BC-1215-DataSheet-生命科学试剂-MedChemExpress

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemEBC-1215Cat.No.:HY-117937CASNo.:1507370-20-8分⼦式:C₂₆H₂₆N₄分⼦量:394.51作⽤靶点:LigandsforE3Ligase作⽤通路:PROTAC储存⽅式:Powder-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性数据体外实验DMSO:12.5mg/mL(31.68mM;Needultrasonic)MassSolvent1mg5mg10mgConcentration制备储备液1mM2.5348mL12.6740mL25.3479mL5mM0.5070mL2.5348mL5.0696mL10mM0.2535mL1.2674mL2.5348mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存⽅式和期限：-80°C,6months;-20°C,1month。-80°C储存时，请在6个⽉内使⽤，-20°C储存时，请在1个⽉内使⽤。体内实验请根据您的实验动物和给药⽅式选择适当的溶解⽅案。以下溶解⽅案都请先按照InVitro⽅式配制澄的储备液，再依次添加助溶剂：(为保证实验结果的可靠性，澄的储备液可以根据储存条件，适当保存；体内实验的⼯作液，建议您现⽤现配，当天使⽤；以下溶剂前显⽰的百分⽐指该溶剂在您配制终溶液中的体积占⽐；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的⽅式助溶)1.请依序添加每种溶剂：10%DMSO>>40%PEG300>>5%Tween-80>>45%salineSolubility:≥1.25mg/mL(3.17mM);Clearsolution1/3MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemE2.请依序添加每种溶剂：10%DMSO>>90%(20%SBE-β-CDinsaline)Solubility:≥1.25mg/mL(3.17mM);Clearsolution3.请依序添加每种溶剂：10%DMSO>>90%cornoilSolubility:≥1.25mg/mL(3.17mM);ClearsolutionBIOLOGICALACTIVITY⽣物活性BC-1215⼀种F-box蛋⽩3(Fbxo3)抑制剂。BC-1215通过拮抗Fbxo3对TRAF细胞因⼦信号传导起作⽤，并在体外表现出低IC50。BC-1215可⽤于炎症研究。体外研究BC-1215(0,0.4,2,10,50µg/mL;16h,18hand24h)inhibitstheFbxo3-TRAFactivationpathwaybydestabilizingTRAF1-6[1].WesternBlotAnalysis[1]CellLine:Murinelungepithellal(MLE)cells,PBMCcellsandU937cellsConcentration:0,0.4,2,10,50µg/mLIncubationTime:6h,18hand24hResult:DecreasedTRAFproteinandinhibitedabroadspectrumofTh1panelcytokines.体内研究BC-1215(i.p.;100μg)reducesbacterial-inducedinflammationa[1].AnimalModel:Cecalligationandpuncture(CLP)-inducedsepsismodel[1]Dosage:100μgAdministration:i.p.Result:SignificantlyattenuatedCLP-inducedsecretionofallthreecirculatingpro-inflammatorycytokinesanddecreasedbacterialcountsintheCLPmodel.户使⽤本产品发表的科研⽂献•IntJMolSci.2022Nov7;23(21):13648.Seemorecustomervalidationsonwww.MedChemEREFERENCES[1].ChenBB,etal.AcombinatorialFboxproteindirectedpathwaycontrolsTRAFadaptorstabilitytoregulateinflammation.NatImmunol.2013May;14(5):470-9.2/3MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemEMcePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresear