LDC7559-DataSheet-生命科学试剂-MedChemExpress

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemELDC7559Cat.No.:HY-111674CASNo.:2407782-01-6分⼦式:C₂₀H₁₉N₃O₃分⼦量:349.38作⽤靶点:Pyroptosis作⽤通路:Apoptosis;Immunology/Inflammation储存⽅式:Powder-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性数据体外实验DMSO:100mg/mL(286.22mM;Needultrasonic)MassSolvent1mg5mg10mgConcentration制备储备液1mM2.8622mL14.3111mL28.6221mL5mM0.5724mL2.8622mL5.7244mL10mM0.2862mL1.4311mL2.8622mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存⽅式和期限：-80°C,6months;-20°C,1month。-80°C储存时，请在6个⽉内使⽤，-20°C储存时，请在1个⽉内使⽤。体内实验请根据您的实验动物和给药⽅式选择适当的溶解⽅案。以下溶解⽅案都请先按照InVitro⽅式配制澄的储备液，再依次添加助溶剂：(为保证实验结果的可靠性，澄的储备液可以根据储存条件，适当保存；体内实验的⼯作液，建议您现⽤现配，当天使⽤；以下溶剂前显⽰的百分⽐指该溶剂在您配制终溶液中的体积占⽐；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的⽅式助溶)1.请依序添加每种溶剂：10%DMSO>>40%PEG300>>5%Tween-80>>45%salineSolubility:≥2.08mg/mL(5.95mM);Clearsolution1/2MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemE2.请依序添加每种溶剂：10%DMSO>>90%(20%SBE-β-CDinsaline)Solubility:≥2.08mg/mL(5.95mM);Clearsolution3.请依序添加每种溶剂：10%DMSO>>90%cornoilSolubility:≥2.08mg/mL(5.95mM);ClearsolutionBIOLOGICALACTIVITY⽣物活性LDC7559⼀种消⽪素D(GSDMD)的抑制剂，通过抑制中性粒细胞胞外杀⽹络(NET)后期阶段⽽发挥作⽤。IC50&TargetGasderminD[1].体外研究LDC7559(1and5μM)inhibitsIL-1βreleaseuponinflammasomeactivation,andsignificantlyblocksthelethaleffectofbothhumanandmurineGSDMDNTdomainstransfectedintoHEK293Tcells[3].LDC7559functionsdirectlythroughblockingtheactivityoftheGSDMDNTdomain,ratherthaninterferingwiththeactivationandcleavageprocess[3].户使⽤本产品发表的科研⽂献•Elife.2021Jul21;10:e68755.•Elife.2021Jul21;10:e68755.•EurJImmunol.2022Oct17.•FASEBJ.2020Oct;34(10):14024-14041.Seemorecustomervalidationsonwww.MedChemEREFERENCES[1].SpelL,etal.GasderminDopensthewayforNETs.NatRevRheumatol.2018Dec;14(12):690-692.[2].SollbergerG,etal.GasderminDplaysavitalroleinthegenerationofneutrophilextracellulartraps.SciImmunol.2018Aug24;3(26).pii:eaar6689.[3].AnkitPandeya,etal.GasderminD(GSDMD)asaNewTargetfortheTreatmentofInfection.Medchemcomm.2019Apr4;10(5):660-667.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearchus