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14年整体签约情况:160人左右通过审核,主要是银牌与部分铜牌及省一;72其余人获得降40分录取。15年规模预估: 生科院招生规模约为90人左右,今年国赛现场金牌已保送30,其中有10多人将去医学 共约50人左右已入坑。综合考虑到 年留给生物营降分的人数应在20人左右。审核方面,对于强省省一,也将有更 同学能参与选拔过程,预计过审人数仍为100+。面试每年略有不同,大致包括自我介绍、英文专业文章阅读以及高中生活相生物化学-589nm;解析蛋白结构用什么硫氨酸)可通过S-腺苷甲硫氨酸(SAM)提供“活性甲基”(一碳单位)(CDK 工程相关20题左右,如:如何将DNA导入酵母细胞;各类限制酶的1
面试共分六组,每组三位教授;一一进行单面。面试内容既包括生物专业问题,也涉及学生生活的方方面面。前一部分请关注生物科技方面的与(如新鲜出炉的诺贝尔奖),后面一部分则可以比对参照的自主招生面试试题。 (GoldenRice)。 该课程为针对金秋营的模拟面试培训,欢迎市获得生物联赛一等奖以上奖项,元(含建档费外地学员需先建档方可报名。现场将笔试、面试相关模拟题与真回忆与自招面试等。登陆http 本科生考试试题纸 考试课 Biochemistry 2005 1月 说明:1.1-402分,答案可能是一个或多个,请将本卷满分为100Whichcompoundlinksglycolysis,nucleotidesynthesis,andglycogenglucose6-glycerol3- Youhavediscoveredacompoundthatinhibitsfumarase.HowmanymolesofATPwouldyouexpecttobegeneratedfromonemoleofacetyl-CoAinthepresenceofthis56NoATPwouldformunderthese IndicatewhichofthefollowingeventscanoccurduringtheprocessingofeukaryoticmRNAtranscripts.Attaentofapoly(A)tailtothe5’endoftheMethylationofallGExcisionsofConversionofstandardbasestomodifiedbasessuchasSplicingtogetherofDifferentialcuttingandsplicingtoproducetwodifferent AlthoughDNAreplicationhasveryhighfidelity,mutationsdooccur.Whichofthefollowingtypesofsinglebase-pairmutationswouldbemostlikelytobealethalBand WhichofthefollowingcorrectlydescribesthenitrogenFixationofatmosphericnitrogenbynitrogen-fixingbacteriayieldsNitrateisreducedtoammoniainaprocessknownasBiologicalnitrogenfixationiscarriedoutbyacomplexofproteinscallthenitrogenasecomplex.AandA,B,and Assumingthe5’ 3’connectionofwritingnucleotidesequence,indicatewhichofthefollowingmRNAcodonscanberecognizedbythetRNAanticodonICG. Both binationandtransposon- occurbetweenhomologousregionsonareimportantintherepairofdamagedinvolveRecAgenerategeneticThetwotypes binationhavenothingin ConsiderthemRNAsequence:(5’)AAUGCAGCUUUAGCA(3’).ThesequenceofthecodingstrandofDNAis: Thesynthesisofpalmitate8acetyl-147AandA,B,and Enoyl-CoAisomeraseisneededforthecomplete-oxidationunsaturatedfattyacidswithcisdoubleodd-numberfattysaturatedfattyunsaturatedfattyacidswithtransdouble WhichofthefollowingisacharacteristicofmanyaminotransferaseTheyhavealarge,negativeTheaminogroupistransferredtoan -ketoacid(suchas-ketoglutarate)toformthecorrespondingaminoacid.TheaminogroupistransferredfromanammoniaTheyarecatalyzedbythesameTheyrequirethecofactorS- IftheG’ofthereactionABis–12kJ/mol,whichofthefollowingstatementsarecorrect?(NotetheprimesymbolmeansthatathermodynamicparameterismeasuredatpH7.0)ThereactionwillproceedspontaneouslyfromlefttorightatthegivenThereactionwillproceedspontaneouslyfromrighttoleftatstandardTheequilibriumconstantfavorstheformationofBovertheformationofTheequilibriumconstantcouldbecalculatediftheinitialconcentrationsofAandBwereknown.ThevalueofG’oisalso WhichofthefollowingisnotaphysiologicalroleofallostericintermediatesforbiosyntheticcomponentsofmanycomponentsofthecoenzymesNAD,FAD,andintracellularsignaling IndicatewhichofthefollowingstatementsaboutthelacoperonisAllsevengenesaretranscribedintomRNA,whichisthentranslatedintofivedifferentproteins.Therepressorbindstothestructuralgenes,preventingtheirEfficientbindingofRNApolymerasetoDNAoccursonlyinthepresenceofCRPandcAMP.cAMPactsasaninducerbybindingtotheLacIntheabsenceoflactose,therepressorbindstheAmutationintheIgenesuchthatnogeneproductismadeleadstoconstitutiveexpressionoftheZ,Y,andAgenes. Whichpaircorrectlymatchestheenzymewithitsallostericpyruvatekinase;pyruvatedehydrogenase:pyruvatecarboxylase; WhichofthefollowingdescribesfattyacidtransportintothemitochondrialItistherate-limitingstepinfattyacidItisregulatedbymalonyl-ThecytosolicandmatrixpoolsofCoAaredistinctandOncefattyacylgroupshaveenteredthematrix,theyarecommittedtooxidationtoAlloftheaboveare Innucleotidemetabolism,allofthefollowingaretrueThecommitmentstepinpurinebiosynthesisisthetransferofaminogrouptoBothpurineandpyrimidinebiosynthesisareregulatedbyend-productNucleotidescanbesynthesizedinasinglereactionviasalvageDenovopyrimidinesynthesisbeginswithamoleculeofOrotidylateisthecommonprecursorinthebiosynthesisofpyrimidines,andinosinateisthecommonprecursorinthebiosynthesisofthepurinesATPandGTP. Themostefficientwaytoturnoffglycogendegradationisdecreasetheactivityofphosphorylaseincreasetheactivityof increasetheactivityofincreasetheactivityofglycogendecreasetheintracellularlevelsof Insomerespects,theureacycleandthecitricacidcycleareogousprocesses.Ornithineandcitrullinehaverolesthataresimilartothoseofoxaloacetateandwhatothercitricacidcycleintermediate? Indicatewhichofthefollowingstatementsaboutthemitochondrialelectrontransferandoxidativephosphorylationaretrue.SynthesizedATPmustbetransportedintotheintermembranespacebeforeitcanenterthecytosol.CytochromecandtheF1subunitofATPaseareperipheralmembraneComplexesI,II,IIIandIVareallprotonUbiquinoneisahydrophilicThefinalelectronacceptoris Whichofthefollowingdescribestheactivityandregulationofribonucleotide eand/oritsimportancetothecell?Bothofitsactivityanditssubstratespecificityareregulatedbythebindingofeffectormolecules.ATPincreasestheoverallactivityoftheControloftheenzyme’sactivityensuresabalancedpoolofprecursorsforDNABalancedpoolsofdeoxyribonucleotidesarenecessaryinDNAsynthesis,giventhecomplementarybase-pairingofnucleotidesindouble-strandedDNA.Alloftheaboveare Earlierinitslife,atadpolelivesinanaqueousenvironmentandexcretesmuchofitsexcessnitrogenasammonia.Onceitmaturesintoanadult,thefrogspendsmuchtimeondrylandand esureoic.Whichofthefollowingenzymeactivitieswouldbemostlikelytoincreasedrasticallyintheadultfrog?carbamoylphosphatesynthetaseglutamine-ketoglutarateAnswer(s):ThediscontinuityofeukaryoticgeneswasfirstrevealedusingfootprintingRNAsequenceelectron ysisofRNA-DNAhybridntgenetic AmesTestisusedtoinvestigatewhetherachemicalreagent Asampleofmalonyl-CoAsynthesizedfromradioactive(14Clabeled)HCO3-andunlabeledacetyl-CoAisusedinfattyacidsynthesis.Inwhichcarbon(s)willthefinalfattyacidbelabeled?(RecallthatthecarboxylcarbonisC-1.)everyeveryodd-numberedeveryeven-numberedonlythecarbonfarthestfromC-nopartofthemoleculewillbe NottakingintoaccounttheNADHgeneratedinthemalatedehydrogenasereaction,howmanyhigh-energyphosphatebondsareusedtoformamoleculeofurea,startingfromammoniaandHCO3-?A.B.C.D.E. DNApolymeraseI 5’exonuclease 3’exonucleaseproofreadingpolymerase Theproton-motivegeneratedbytheelectrontransferincludesapH-gradientincludesanelectrical-potentialisusedforactivetransportisusedtosynthesizehasalloftheabove Whichstatementisnottrueofphosphofructokinase-Itisinhibitedbyfructose2,6-ItisactivatedbyItisinhibitedbyItisinhibitedbyATPincreasesitsK0.5forfructose6- SomephotosyntheticprokaryotesuseH2S,hydrogensulfide,insteadofwaterastheirphotosynthetichydrogendonor.HowdoesthischangetheultimateproductsofCarbohydrate(CH2O)isnotH2OisnotOxygenisnotTheproductsdonotAnswer(s):Whichofthefollowinganswerscompletethesentencecorrectly?Surplusdietaryaminoacidsmaybeconvertedintoketoneavarietyofbiomoleculesforwhichtheyare