简介WatersUPLC故障排除

1、,Waters UPLC Troubleshooting Training,Agenda,ACQUITY UPLC Family ：UPLC & H-Class Waters UPLC System UPLC Solvent Management (BSM) UPLC Sample Management (SM) Waters ACQUITY H-Class System Quaternary Solvent Manager (QSM) Sample Manager-Flow Through Needle (SM-FTN) Waters UPLC Detector Troubleshootin

2、g Training Waters UPLC PDA and PDAe TUV FLR ELSD Open Discussion,ACQUITY UPLC Family,ACQUITY UPLC System,ACQUITY UPLC System,Detectors: Optical and/or Mass Spec Tunable UV or Photodiode Array Optimized flow cell for UPLC High speed data sampling,Column Manager: Innovative pivot design Positions colu

3、mn to detector E-Cord connection,Sample Manager: Low dispersion XYZZ Format Fast cycle times Low carryover Plates and/or vials Optional Sample Organizer,Binary Solvent Manager: High pressure blending Binary gradients Four solvent choices On-line degassing Low dispersion design UPLC pressure capabili

4、ties,Sample Organizer: (option) Expands capacity (22/15/8) Shuttles plate feed Heated/chilled,System Considerations: Small Footprint Redesigned tubing and fittings Consolidated waste management Integrated system diagnostics Connections Insight remote diagnostics,Fluidics and Connections,Tubing manag

5、ement Chromatographic solvents: A1/A2, B1/B2 Two Sample Manager wash solvents Seal Wash Common fittings and tools for users Column inlets/outlets and fittings Finger tight, turn (use 1/4” wrench) Dedicated tubing 3 piece compression screw and ferrule Small footprint 13” wide 24.25” with Sample Organ

6、izer,UPLCSolvent Manager,ACQUITY UPLC System Solvent Delivery,15 K psi performance Accurate and precise flow rates Accurate and precise composition Low delay volume for gradient applications 120 L System Volume Long term reliability,Binary Solvent Manager Components,Filter/ Mixer/ Tee Assembly,“A” P

7、ump Accumulator,“A” Pump Solvent Select Valve,“B” Pump Solvent Select Valve,“A” Pump Primary,“B” Pump Primary,“B” Pump Accumulator,Six Channel Degasser,Intelligent Intake Check Valves,ACQUITY UPLC System From Serial to Parallel Flow,Two independently driven primary and accumulator piston drives Sepa

8、rate drive motors Two transducers Two inlet check valves Four Solvents Capability A1 or A2 and B1 or B2 Two solvents per gradient Six Channel Degasser 4 chromatographic eluents 2 solvents for Sample Manager Strong & weak wash,Binary Solvent Manager Interactive Display,Recommended Solvents List,ACQUI

9、TY UPLC System recommended Solvents MethanolMethanol/water mixtures WaterAcetonitrile/water mixtures Acetonitrile (ACN)Isopropanol (IPA) Sample diluents (in addition to the solvents listed above) Dimethyl sulfoxide (DMSO) Dimethylformamide (DMF) Additives / Modifiers 0.2% formic acid0.1% trifluoroac

10、etic acid (TFA) 0.1% triethyl amine (TEA)0.1% hexafluorobuteric acid 10mM phosphate buffer10mM ammonium bicarbonate 50mM ammonium hydroxide50mM ammonium acetate 0.1% Ethylenediaminetetraacetic acid (EDTA) Cleaners Phosphoric acid (=30%)Sodium hydroxide (=1M) ACQUITY UPLC System non-recommended Solve

11、nts Methylene-ChlorideChloroform Strong acids 5% Ethyl AcetateToluene Chlorinated solvents: (Trichlorobenzene),UPLCSample Manager,Sample Manager Fluidics Tray,Pressure Transducer Valve Manifold,Drive Gears,Wash Syringes Weak Wash Strong Wash,Metering Syringe Drive Assembly,Metering Syringe Valve 3 W

12、ay Rotary Valve,Metering Syringe Assembly,Sample Syringe 50 uL volume PN 410001348 100 L volume PN 700002570 250 L volume PN 410001347 for use with 50 L loop,Column Heating Compartment,ACQUITY In-Line Filter,19,Column In-Line Filter Assembly,Column In-Line Filter Assembly,Column Heating,Sample Manag

13、er Flow Path,Available Loops,DescriptionP/NStatus 1 L Loop430001516Optional 2 L Loop430001264Optional 5 L Loop430001233Optional 10 L Loop430001326Standard 20 L Loop430001320Optional 50 L Loop430001325Optional 100 L Loop170001792(Kit) Optional 250 L Loop176001780(Kit) Optional,Volume Configuration Me

14、nu,Before running the Characterize Needle and Loop Volume function, make sure the proper volumes are configured in the Volumes Configuration menu. The above values are standard for currently shipping ACQUITY system.,Recommended Injection Volumes,Loop Sizes and Injection Modes,Recommended PLNO inject

15、ion volume 20% to 75% of loop volume Recommended Partial Loop Injection 20% to 50% of loop volume,ACQUITY H-Class System,QSM Quaternary Solvent Manager,SM-FTN Sample Manager-Flow Through Needle,CH-A High Temperature Column Heater Active Pre-Heating,Detectors TUV, PDA ePDA, FLR, ELSD,The ACQUITY H-Cl

16、ass System consists of the following: Quaternary Solvent Manager (QSM) Four solvent low pressure gradient mixing Supports UPLC Pressures Low gradient delay volume SM-FTN (Sample Manager-Flow Through Needle) One injection mode- direct inject type Much simpler- Just specify injection volume Excellent

17、recovery and precision CH-A High temperature column heater with active pre-heating,ACQUITY H-Class System,QSM Fluidics-Front View,Primary Head,Accumulator Head,Vent Valve,100ul Mixer,Gradient Proportioning Valve Pairs,Low Pressure Manifold,Degasser Chambers,Seal Wash Pump,QSM Console Top Level Menu,

18、ACQUITY H-Class System,SMDI Front Panel Fluidics,Metering Syringe Valve,Check Valve and Union,Inject Valve,Wash Solvent Inlet Line (not degassed),Leak Sensor Connections,Sample Metering Syringe,SDMI Inject Valve Connections,To Check Valve,To CH-A and Column,Stationary Side Tube,From Pump,To Pressure Transducer,To Sample Needle,Sample Compartment,Power and Status LEDs,Plate Selector Switch,Rotary Tray (Plate Holder),Magnetic Door Sensor,Power Switch,SM-FTN Interactive Display Menu,CH-A,Improved Door Gasket,Added Tubing Gasket (top and bottom) For 3rd party Mass Spec