用流式细胞仪分析脂肪细胞文档

用流式细胞仪分析脂肪细胞聪明出于勤奋，天才在于积累AnalysisandIsolationofAdipocytesbyFlowCytometryang2016-0405MajkaSM,MillerHL,HelmKM,etal.AnalysisandIsolationofAdipocytesbyFlowCytometryMethodsinenzymology2014537:281296.d01:10.101689780-12411619-100015×CONTENTSBackgroundAimoftheresearchDifficultSolutionBackoundWhatareadipocytes(fatcells)?MajorcellsintheadiposetissuThestructureofadiposetissuedeeptotheskinLMX340BackoundTwokindsofadipocytesinhumanLipiddroplethitefatcellBrownFatCellBackoundProspectsofadipocytebiologyLipidstoreLeptinInhibiteatingIncreasemetabolismBackoundProspectsofadipocytebiologyIAdiponectinMCP-lVisfatinipidstoreAdipokinesInvolvedinenergymetabolismBackoundProspectsofadipocytebiologySpecificallyfromadiposetissueIAdiponectinMCP-lVisfatinBloodvesseipidstoreEnergymetabolismBackounProspectsofadipocytebiologyAdipokinesplayansignificantroleinbiologicalfunctionProteinsinvolvedin(e.g,plasminogenactivatoRegulatorsofbloodnhibitor-1,tissuefactor)Hormone-likeproteins(e.g,leptinandADIPOKINESAcute-phaseClassicalcytokines(e.g,C-reaeptide)Promotersofangiogenesis(e.g,vascularendotheliagrowthfactor)BackoundPrinciplediagramDetectorFlowcytometry(FCM)BackoundVolumeDetectorMorphologicalcomplexityofcells,TotalDNA/RNAcontentApoptosisCellviability.10谢谢你的阅读知识就是财富丰富你的人生71、既然我已经踏上这条道路，那么，任何东西都不应妨碍我沿着这条路走下去。——康德

72、家庭成为快乐的种子在外也不致成为障碍物但在旅行之际却是夜间的伴侣。——西塞罗

73、