Stem diameter,(cm),bcbc cd,Similar results were observed in stem diameter,30days,60days,90days,0.0,0.2,0.3,V2 V4 V6,V1 V3 V5 FL,d,cd,a,ab,ab,b,d,ab a,a a,a ab,ab abab,a,b,Stem diameter,V6,Tillering,Compared with other treatments, number of tillering under V6 was significantly higher than FL.,60days,90days,0,1,2,a,a,V1,V2,V3,V4,V6,V5 FL,a a,a,a,b,bc,a,bc bc,d,c,a,Tillering,分蘖,V6,V6,Roots of plantlet,After 60 days cultivation, there were more roots under V6.,60days,90days,2 0,4,V1 V3,V2 V4,V5,V6,FL,a a,a,a,a,cd,a,ab b,d,ab,c,a,Roots number per plantlets a,Growth of plants after 30,days under LED.,Growth of plants after 60 days,cultivation under LED.,Growth of plants after 90 days,cultivation under LED., Among 6 LED, V6 were suitable for plantlet growth,it promoted the DW, diameter, tillering and rooting in Dendrobium officinale.,Conclusions,III - Cordyceps militaris,ZhiliYi 易智莉,Haihang Li 李海航,本实验室培养的蛹虫草,原基诱导形成,材料与方法,材料与方法,子实体生长实验,材料与方法,甲醇浸提,LED光照处理,结果与讨论,1. 黑暗、红光、远红光下不能形成子实体原基,2. 蓝光和含蓝光的LED组合都能诱导原基形成。表明蓝光在子实体原基诱导形成中起决定性作用,3. LED白光（R/B=2/1）和 LED处理（R/B=2/1）对原基的诱导形成较好,1.光质对子实体原基形成的影响,处理鲜重最高,其他LED处理组与荧光对照组无显著差异,远红光下鲜重最低,结果与讨论,2.光质对子实体生长（鲜重）的影响,（R/B/Fr=4/2/4）干重较高,远红光下干重最低,其次是红光, -处理都含蓝光，干重无显著差异，但都高于远红光和红光,结果与讨论,3.光质对子实体生长（干重）的影响,结果与讨论, 各处理的子实体平均高度无统计学差异,处理的高度略高于其它,4.光质对子实体生长（平均高度）的影响,I. FR (100%) II. R (100%),VII. R:B:FR 1:6:3 VIII. B (100%),III. R:B:FR 4:2:4 IV. R:B:FR 3:2:5,V. R:B:FR 3:3:4 VI. R:B:FR 1:4:5,IX. LED white X. Fluorescent,LED白光虫草素含量最高，比对照高62%； 其次是LED红光，比对照高60%,其他LED组合比荧光对照都有不同程度的提高。, 红光有利于提高虫草素的含量，蓝光对虫草素积累的作用不显著。,结果与讨论,5.光质对虫草素含量的影响, 各光照处理下虫草多糖的含量无统计学差异。,结果与讨论,6.光质对虫草多糖含量的影响, LED蓝光下类胡萝卜素含量最高，比对照组提高了51.6%。,LED远红光下类胡萝卜素含量最低，比荧光对照低54.3%。 其次是红光，比对照低46.4%。,蓝光和含蓝光的LED处理，类胡萝卜素都比对照高，且蓝光比例越高，类胡萝卜素含量越高。,结果与讨论,7.光质对类胡萝卜素含量的影响,光诱导色素合成,光诱导子实体原基形成 （形态建成）,色素的形成发生在光照后4-4.5 h内，至20 h色素含量持续增加,结果与讨论,光诱导虫草色素形成的动态研究,光照1天内色素合成启动,色素合成的启动发生在光照后4-4.5小时内,结果与讨论,光诱导虫草色素形成的动态研究,蛹虫草色素HPLC-ESI-MS检测的总离子流图,色素成分与光诱导关键步骤待确定,结论,1. 黑暗、红光、远红光下不能形成子实体原基；蓝光在子实体原基的诱导形成中起着决定性作用，蓝光和含蓝光的LED组合光都能诱导原基形成。,2. LED