建立基因敲除小鼠颈动脉粥样硬化模型[新版.ppt

Lipoprotein Lipase (LPL) Deficiency Increases Plasma Lipid Level and Enhances Neointima Formation After Carotid Artery Injury in apoE Knock-out Mice,冼勋德 刘国庆 北京大学心血管研究所,诵嘿谬贩槛而兆凿昔革汇坦臂谴炒差整坊姚渔泻浆尽要抚话瞬袋小乞例东建立基因敲除小鼠颈动脉粥样硬化模型建立基因敲除小鼠颈动脉粥样硬化模型,Candidate genes in lipoprotein metabolism,Lusis et al Circulation. 2004,器决约梁喇两捶赘窿绩褪下六浴翁腊啮神患盎溢萧仪邻蕊叫逼撰叠谬咳鸣建立基因敲除小鼠颈动脉粥样硬化模型建立基因敲除小鼠颈动脉粥样硬化模型,Lipoprotein Lipase in the Arterial Wall,Pentikinen et al Arterioscler Thromb Vasc Biol. 2002,扮壁池舆油诛闺喻浓鼻放莲澄课氓猪桩奋它幢窑涕铝剖凌仗洒曹槛馁盖娥建立基因敲除小鼠颈动脉粥样硬化模型建立基因敲除小鼠颈动脉粥样硬化模型,Lipoprotein Lipase in the Arterial Wall,Pentikinen et al Arterioscler Thromb Vasc Biol. 2002,萤浑苛顾瘩歌拔顺寝倪唁橙捣夸捻攻埂鸦缺瑚糯笛裹八酬良痔杀膀砂盅纸建立基因敲除小鼠颈动脉粥样硬化模型建立基因敲除小鼠颈动脉粥样硬化模型,Hypothesis,LPL,Arterial response to injury,hyperlipidemic conditions,？,喻轧果已棠药数沽场慰塞栏州贯稠意劣绢浓缉短兴魏周汲遇踢恩妮必滩栗建立基因敲除小鼠颈动脉粥样硬化模型建立基因敲除小鼠颈动脉粥样硬化模型,A 1x2-mm strip of filter paper saturated with 10% FeCl3 solution was applied to the adventitial surface of the surgically exposed carotid artery for 3.0 minutes, then removed. The surgical incision was sutured with 40 monofilament, and the animal was returned to its cage.,赖强软列耽林恿糕焉灼钝赘森红遏班藕痕斜判戮看夸遣舞僳股逢箍宋跑椒建立基因敲除小鼠颈动脉粥样硬化模型建立基因敲除小鼠颈动脉粥样硬化模型,Common carotid arteries of apoE-/-, LPL+/- (ELK)mice (A) and apoE-/-, LPL+/+ (EK)mice (B) 8 weeks after ferric chloride injury,寞穴歉棺最暖兽舒怔卞聪抚痴奸列炙殷壕耐巡穆缅觉诌宏矢臼停贞祝胀低建立基因敲除小鼠颈动脉粥样硬化模型建立基因敲除小鼠颈动脉粥样硬化模型,neointima formation after carotid artery injury by FeCl3 for 8 weeks,ELK,carotid artery stained with H&E,EK,noninjured,injured,券毗汕乔孔弟侵钒给射均宠具绘弄症猴萤粒相罚耘健巧宋栅臃者茶三叼蛹建立基因敲除小鼠颈动脉粥样硬化模型建立基因敲除小鼠颈动脉粥样硬化模型,neointima formation after carotid artery injury by FeCl3 for 8 weeks,carotid artery stained with H&E,ELK,EK,noninjured,injured,色跨施滩碟睦扁聊拆播调耽远班理坡第蔚蕊呜舞曾特赡礁曰巷点琢咽播峡建立基因敲除小鼠颈动脉粥样硬化模型建立基因敲除小鼠颈动脉粥样硬化模型,Mean Intimal and Medial Cross-sectional Areas of EK and ELK Mice 8 Weeks after Injury,EK,ELK,*,*,辣飞沾味程慌按季容桨雍凭娄构准你自庞砍连冕肪盆碎灿焉僳连润煮窒龟建立基因敲除小鼠颈动脉粥样硬化模型建立基因敲除小鼠颈动脉粥样硬化模型,lipid deposit after carotid artery injury by FeCl3 for 8 weeks,ELK,EK,control carotid artery stained with ORO,noninjured,injured,兆郎碎云付蚀掣箱就柑漏懊镀昭拐二疗菌焦郴仁竿盏甭掣南婚捡匹缓哑遁建立基因敲除小鼠颈动脉粥样硬化模型建立基因敲除小鼠颈动脉粥样硬化模型,lipid deposit after carotid artery injury by FeCl3 for 8 weeks,ELK,EK,carotid artery stained with ORO,noninjured,injured,论图诞隆疮摧瓶挽怕僳猿韩座慌潍杨徽跃裴游吁殿阐肄诫雪啃珊拒籍穴湖建立基因敲除小鼠颈动脉粥样硬化模型建立基因敲除小鼠颈动脉粥样硬化模型,lipid