Humanchromosomesareextremelylarge,complexstructures.Whichofthefollowingstatementscorrectlydescribestheorganizationofthehumanchromosomes?Allofthegeneticinformationofthecellisencodedinthenuclear,chromosomalGenesforhistonesandribosomalRNAsarestructuralMostofthechromosomalDNAcodesforAandA,B,and MethylationofGATCsequencesatfrequenciesinE.colicells.TheleadingThelagging
isbelievedtocontrolthecausesprematureterminationofpolypeptidesynthesisbymimickingaminoacyltransferRNA:itisabletoentertheAsiteandacceptthepeptidylgrouptoformapeptidylmolecule,whichhoweverwilldissociatefromtheribosome. WhichofthefollowingcompoundsdonotdirectlyprovideatomstoformthepurineCarbamoyl4NH4 WhichofthefollowingistrueofthecontrolofGlucagonstimulatesadenylylcyclase,causingtheformationofCyclicAMPstimulatesthephosphorylationandthusincreasestheactivityofFBPase-2activitylowerstheleveloffructose-2,6-bisphosphate,thusincreasingtherateofgluconeogenesis.ItisreciprocallylinkedtothecontrolofAlloftheaboveare CarbonfixationinvolvesacondensationreactionbetweenCO2ribulose1,5-fructose6-ribose5- HowdothehormonesepinephrineandglucagondifferintheirmetabolismofOnlyepinephrinestimulatesOnlyglucagonstimulatesOnlyepinephrineactsonOnlyglucagonstimulates Theenzyme(s)responsibleforthetranscriptionofeukaryoticrRNARNApolymeraseIandRNApolymeraseIIand Theenzymaticprocessof“charging”amoleculeoftRNAissimilartootherenzymecatalyzedreactionsyouhaveencounteredinthiscourse.Whichofthefollowingcorrectlydescribessuchsimilarities?Formationofanacyl-adenylateintermediatecoupledtothehydrolysisofpyrophosphateissimilartoreactionsinvolvedinfattyacidactivation.ProofreadingandcorrectingabilitiesthatpreventincorporationofthewrongmoleculeareogoustotheactionofDNApolymerase.Theaminoacyl-tRNAsynthetaseisarelativelynonspecificenzyme(i.e.itcanactivatemanydifferentaminoacids)andintherespectissimilartoribonucleotideredue.AandAandBand Exinwhyskeletalmuscledoesnotcontributeglucosetotheblood.(4points)Answer:Becauseitlackstheenzymeglucose6-phosphatase.Exinwhyuntreateddiabeticsloseweight.(4Answer:Therateoftriacylglycerolbiosynthesisisaffectedbytheactionofseveralhormones,oneofwhichisinsulin.Insulinpromotestheconversionofcarbohydratestotriacylglycerols.Peoplewithseverediabetes,duetofailureofinsulinsecretionoraction,notonlyareunabletouseglucoseproperlybutalsofailtosynthesizefattyacidsfromcarbohydratesoraminoacids.Untreateddiabeticshaveincreasedrateoffatoxidationandketonebodyformation,asaconsequence,loseweight.SupposethatabacterialmutantisfoundtoreplicateitsDNAataverylowrate.Uponysis,itisfoundtohaveentirelynormalactivityofDNApolymerasesIandIII,DNAgyrase,andDNAligase.ItalsomakesnormalamountsandkindsofdnaA,dnaB,dnaC,andSSBproteins.TheoriCregionofitschromosomeisfoundtobeentirelynormalwithrespecttonucleotidesequence.WhatdefectmightaccountfortheabnormallylowofDNAreplicationinthismutant?Exin.(4Answer:AdecreaseintheactivityofprimasewouldaccountforthelowrateofDNAreplication.DNAreplicationrequiresthepriorsynthesisofRNAprimers.DecreasedratesofdNTPsynthesiswouldalsoslowreplication.DescribethreepropertiescommontothereactionscatalyzedbyDNApolymerase,RNApolymerase,andreversetranscriptase.Howistheenzymepolynucleotidephosphorylasedifferentfromtheseenzymes?(8points)Answer:theseenzymeshaveatleastfourpropertiesinAllare tedirected,synthesizingasequencecomplementarytothe Synthesisoccursina5’to3’AllcatalyzetheadditionofanucleotidebytheformationofaphosphodiesterAlluse(deoxy)ribonucleosidetriphosphatesassubstrate,andreleasepyrophosphateasaproduct.Theenzymepolynucleotidephosphorylasediffersfromtheseenzymesinpoints1and4.Itdoesnotuseatemte,butratheraddribonucleotidestoanRNAinahighlyreversiblereaction.Thesubstrates(inthedirectionofsynthesis)areribonucleosidediphosphates,whichareaddedwiththereleaseofphosphateasaproduct.Inthecell,thisenzymeprobablycatalyzesthereversereactiontodegradeRNAs.本科生考试试 考试课程Biochemistry说明:1.(1-30)或写在答题纸上(31-33IftheG`ofthereactionABis–12kJ/mol,whichofthefollowingstatementsarecorrect?(NotetheprimesymbolmeansthatathermodynamicparameterismeasuredatpH.7.0)(2points)ThereactionwillproceedspontaneouslyfromlefttorightatthegivenThereactionwillproceedspontaneouslyfromrighttoleftatstandardTheequilibriumconstantfavorstheformationofBovertheformationofTheequilibriumconstantcouldbecalculatediftheinitialconcentrationsofAandBwereThevalueofG`oisalsoWhichofthefollowingstatementsaboutATPanditsrolesincellsaretrue?(2TheATPmoleculeiskineticallyunstableandisthusconsumedwithinaboutoneminutefollowingitsformationincells.ATPprovides energytoathermodynamicallyunfavorablereactionsbygrouptransfer,alwaysdonatingaPitoformacovalentintermediate.ATPcanberegeneratedbycouplingwithareactionthatreleasesmore energythandoesATPhydrolysis. candriveATPTheactiveformofATPisusuallyinacomplexwithAnswer(s):C,D,AcommonmoietyforNADP,NAD,FMN,FAD,andcoenzymeAis:(2ApyrimidineAthreeringApyranoseAtriphosphateIftheC-1carbonofglucosewerelabeledwith14C,whichofthecarbonatomsinpyruvatewouldbelabeledafterglycolysis?(2points)ThecarboxylateThecarbonylThemethylWhichofthefollowingaremetabolicproductsofpyruvateinhigherorganisms?(2LacticIndicatewhethereachofthefollowingstatementsaboutthepentosephosphatepathwayistrue(T)orfalse(F).(5points)ItgeneratesNADHforreductivebiosyntheses.Thereactionsoccurinthecytosol.Transketolaseandtransaldolaselinkthispathwaytogluconeogenesis.Itismoreactiveinmusclecellsthaninfat-storagecells.Itinterconvertstrioses,tetroses,pentoses,hexoses,andheptoses.Whichofthefollowingstatementsarecorrect?Thecitricacidcycle(2doesnotexistassuchinntsandbacteria,becauseitsfunctionsareperformedbytheglyoxylatecycle.oxidizesacetylCoAderivedfromfattyacidproducesmostoftheCO2inanaerobicprovidessuccinylCoAforthesynthesisofprovidesprecursorsforthesynthesisofglutamicandasparticMatchthecofactorsofthepyruvatedehydrogenasecomplexwiththeircorrespondingenzymecomponentsandwiththeirrolesintheenzymaticstepsthatarelisted.(5points)CoenzymeA:NAD+:2,Thiaminepyrophosphate(TPP):1,Lipoamide:3,OxidizesthehydroxylethylAcceptstheacetylgroupfromProvidesalong,flexiblearmthatcoveysintermediatestodifferentenzymeOxidizesMatchingtheroleinfattyacidoxidationand/ormobilizationtotheappropriatecomponentlistedbelow.(5points)Bilesalt:Serum Actsasa“carrier”offattyacidsacrosstheinnermitochondrialActsasabiologicaldetergent,disruptingfatglobulesintosmallmixedBindsandtransportstriacylglycerols,phospholipids,andcholesterolbetweenBindssomefattyacidsmolibizedfromadipocytesandtransportstheminthebloodtoheartandskeletalmuscle.Whichofthefollowinganswerscompletethesentencecorrectly?Surplusdietaryaminoacidsmaybeconvertedinto(2points)ketoneavarietyofbiomoleculesforwhichtheyareAnswer(s):A,B,C,Whichofthefollowingcompoundsservesasanacceptorfortheaminogroupsofmanyaminoacidsduringmetabolism?