分子生物学英文版教学课件：Chapter 10 Recombination

Chapter10Recombination/重组10.1HomologousRecombination10.1.1MechanismforCrossing-Over10.1.2MechanismforDouble-StrandedBreakRepair10.1.3TheRecBCDpathway10.1.4GeneConversion10.2Non-homologousRecombination10.2.1Transposons10.2.2Retrotransposons10.2.3Bacteriophage

λintegration10.1同源重组

10.1.1交换机理

10.1.2双链断裂修复机理

10.1.3RecBCD途径

10.1.4基因转换10.2非同源重组

10.2.1转座子

10.2.2反转录转座子

10.2.3λ噬菌体的整合10.1HomologousRecombination同源重组Diploid(2n)2copiesofeachchromosomeHomologousRecombinationIfthesechromosomeswereleftunchanged…ChildGrand-childIdenticalCrossing-overinmeiosisChildGrand-childDifferentHomologousRecombinationinDNArepairHomologouschromosomes10.1.1MechanismforCrossing-Over/交换机理Mechanismofrecombinationinyeast(1/2)Mechanismofrecombinationinyeast(2/2)HollidayjunctionsBranchmigrationofHollidayjunctions

Holliday交叉的分支迁移ResolutionoftheHollidayjunction

Holliday交叉的拆分ResolutionoftheHollidayjunction

tocreateacrossover

产生交换的Holliday交叉拆分ResolutionofHollidayjunctions

fornon-crossover

不产生交换的Holliday交叉拆分ResolutionoftheHollidayjunction

tocreateacrossover

产生交换的Holliday交叉拆分10.1.2MechanismforDouble-StrandedBreakRepair/双链断裂修复机理ResolutionofHollidayjunctions

fornon-crossover

不产生交换的Holliday交叉拆分Brca1/Brca2deletionandbreastcancerBrca1/Brca2缺失与乳腺癌/cancer/breast.htm

TherolesofBRCA1andBRCA2inDNArepairBRCA1和BRCA2在DNA修复中的作用www-ermm.cbcu.cam.ac.uk/01003118h.htm

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Meselson-Radding

HeteroduplexModelFile:biophoto7\IntroductiontoGeneticAnalysis\

Meselson-Radding

heteroduplexModel10.1.3TheRecBCDPathway(1/3)TheRecBCDPathway(2/3)TheRecBCDPathway(3/3)10.1.4GeneConversion/基因转换GTATGCATHomologous,Notidentical.3’5’3’5’3’5’3’5’GeneConversion/基因转换GeneConversion/基因转换Neurospora

crassa

粗糙脉孢菌++mmMeiosisI减数分裂IMeiosisII减数分裂IIMitosis有丝分裂++mm++mmmmmm++++4:4Neurospora

crassa

粗糙脉孢菌/Initiatives/Models/

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4:45:36:2Nowshowing

GeneConversionFile:biophoto7IntroductiontoGeneticAnalysisGeneticConsequencesofMeselson-RaddingModel10.2Non-homologousRecombination

非同源重组10.2.1Transposons

转座子10.2.2Retrotransposons

反转录转座子10.2.3Bacteriophageλintegration

λ噬菌体的整合10.2.1Transposons

/转座子Transposition/转座Generationofdirectrepeats

同向重复序列的产生Nowshowing

ReplicativetranspositionFile:biophoto7\

IntroductiontoGeneticAnalysis\Replicativetransposition.(5’)10.2.2Retrotransposons

反转录转座子LTR-Retrotransposons

LTR反转录转座子Non-LTRRetrotransposons

非LTR反转录转座子LTR-Retrotransposons

LTR反转录转座子Non-LTRretrotransposons

非LTR反转录转座子Weaver’sMolecularBiology,3rdEdition,Figure23.3110.2.3