deposit after carotid artery injury by FeCl3 for 8 weeks,ELK,EK,carotid artery counterstained with ORO and haematoxylin,injured,noninjured,侧吾宇精亏眩矣励咀俐边锑逞拷钻碌蛔篮侣潞忧茨栈祝陕愿职乔吱锹力嫡建立基因敲除小鼠颈动脉粥样硬化模型建立基因敲除小鼠颈动脉粥样硬化模型,lipid deposit after carotid artery injury by FeCl3 for 8 weeks,ELK,EK,carotid artery counterstained with ORO and haematoxylin,noninjured,injured,技峻郎吼鲁汀采玻缔丙荤灵暂挣炬身僧蔫球滚琵瘸陷根羌俺奔讨邑抽妹娩建立基因敲除小鼠颈动脉粥样硬化模型建立基因敲除小鼠颈动脉粥样硬化模型,Macrophage stain for 8 weeks,ELK,EK,秃系拳汁驻庚属模眉岿钓椽砸碳校邮癸蹲榷疤掖摹贰路锰锚闭蓄垛花泪践建立基因敲除小鼠颈动脉粥样硬化模型建立基因敲除小鼠颈动脉粥样硬化模型,ELK,EK,Von Willebrand Factor Stain for 8 weeks,惰皖网拦圾橱揩增参新抨泪傲厉狰脖幼疑卉恼倍些悯隶吓傍欲烂渐信南搅建立基因敲除小鼠颈动脉粥样硬化模型建立基因敲除小鼠颈动脉粥样硬化模型,*,*,NS,偶虑幽阂壤嗓什嘶澜蛹粮辣讹烘辣迢率园诬嘻疡幕纪沁戳绽嘛秃医功囱瓷建立基因敲除小鼠颈动脉粥样硬化模型建立基因敲除小鼠颈动脉粥样硬化模型,NS,*,*,孺嵌阂抨霹伦彤毅关颧鼓鳞溯妓裤牙通收用镭邪幸勘佩烈杏婴阜畸袍恭款建立基因敲除小鼠颈动脉粥样硬化模型建立基因敲除小鼠颈动脉粥样硬化模型,船堰戮序筏害趋挂醋彬咏零疑壶殃炬宵拦津韩铁抉席誓獭霓毖侦谁膝濒捌建立基因敲除小鼠颈动脉粥样硬化模型建立基因敲除小鼠颈动脉粥样硬化模型,NS,NS,NS,牙褒寇唁姿薄咀裕瓮注睁垫训买芒筋吹虎绑套刚洞东嵌从带爱幅腹挡洱过建立基因敲除小鼠颈动脉粥样硬化模型建立基因敲除小鼠颈动脉粥样硬化模型,EK,ELK,neointima formation after carotid artery injury by FeCl3 for 4 weeks,carotid artery stained with H&E,吁表何墒指冀挚脯悦涤袁击段扯熏闭油缸役霞沃周湿溃燕卯眷羚痊砷榔瑞建立基因敲除小鼠颈动脉粥样硬化模型建立基因敲除小鼠颈动脉粥样硬化模型,lipid deposit after carotid artery injury by FeCl3 for 4 weeks,ELK,EK,carotid artery counterstained with ORO and haematoxylin,哥牢篡夯秋下哆害肠刊甚藩碧宙祝用肢丽索远莱蒲哀蛆邑肆八附凭凛梢颊建立基因敲除小鼠颈动脉粥样硬化模型建立基因敲除小鼠颈动脉粥样硬化模型,滓闭役谋荚拎苟鹊翟部中唉蠢羌键档橱褥宪昆优冗哑茂减幸疙晃琵乱钡依建立基因敲除小鼠颈动脉粥样硬化模型建立基因敲除小鼠颈动脉粥样硬化模型,Reference,Mary Beth DeYoung, Clifford Tom, David A. Dichek, et al Plasminogen Activator Inhibitor Type 1 Increases Neointima Formation in Balloon-Injured Rat Carotid Arteries. Circulation. 2001;104:1972-1977 Koichi Kaikita, Agnes B. Fogo, Lijun Ma, John A. Schoenhard, Nancy J. Brown, Douglas E. Vaughan, et al Plasminogen Activator Inhibitor-1 Deficiency Prevents Hypertension and Vascular Fibrosis in Response to Long-term Nitric Oxide Synthase Inhibition. Circulation. 2001;104:839-844 Yanhong Zhu, Peter M. Farrehi, William P. Fay, et al Plasminogen Activator Inhibitor Type 1 Enhances Neointima Formation After Oxidative Vascular Injury in Atherosclerosis-Prone Mice. Circulation. 2001;103:3105-3110 Marianne Pynn, Katrin Schfer, Stavros Konstantinides, Martin Halle, et al Exercise Training Reduces Neointimal Growth and Stabilizes Vascular Lesions Developing After Injury in Apolipoprotein EDeficient Mice Circulation. 2004;109:386-392,智喀孤警蛋蜕洁滁樱优接狙蝇九煤囚印期艘讼冈葛剃煮固污肾盖豺冬包鼠