BA-53038B-DataSheet-生命科学试剂-MedChemExpress

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemEBA-53038BCat.No.:HY-114314CASNo.:2306195-65-1分⼦式:C₁₄H₁₆ClNO分⼦量:249.74作⽤靶点:HBV作⽤通路:Anti-infection储存⽅式:Powder-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性数据体外实验DMSO:250mg/mL(1001.04mM;Needultrasonic)MassSolvent1mg5mg10mgConcentration制备储备液1mM4.0042mL20.0208mL40.0416mL5mM0.8008mL4.0042mL8.0083mL10mM0.4004mL2.0021mL4.0042mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存⽅式和期限：-80°C,6months;-20°C,1month。-80°C储存时，请在6个⽉内使⽤，-20°C储存时，请在1个⽉内使⽤。BIOLOGICALACTIVITY⽣物活性BA-53038B⼀种HBV核⼼蛋⽩变构调节剂(CpAM)，与HAP⼝袋结合并调节HBV⾐组装。BA-53038B对⼄型肝炎病毒(HBV)具有抗病毒活性，EC50值为3.32μM。BA-53038B可⽤于慢性⼄型肝炎的研究。IC50&TargetEC50:3.32μM(HBV)[1]1/2MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemE体外研究BA-53038BhasantiviralactivityforHBVwithanEC50valueof3.32μM[1].BA-53038BhascytotoxicitywithCC50valueof>100μM[1].BA-53038B(5μM;48h)inducesthecapsid/nucleocapsidmobilityshiftandreducedtheamountofhypophosphorylatedcoreprotein[1].BA-53038B(0-20μM;2or6days)inhibitsHBVnucleocapsidassembly[1].BA-53038B(5μM;2days)inhibitspgRNAencapsidationandconsequentiallyreducestheamountofDNA-containingcapsidsandhypophosphorylatedcoreprotein[1].BA-53038B(2μM;6h)modulatesHBVcapsidassemblybybindingtotheHAPpocket[1].BA-53038BhasresistanteffectwithanEC50valueof>10μMonHBVcapsid(HepG2cellstransientlytransfectedpHBV1.3/core-V124W)[1].WesternBlotAnalysis[1]CellLine:AML12HBV10cellsConcentration:5μMIncubationTime:48hResult:Reducedtheamountofhypophosphorylatedcoreproteinandpromotedtheassemblyofemptycapsidswithslowelectrophoresismobility.RT-PCR[1]CellLine:AML12HBV10andHepDES19cellsConcentration:0-20μMIncubationTime:2or6daysResult:InhibitedHBVreplicationinbothAML12HBV10andHepDES19cells.REFERENCES[1].ZhangX,etal.DiscoveryofNovelHepatitisBVirusNucleocapsidAssemblyInhibitors.ACSInfectDis.2019May10;5(5):759-768.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearchuseonly.