草鱼幼鱼蛋氨酸需要量(王胜)

L-MethionineRequirement

ofJuvenileGrassCarp

草鱼幼鱼蛋氨酸需要量WangSheng（王胜）LiuYong-jian（刘永坚）TianLi-xia（田丽霞）*XieMing-quan（谢明权）YangHui-jun（阳会军）LiangGui-ying(梁桂英)DuZhen-yu(杜震宇)WangJi-teng(王骥腾)InstituteofAquaticEconomicAnimals,SunYat-senUniversity中山大学水生经济动物研究所

*

DepartmentofScienceandTechnologyofGuangdong*广东省科技厅PopularfreshwaterNo.2China,allovertheworld

Littlereportonquantitativenutrientrequirementininternationaljournalsexceptforprotein&energyIntroduction

Methioninerequirement(baseongrowthstudies)variesamongspecies---Arcticcharr(lowest)---Commoncarp---Rainbowtrout---Giltheadseabream(highest)Rang:1.8-4.0(aspercentofprotein)(NRC,1993)

TwoMainFactorsAffectingRequirements

SpeciesMethodology:

Criterion:Growthstudies;Proteinaccretion;Idealprotein;A/Eratios;Lenspathology;SerumorTissueAminoAcidStudies;AminoAcidOxidationStudiesb.FishSize,Agec.Dietformulation:

DE,ADCofprotein,cystinesparing,chloride,VitA,leachingd.

Rearingcondition:Watertemperature

e.Modelused:brokenline<othersObjectivesTodeterminethedietarymethioninerequirementofjuvenilegrasscarpMaterials&MethodsPHofdiet:7.0-7.2Precoated(CMC,Carrageenan)ADC:Y2O3Feedingrate:2.5%bodyweightperday

Replications:3tanks/diet,20fish/tank(12.5±0.3g)Feeding:hand-fed6timesdailyPeriod:10wksWatertemp:25.5±2.5

°CFormulationofDiets*CorrespondingtotheaminoacidpatternfoundinthemuscleproteinofgrasscarpexceptformethionineIngredientsTestdiets(gkg－1drydiet)*12345678L-Methionine0.01.73.45.06.78.410.111.8Asparticacid:Glycine(1:1)

11.810.18.46.75.13.41.70.0Others988.2988.2988.2988.2988.2988.2988.2988.2Others(g.kg-1diet):Casein,111;

Gelatin,111;Aminoacidmixture,138.3;Fishoil:Cornoil(1:1),60.0;Dextrine,200;Ascorbyl-2-monophosphate,10;Cholinechloride(50%),8;Cellulose,184.9;Vitaminmix,20;Mineralmix,80.0;Carboxymethycellulose(CMC),40;K-Carrageenan,25;Y2O3,0.1.

Table2Aminoacidcomposition(gper100gdrydiet)ofexperimentaldietsformethioninerequirementstudy——0.3980.000Hydroxyproline0.407—0.01130.0253Cystine1.1310.4580.0680.605Tyrosine2.4161.0421.0130.361Alanine2.7710.0102.5310.230Glycine2.6391.2250.8240.590Proline1.0560.0730.4000.583Serine5.4852.0091.1402.006Glutamicacid3.6151.9440.6050.736AsparticacidNon-essentialanimoacids1.5820.6920.2810.609Valine0.3540.2380.0010.115Tryptophan1.3960.5480.4280.420Threonine1.3790.6310.2580.490Phenylalanine0.896variable0.0850.268Methionine2.6741.4330.4390.802Lysine22.5061.1390.3950.972Leucine1.3790.6890.1850.505Isoleucine0.9440.5930.0690.282Histidine2.3701.1000.8690.401ArginineEssentialanimoacidseggproteinanimoacidsgelatin(10g)casein(10g)35gmuscleorCrystallineAmountinAmountinAnimoacids1CompositionofDiets(g/kgdrydiet)

L-methionine

3.55.26.98.510.211.913.615.3Cystine0.360.360.360.360.360.360.360.36L-methionine(gkg－1protein)10.014.819.624.429.234.038.843.6Cystine(gkg－1protein)1.031.031.031.031.031.031.031.03DE(kcal/kg)27402740274027402740274027402740Moisture:97.2—115.3Crudeprotein:365.0—382.5Crudelipid:57.9—60.1Ash:94.2—100.7RESULTS

NoMortalityGrowthPerformancesBody&LiverCompositionsADCSpecificGrowthRateSeconddegreepolynomialregression:10.5(28.3)edcdabaababbcFeedEfficiencySeconddegreepolynomialregression:10.3(27.7)cdbababbb%ProteinEfficiencyRatioProteinRetentionShowthesimilartrendsWholeBodyMoisture

%aabaabbaaWholeBodyLipidandProtein%abaaabbbbbcdbcdabcdabcababcddabcdLipidcontentofliver

Y=-1.1916X+12.701,R2=0.96%aaabbcbcdcddADCoftheproteinAverage:92.4%

%ConclusionsMethioninerequirementofjuvenilegrasscarp:10.5g

perkgdiet(baseonSGR)

(28.3gperkgprotein)

10.3g

methionineperkgdiet(baseonFE)

(27.7gmethionine

perkgprotein)ConclusionsMethioninerequirementofjuvenilegrasscarp:10.5g

perkgdiet(baseonSGR)

(28.3gperkgprotein)10.3g

methionineperkgdiet(baseonFE)

(27.7gmethionine

perkgprotein)LeachingofcrystallineaminoacidActualvalue?alittlelowerthanestimatedvalueConclusionsMethioninerequirementofjuvenilegrasscarp:10.5g

perkgdiet(baseonSGR)

(28.3gperkgprotein)10.3g

methionineperkgdiet(baseonFE)

(27.7gmethionine

perkgprotein)LeachingofcrystallineaminoacidActualvalue?alittlelowerthanestimatedvalueAvailability?lowerThankyouDietno.MethionineLevel(g.kg-1)%ofwettissueMoistureProteinLipidAsh13.578.03±1.56a13.42±0.47b5.65±0.86cd2.96±0.19ab25.277.47±1.86a13.42±0.53b5.95±0.97bcd2.85±0.16b36.976.81±1.91ab13.80±1.15ab6.24±0.85abcd3.15±0.18a48.574.88±0.35b14.61±0.52a7.01±0.36abc3.06±0.12ab510.274.79±0.37b14.58±0.287.17±0.56ab3.06±0.05ab611.977.55±1.32a13.31±0.10ab6.06±0.96abcd2.96±0.03ab713.678.41±0.94a13.09±0.86b5.26±0.42d2.87±0.08b815.377.29±1.02a13.15±0.29b6.43±0.88abcd2.86±0.05bProximatecompositionsofwholebody

Dietno.MethionineLevel(