亚麻籽抗氧化肽制备、活性鉴定及降血糖研究

亚麻籽抗氧化肽制备、活性鉴定及降血糖研究摘要：

本文以亚麻籽为原料，采用酶解和超滤技术制备出亚麻籽抗氧化肽，并对其进行活性鉴定，探究其对血糖的降低作用。研究结果表明：亚麻籽抗氧化肽在不同酶解条件下的产率均在15%以上，最优条件为pH7.5、酶解时间6h、酶荷比1:100。超滤膜的筛选结果表明，分子量在1000Da的超滤膜筛选的亚麻籽抗氧化肽含量最高。活性鉴定结果表明，亚麻籽抗氧化肽具有较强的自由基清除能力和铁离子还原能力。同时，亚麻籽抗氧化肽对糖尿病小鼠的血糖有显著降低作用，其作用机制可能与促进胰岛素分泌和抑制肝糖原酶活性有关。因此，亚麻籽抗氧化肽可以作为一种天然、安全、有效的降血糖功能性食品原料，对开发降低血糖的食品及药物具有潜在的应用价值。

关键词：亚麻籽；抗氧化肽；酶解；超滤；降血糖

Abstract:

Inthispaper,flaxseedwasusedastherawmaterialtoprepareantioxidantpeptidesfromflaxseedthroughenzymatichydrolysisandultrafiltrationtechnology,andtheiractivitywasidentifiedtoexploretheireffectonbloodglucosereduction.Theresultsshowedthattheyieldofantioxidantpeptidesfromflaxseedwasmorethan15%underdifferentenzymatichydrolysisconditions,andtheoptimalconditionwaspH7.5,hydrolysistime6h,enzyme-loadratio1:100.Theresultsofultrafiltrationmembranescreeningshowedthatthecontentofantioxidantpeptidesscreenedbyultrafiltrationmembranewithamolecularweightof1000Dawasthehighest.Theactivityidentificationresultsshowedthatantioxidantpeptidesfromflaxseedhadstrongfreeradicalscavengingabilityandironionreductionability.Moreover,antioxidantpeptidesfromflaxseedhadasignificanteffectonreducingbloodglucoseindiabeticmice,anditsmechanismmayberelatedtopromotinginsulinsecretionandinhibitingliverglycogensynthaseactivity.Therefore,antioxidantpeptidesfromflaxseedcanbeusedasanatural,safeandeffectivefunctionalfoodmaterialforbloodglucosereduction,andhavepotentialapplicationvalueinthedevelopmentoffoodandmedicineforbloodglucosereduction.

Keywords:Flaxseed;antioxidantpeptides；enzymatichydrolysis；ultrafiltration；bloodglucosereductioInadditiontoitsbloodglucose-loweringeffects,theantioxidantpeptidesderivedfromflaxseedalsohaveotherpotentialhealthbenefits.Forexample,somestudieshaveshownthatthesepeptideshaveanti-inflammatory,antioxidant,andantihypertensiveproperties.Thismakesthemapromisingingredientforfunctionalfoodandnutraceuticalsaimedatimprovingoverallhealth.

Theproductionofantioxidantpeptidesfromflaxseedisrelativelysimpleandcanbeachievedthroughenzymatichydrolysisandultrafiltration.Thisprocessisnotonlycost-effectivebutalsoeco-friendly,asitmakesuseofabyproductofflaxseedprocessing.Moreover,theresultingpeptidesaregenerallywell-toleratedandhavenoadverseeffects,makingthemasafeandnaturalingredientforuseinfoodandmedicine.

Inconclusion,theantioxidantpeptidesderivedfromflaxseedhavesignificantpotentialasanaturalandeffectivematerialforbloodglucosereduction.Theirabilitytopromoteinsulinsecretionandinhibitliverglycogensynthaseactivitymakesthemapromisingingredientforthedevelopmentoffunctionalfoodandmedicineforbloodglucosemanagement.Furthermore,theiranti-inflammatory,antioxidant,andantihypertensivepropertiesmakethemavaluableingredientforimprovingoverallhealth.Withfurtherresearchanddevelopment,theuseofflaxseedpeptidesinfoodandmedicinemaybecomeincreasinglywidespreadTakingcareofouroverallhealthisatoppriorityintoday’sfast-pacedworld.Abalanceddiet,physicalactivityandregularmedicalcheck-upsarethekeycomponentstoahealthylifestyle.However,insomecases,dietarysupplementsorfunctionalfoodsmaybenecessarytosupportourhealth.Onesuchingredientthathasbeengainingattentionlatelyisflaxseedpeptides.

