标准缓冲液pH值与温度对照表

标准缓冲液pH值与温度对照表温度℃四草酸氢钾0.05M邻苯二甲酸氢钾0.05M混合磷酸盐0.025M硼砂0.01M51.674.006.959.39101.674.006.029.33151.674.006.909.28201.684.006.889.23251.684.006.869.18301.684.016.859.14351.694.026.849.11常用缓冲溶液的配制方法1．甘氨酸–盐酸缓冲液〔0.05mol/L〕X毫升0.2mol/L甘氨酸+Y毫升0.2mol/LHCI，再加水稀释至200毫升pHXYpHXY2.02.42.62.85050505044.032.424.216.83.03.23.43.65050505011.48.26.45.0甘氨酸分子量=75.07，0.2mol/L甘氨酸溶液含15.01克/升。2．邻苯二甲酸–盐酸缓冲液〔0.05mol/L〕X毫升0.2mol/L邻苯二甲酸氢钾+0.2mol/LHCl，再加水稀释到20毫升pH(20℃XYpH(20℃XY2.22.42.62.83.0555554.0703.9603.2952.6422.0223.23.43.63.855551.4700.9900.5970.263邻苯二甲酸氢钾分子量=204.23，0.2mol/L邻苯二甲酸氢溶液含40.85克/升3．磷酸氢二钠–柠檬酸缓冲液pH0.2mol/LNa2HPO4〔毫升〕0.1mol/L柠檬酸〔毫升〕pH0.2mol/LNa2HPO4〔毫升〕0.1mol/L柠檬酸〔毫升〕2.22.42.62.83.03.23.43.63.84.04.2

4.44.64.85.00.401.242.183.174.114.945.706.447.107.718.288.829.359.8610.3010.6018.7617.8216.8315.8915.0614.3013.5612.9012.2911.7211.1810.6510.149.705.25.45.65.86.06.26.46.66.8

7.07.27.47.67.88.010.7211.1511.6012.0912.6313.2213.8514.5515.4516.4717.3918.1718.7319.1519.459.288.858.407.917.376.786.155.454.553.532.611.831.270.850.55Na2HPO4分子量=14.98，0.2mol/L溶液为28.40克/Na2HPO4-2H2O分子量=178.05，0.2mol/L溶液含35.01克/C4H2O7·H2O分子量=210.14，0.1mol/L溶液为21.01克/4．柠檬酸–氢氧化钠-盐酸缓冲液pH钠离子浓度〔mol/L〕柠檬酸〔克〕C6H8O7·H2O氢氧化钠(克)NaOH97%盐酸〔毫升〕HCl〔浓〕最终体积〔升〕①2.23.13.34.35.35.86.50.200.200.200.200.350.450.38210210210210245285266848383831441861561601161064568105126101010101010

10①使用时可以每升中参加1克克酚，假设最后pH值有变化，再用少量50%

氢氧化钠溶液或浓盐酸调节，冰箱保存。5．柠檬酸–柠檬酸钠缓冲液〔0.1mol/L〕pH0.1

mol/L柠檬酸〔毫升〕0.1

mol/L柠檬酸钠〔毫升〕pH0.1

mol/L柠檬酸〔毫升〕0.1

mol/L柠檬酸钠〔毫升〕3.03.23.43.63.84.04.24.44.64.818.617.216.014.914.013.112.311.410.39.21.4

2.84.05.16.06.97.78.69.710.85.05.25.45.65.86.06.26.46.68.27.36.45.54.73.82.82.01.411.812.713.614.515.316.217.218.018.6柠檬酸C6H8O7·H2O：分子量210.14，0.1mol/L溶液为21.01克/升。柠檬酸钠Na3C6H5O7·2H2O：分子量294.12，0.1mol/L溶液为29.41克/毫升。6．乙酸–乙酸钠缓冲液〔0.2mol/L〕pH(18℃0.2

mol/L

NaAc(毫升)0.3

mol/LHAc(毫升)pH(18℃0.2

mol/L

NaAc(毫升)0.3

mol/L

HAc(毫升)2.63.84.04.24.44.60.751.201.802.653.704.909.258.808.207.356.305.104.85.05.25.45.65.85.907.007.908.609.109.404.103.002.101.400.900.60Na2Ac·3H2O分子量=136.09，0.2mol/L溶液为27.22克7．磷酸盐缓冲液〔1〕磷酸氢二钠–磷酸二氢钠缓冲液〔0.2〕pH0.2

mol/LNa2HPO4(毫升)0.3

mol/LNaH2PO4(毫升)pH0.2

mol/LNa2HPO4(毫升)0.3

mol/LNaH2PO4(毫升)5.85.96.06.16.26.36.46.56.66.76.86.98.010.012.315.018.522.526.531.537.543.549.555.092.090.087.785.081.577.573.568.562.556.551.045.07.07.17.27.37.47.57.67.77.87.98.061.067.072.077.081.084.087.089.591.593.0