(2points)Matchthefunctionsforthecoenzymesthatareinvolvedinaminoacidmetabolism.(4Pyridoxalphosphate:CoenzymeB12: NAD+: BiotinCarriesCarriesaminoWhichofthefollowingexperimentalobservationswouldnotsupportthechemiosmoticmodelofoxidativephosphorylation?(3points)Ifmitochondrialmembranesareruptured,oxidativephosphorylationcannotRaisingthepHofthefluidintheintermembranespaceresultsinATPsynthesisintheTransferofelectronsthroughtherespiratorychainresultsinformationofaprotongradientacrosstheinnermitochondrialmembrane.TheorientationoftheenzymecomplexesoftheelectrontransferchainresultsinaunidirectionalflowofH+.RadioactivelylabeledinorganicphosphateisincorporatedintocytosolicATPonlyinthepresenceofanH+gradientacrosstheinnermitochondrialmembrane.SomephotosyntheticprokaryotesuseH2S,hydrogensulfide,insteadofwaterastheirphotosynthetichydrogendonor.Howdoesthischangetheultimateproductsofphotosynthesis?(2Carbohydrate(CH2O)isnotH2OisnotOxygenisnotTheproductsdonotWhichofthefollowingareconstituentsofchlorophylls?(2SubstitutedIron Theobservationthattheincubationofphotosyntheticalgaewith14CO2inthelightforaverybrieftime(5s)ledtotheformationof14C-labeled3-phosphoglyceratesuggestedthatthe14CO2wascondensingwithsometwo-carbonacceptor.Thatacceptorwasinfactwhichofthefollowing?(2AcetylAcetylGlycerolNoneoftheWhichofthefollowingarecommonfeaturesofthesynthesesofmevalonate(anintermediateofcholesterolbiosynthesis)andketonebodies?(2points)BothrequireBothrequiretheHMG-CoAcleavageBothoccurintheBothoccurinliverS-adenosylmethionineisinvolveddirectlyinwhichofthefollowingreactions.(3MethyltransfertophosphatidylSynthesisofglycinefromSynthesisofConversionofhomocysteinetoGenerationofthe5`capoftheeukaryoticWhichofthefollowingdoesnotprovideacarbonskeletonforthesynthesisofaminoacids?(2Whichofthefollowingcompoundsdirectlyprovideatomstoformthepurinering?(3Carbamoyl4NH4Answer(s):A,C,D,E,Whichofthefollowingstatementsaboutribonucleotide earetrue?(2Itconvertsribonucleosidediphosphatesinto2`-deoxyribonucleosidediphosphatesinItcontainscoenzymeB12,whichgeneratesradicalsneededfortheItacceptselectronsdirectlyfromItreceiveselectronsdirectlyfromeitherthioredoxinorItcontainstwokindsofallostericregulatorysites:oneforcontrollingtheoverallactivityandtheotherforcontrollingthesubstratespecificity.Answer(s):A,D, BiosyntheticpathwaysthatrequireNADPHincludewhichofthefollowing?(2FattyacidKetonebodyCholesterol binationinE.coliislikelytorequirewhichofthefollowing?(3DnaBRecARecBCDSingle-strandDNAbindingAnswer(s):B,C,D,E,F,H,I,WhichofthefollowingstatementsaboutE.colipromotersarecorrect?(2TheymayexhibitdifferenttranscriptionFormostgenestheyincludevariantsofconsensusTheyspecifythestartsitesfortranscriptionontheDNAtemTheyhaveidenticalanddefiningTheyareactivatedwhenCorGresiduesaresubstitutedintotheir–10regionsbyThosethathavesequencesthatcorrespondcloselytotheconsensussequencesandareseparatedby17basepairsareveryefficient.Answer(s):A,B,C,TheAAUAAAsequenceonaRNAmoleculemarks(2ThesitewhereribosomesbindtoinitiatepolypeptideThesitewheretranscriptionThesitenearwhichtheprimarytranscriptiscleavedandapoly(A)sequenceisThesitewherethereleasefactorwillbindtoendpolypeptideThesitewherepolyribonucleotidephosphorylasewilladdastretchofrandomThe70subunitoftheE.coliRNApolymerase:(2actsasthecatalyticsiteforRecognizeHasaproofreadingIncreasestheprocessivityoftheRecognizesterminationThediscontinuityofeukaryoticgeneswerefirstrevealedby:(2UsingfootprintingRNAsequenceElectronmicroscopicysisofRNA-DNAhybridntgeneticWhichofthefollowingmRNAcodonscanberecognizedbythetRNAanticodonICG.