甘蓝型油菜低温诱导的转录组和蛋白组分析

importantoilseedcrop,theBrassicanapusisofgreatsignificanceforstabilizingrapeseedyield.Inrecentyears,withthedevelopmentofomicsbecomeimportantmeanstorevealtheresponsemechanismsofBrassicanapusastheresearchtechniques,aimingtocomprehensivelyanaresponsemechanismofBrassicanapusunderlow-temperatureofcoldtoleranceinrapeseed.Inthisstudy,wefirsaftertreatment.Subsequently,acomprehensiveanalysisofthetranscriptomeandproteomeofBrassicanapusunderlowtemperaturestresswassequencingandmassspectrometrytechniquelevelsofBrassicanapusufurtherexplorethefunctionsandmechanismsofthesekeymoleculesinthecoldtoleranceofBrassicannewideasandmethodsforgeneticThisarticleaimstocomprehensivelyreveresponsemechanismofBrassicanapusunderlowtemperatstressthroughtranscriptomeandproteimprovementofcoldtoleranceinrapeseed.二、材料与方法selectedBrassicanapusL.astheexperimentalmaterienvironment,wellgrowintoinductiontreaSamplesweretakensubsequenttranscriptomeandthereferencegenomeusingCufflinksoftware.PerformProteinextractionandmassseparatedbySDSelectrophoresis.SelectproteinbandsformassspectrometryanalysistooProteomicdataanalysis:ComparetheproteinseobtainedfrommassspectrometryanalysiswofBrassicanapusproteinstodeterminetheiridentity.UseRBioinformaticsanalysis:Combiningtranscriptomeaproteomicdata,conductin-depthanalysisonthemolecularresponsemechanismofBrassicanapusunderlowtemperaturestress.Usingbioinformatgenesandproteins,inordertorevealtheirinvolvementintranscriptomeandproteomedata,thisstudyselectWesternBlotresultswithRNAseqandanalyzedthetranscriptomeandproteomechangesofBnapusunderlowtemperaturestress,providingstrongsupportforadeeperunderstandandinducedbiologicalreactionsthroughlow-temptreatment.Subsequently,transcriptometechnologyandmassspectrometryanalysis,wehofgenesthatundergosignificantchangesundbiologicalfunctions,includingsignaltransmetabolicprocesses,transcriptionalregulation,etc.Amonginthelow-temperatureadapexpressionlevelsofmanyptreatment.Theseproteinsinvolvevarisuchasenergymetabolism,proteinsynthesis,cellimportanceoftheseproteinsinthelow-tempmechanismofBrassicanapus.expressionpatternsandabundancechangesundlow-temperatureinduction.ThesegeneofBrassicanapus,whichisthefocusofoursubsequentthroughtranscriptomeandproteomicanalysis.Theseresultsreferencesforthecoldanalyzeditstranscriptomeandproteomettranscriptomeanalysis,weidentifiedaseriesofdifferentiallyexpressedgenestress,whichmayplayanimportantroleintheresistanceofC-repeat/DRE顺式作用元件上，进而激活一系列下游冷响应基因的crucialregulatoryroleinplantresistancetolowtemperaturestress.TheCBF/DREB1transcripexpressionofaseriesofdownstreamcoldresponsivegenes.deeperunderstandingofthelow-temperaturestressresponsemechanisminBrassicanapus.resistanceofBrassicanapustolowtemperaturestress.Antioxidantenzymescanelreduceoxidativedamage;Iontransportproteinscionbalancebothinsideandimbalancedosmoticpressure;EproteinscanprovidesufficientenergysunormalgrowthanddevelopmentofBraWealsodiscoveredsomeunknowngenesandresourcesforBrassicanapustoresistlowtemperaturestreswhichisofgreatsignificanceforfurtherrresponsemechanismofBrassicanapustolowtemperaturestress.ThisstudyrevealedpartistressresponseinBrassicanapusunderstandingofthemolecularmechanismsofBrassicanapusinresistinglowtemprevealthemolecularnetworkoflowtemperaturestressresponsecomplexmechanismsoflowtempBrassicanapusbyconstructinggeneinteractionnetwortechnicalsupportforcoldresistantbreedingandproductionmanagementofBrassicanapusunderlowtresultsindicatethatthetranscriBrassicanapusundergosignificantchangesunderlowtemperaturestress,involvmechanismofBrassicanapusunderlowtemperaturestress.fdifferentiallyexpreunderstandthemolecularregulatorynetworkofBrassicanapusunderlowtemperatuproteinexpressionprofilesbeforeandafterlowtemperaturetreatment.Theseproteincellstructuremaintenance,indexpressionofthesekeyproteinsunderlowtemperatureBasedontheanalysisfoundthatthemolecularresponsemechanismofBrassicanapusunderstandingofthecoldtbutalsoprovidesatheoreticalbasisandcandidatresourcesforimprovingthe性具有重要的理论和实践意义。ThisstudyrevealedthemoleculinformationandidtoleranceofBrassicanapus.Theseresultshaveimportantmolecularmechanismsofplantcoldtoleranceandimprovingthecoldtoleranceofrapeseed.在完成这篇《甘蓝型油菜低温诱导