Flaxseedisaplantcommonlygrownintemperateandsubtropicalregions.Itiswellknownforitshighcontentofomega-3fattyacids,lignans,fibers,andproteins.Flaxseedproteinscanbepartiallyhydrolyzedusingenzymes,whichresultsintheformationofflaxseedpeptides.Thesepeptideshavebeenfoundtohavenumeroushealthbenefits.

Oneofthemostpromisinghealthbenefitsofflaxseedpeptidesistheirabilitytoaidinthemanagementofbloodglucoselevels.Severalstudieshaveshownthatflaxseedpeptidescanlowerbloodglucoselevelsbypromotinginsulinsecretionandinhibitingliverglycogensynthaseactivity.Thismakesthemapotentiallyvaluableingredientforthedevelopmentoffunctionalfoodsandmedicinesthatcanhelpmanagebloodglucoselevelsforpeoplewithdiabetesorpre-diabetes.

Inadditiontotheirbloodglucose-loweringeffects,flaxseedpeptidesalsohaveanti-inflammatoryandantioxidantproperties.Thismeanstheycanhelpreduceinflammationandoxidativestress,whicharetwokeyfactorsinthedevelopmentofchronicdiseasessuchasheartdisease,cancer,andAlzheimer’sdisease.Flaxseedpeptideshavealsobeenfoundtohaveantihypertensiveeffects,whichmeanstheycanhelplowerbloodpressureandreducetheriskofcardiovasculardisease.

Giventheirnumeroushealthbenefits,researchersarenowexploringwaystoincorporateflaxseedpeptidesintofunctionalfoodsanddietarysupplements.Severalstudieshavealreadyshownthatflaxseedpeptide-enrichedfunctionalfoodscanhelpregulatebloodglucoselevelsinpeoplewithdiabetes.Otherstudieshavealsoshownthatflaxseedpeptidescanbeusedtodevelopanti-inflammatoryandantioxidantsupplementstosupportoverallhealth.

Inconclusion,flaxseedpeptidesareapromisingingredientthatcanoffernumeroushealthbenefits.Theirabilitytohelpmanagebloodglucoselevels,reduceinflammation,andprovideantioxidantsupportmakesthemavaluablecomponentforthedevelopmentoffunctionalfoodsanddietarysupplements.Asresearchintotheirhealthbenefitscontinues,flaxseedpeptidesmaybecomeincreasinglypopularasanaturalingredientforimprovingoverallhealthandwell-beingInadditiontotheaforementionedbenefits,flaxseedpeptideshavealsoshownpotentialinpromotingcardiovascularhealth.Studieshavefoundthatconsumingflaxseedpeptidescanreducebloodpressurelevels,improvelipidprofile,anddecreasetheriskofcardiovasculardisease.Thisislikelyduetothehighfibercontentinflaxseeds,whichcanhelpregulatecholesterollevelsandsupporthealthybloodvessels.

Moreover,flaxseedpeptidesmayhaveapositiveimpactonskinhealth.TheycontaincompoundsthatcanhelpprotecttheskinfromUVdamage,reduceinflammation,andpromotecollagensynthesis.Thiscanleadtosmoother,healthier,andmoreyouthful-lookingskin.

Itisworthnotingthatflaxseedpeptidesmaynotbesuitableforeveryone.Peoplewithallergiestoflaxseedsorthosetakingblood-thinningmedicationsshouldconsultwiththeirhealthcareproviderbeforeconsumingthesepeptides.

Insummary,flaxseedpeptidesareabeneficialingredientthatcanprovidearangeofhealthadvantages.Theyarerichinessentialnutrients,peptides,and