94.739.033.028.023.019.016.013.010.58.57.05.3Na2HPO4·2H2O分子量=178.05，0.2mol/L溶液为85.61克/Na2HPO4·2H2O分子量=358.22，0.2mol/L溶液为71.64克/Na2HPO4·2H2O分子量=156.03，0.2mol/L溶液为31.21克/〔2〕磷酸氢二钠–磷酸二氢钾缓冲液〔1/15mol/L〕pHmol/L/15Na2HPO4(毫升)mol/L//15KH2PO4(毫升)pHmol/L//15Na2HPO4(毫升)mol/L//15KH2PO4(毫升)4.925.295.916.246.476.646.816.980.100.501.002.003.004.005.006.009.909.509.008.007.006.005.004.007.177.387.738.048.348.678.187.008.009.009.509.759.9010.003.002.001.000.500.250.100Na2HPO4·2H2O分子量=178.05，1/15M溶液为11.876克/KH2PO4分子量=136.09，1/15M溶液为9.078克/8．磷酸二氢钾–氢氧化钠缓冲液〔0.05M〕X毫升0.2MK2PO4+Y毫升0.2NNaOH加水稀释至29毫升pH(20℃X(毫升)Y〔毫升〕pH(20℃X(毫升)Y〔毫升〕5.86.06.26.46.66.85555550.3720.5700.8601.2601.7802.3657.07.27.47.67.88.05555552.9633.5003.9504.2804.5204.6809．巴比妥钠-盐酸缓冲液〔18℃pH0.04M巴比妥钠溶液0.2V盐酸pH0.04M巴比妥钠溶液(毫升)0.2N盐酸(毫升)6.87.07.27.47.67.88.08.2100

10010010010010010010018.417.816.7

15.313.411.479.397.218.48.68.89.09.29.49.61001001001001001001001005.213.822.521.651.130.700.35巴比妥钠盐分子量=206.18;0.04M溶液为8.25克/升10．Tris–盐酸缓冲液〔0.05M，25℃50毫升0.1M三羟甲基氨基甲烷〔Tris〕溶液与X毫升0.1N盐酸混匀后，加水稀释至100毫升。pHX(毫升)pHX(毫升)7.107.207.307.407.507.607.707.807.908.0045.744.743.442.040.338.536.634.532.0

29.28.108.208.308.408.508.608.708.808.9026.222.9

19.917.214.712.410.38.57.0三羟甲基氨基甲烷〔Tris〕HOCH2CH2OHHOCH2NH2

C分子量=121.14;0.1M溶液为12.114克/升。Tris11．硼酸–硼砂缓冲液〔0.2M硼酸根〕pH0.05M硼砂〔毫升〕0.2M硼砂〔毫升〕pH0.05M硼砂〔毫升〕0.2M硼酸〔毫升〕7.47.67.88.01.0

1.52.03.09.08.58.07.08.28.48.79.03.54.56.08.06.55.54.02.0硼砂Na2B4O7·H2O,分子量=381.43;0.05M溶液〔=0.2M硼酸根〕含19.07克/升。硼酸H2BO3,分子量=61.84,0.2M溶液为12.37克/升。硼砂易失去结晶水，必须在带塞的瓶中保存。11．硼酸-硼砂缓冲液〔0.2M硼酸根〕pH0.05M硼砂〔毫升〕0.2M硼酸〔毫升〕pH0.05M硼砂〔毫升〕0.2M硼酸〔毫升〕7.47.67.88.01.01.52.03.09.0

8.58.07.08.28.48.79.03.54.56.08.06.55.54.0

2.0硼砂Na2B4O7·10H2O,分子量=381.43;0.05M溶液〔=0.2M硼酸根〕含19.07克/升。硼酸H2BO3，分子量=61.84,0.2M溶液为12.37克/升。硼砂易失去结晶水，必须在带塞的瓶中保存。12．甘氨酸–氢氧化钠缓冲液〔0.05M〕X毫升0.2M甘氨酸+Y毫升0.2NnaOH加水稀释至200毫升pHXYpHXY8.68.89.09.29.450505050504.06.08.812.016.89.69.810.010.410.6505050505022.427.232.038.645.5甘氨酸分子量=75.07;0.2M溶液含15.01克/升。13．硼砂-氢氧化钠缓冲液〔0.05M硼酸根〕X毫升0.05M硼砂+Y毫升0.2NNaOH加水稀释至200毫升pHXYpHXY9.39.49.65050506.0

11.023.09.810.010.150505034.043.046.0硼砂Na2B4O7·10H2O,分子量=381.43;0.05M溶液为19.07克/升。14．碳酸钠-碳酸氢钠缓冲液〔0.1M〕Ca2+、Mg2+存在时不得使用pH0.1MNa2CO3(毫升)0.1MN2HCO3(毫升)20379.169.409.519.789.9