(2Answer(s):B,D,Anewcompound,vivekine,wasrecentlydiscoveredbyacleverundergraduatestudent.Itwasisolatedfrombacteriafoundindeepsea-dwellingorganisms.Vivekineinhibitsproteinsynthesisineukaryotes:Proteinsynthesiscaninitiate,butonlydipeptidesareformedandtheseremainboundtotheribosome.Thistoxinaffectseukaryoticproteinsynthesisbyblockingthe:(2points)bindingofformylmethionyl-tRNAtoactivityofelongationactivationofaminorecognitionofstopformationofpeptideIndicatewhethereachofthefollowingstatementsaboutprokaryotictranslationistrue(T)or(7Anaminoacyl-tRNAsynthetasecatalyzesformationofanesterbond.( AnmRNAmoleculecannotbeusedtodirectproteinsynthesisuntilithasbeencomple transcribed.( ThepositioningoffMet-tRNAontheAsitedefinesthereadingframe. ingaminoacyl-tRNAarefirstboundtotheAsite. Formationofthe70Sinitiationcomplexrequiresaninputofenergy. Thecarboxylgroupoftheaminoacidontheaminoacyl-tRNAistransferredtotheaminogroupofapeptidyl-tRNA.( ReleasefactorscausethepeptidyltransferaseactivityoftheribosometouseH2Oasasubstrate.( InanattempttodeterminewhetheragivenRNAwascatalyticallyactiveinthecleavageofasyntheticoligonucleotide,thefollowingexperimentalresultswereobtained.WhentheRNAandtheoligonucleotidewereincubatedtogether,cleavageoftheoligonucleotideoccurred.WheneithertheRNAortheoligonucleotidewasincubatedalone,therewasnocleavage.WhentheRNAwasincubatedwithhigherconcentrationsoftheoligonucleotide,saturationkineticsoftheMichaelis-Mententypewereobserved.DotheseresultsdemonstratethattheRNAhascatalyticactivity?Exin.(8points)Answer:TheseresultsalonedonotestablishthattheRNAhascatalyticactivity.Acatalystmustberegenerated.Itisentirelypossiblethattheresultsobservedcouldbeaccountedforbyastoichiometric,asopposedtoacatalytic,inctionbetweenRNAandtheoligonucleotide.InwhichtheRNAmay“commitsuicide”astheoligonucleotideiscleaved.Insuchaninction,aportionoftheRNAwouldalsobecleaveditselfasapartofthereaction.Fourreactionproductswouldaccumulate,tworesultingfromthecleavageofRNAandtwofromthecleavageoftheoligonucleotide.ToshowthatthisparticularRNAwascatalytic,itwouldbenecessarytodemonstratethatitturnsoverandisregeneratedinthecourseofthereaction.Translationinvolvesconversionofthelanguageofnucleotidestothatofproteins.InthechainofeventsleadingfromanucleotidesequenceonDNAtotheproductionofproteinbyribosomes,wherepreciselydoestheprocessoftranslationoccur?Exin.(6points)Answer:Translationinvolvesconversionofthelanguageofnucleotidestothatofproteins.Theagentoftranslationistheappropriateaminoacyl-tRNAsynthetase,whichmustrecognizeaparticularaminoacidandlinkittoatRNAcontainingananticodonforthataminoacid.SupposethatabacterialmutantisfoundtoreplicateitsDNAataverylowrate.Uponysis,itisfoundtohaveentirelynormalactivityofDNApolymerasesIandIII,DNAgyrase,andDNAligase.ItalsomakesnormalamountsandkindsofdnaA,dnaB,dnaC,andSSBproteins.TheoriCregionofitschromosomeisfoundtobee
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