在食物诊断的趋向和需要

TrendsandNeedsinFoodDiagnosticsLarissaKahr-BIT,AustriaMalinBrännback-TEKES,FinlandGabrielavonBlankenfeld-Enkvist-TEKES,FinlandAimoftheStudyInvestigatetechnologicaltrendsandneedsinFoodDiagnosticsUnderstandthefactorsthatshapeandinfluencethedevelopmentofthisfield.BureauforInternationalResearchandTechnologyCooperation-AustriaFocusBackgroundonfoodsafetymanagementtoolsDescriptionofthemethodscurrentlyusedandnewmethodsdevelopedThemarketforfooddiagnostics

BureauforInternationalResearchandTechnologyCooperation-AustriaDefinitionsFoodDiagnosticsappliesmodernmethodsdevelopedinhumaninvitrodiagnostictofoodRapidMethodscomprisesdifferenttypesoftestsMiniaturisedbiochemicalkitsAntibody-basedtestsNucleicacidhybridisation-basedtestsManual,semi-automatedorfullyautomated

BureauforInternationalResearchandTechnologyCooperation-AustriaDetectionBacteria,viruses,parasites,chemicals,biotoxins,heavymetalsandprionsInallstepsofthefoodchain-fromrawmaterialstoendproducts

BureauforInternationalResearchandTechnologyCooperation-AustriaFoodastestmatrixExtremelyvariedinitschemicalcompositionContainsmanydifferentingredientsMightcontainanintrinsicmicrobialfloraBureauforInternationalResearchandTechnologyCooperation-AustriaFrameFoodSafetyProcesscontrolismoreefficientthanproductcontrolThesafetyofaproductwithlowdefectratescannotbeguaranteedthroughsamplingandtestingThefocusischangingfromend-producttestingtoHACCPThishastobeconsideredwhendevelopingnewmethodswithintheconceptualframeoffoodsafetyBureauforInternationalResearchandTechnologyCooperation-AustriaMicrobiologicalmethodscanbedividedinto“traditional”or“conventional”methodsandrapidmethods

BureauforInternationalResearchandTechnologyCooperation-AustriaConventionalMethodsConventionalmethodsrelyonculturingmicroorgansimsontoagarplatesLaboriousandtime-consuming84%ofalltestsarebasedoncountingviablecellsRequiredbynationalandinternationalregulatoryagenciesforofficialcontrolBureauforInternationalResearchandTechnologyCooperation-AustriaRapidMethodsAdvantagesinanalysistimePossibilitytoeliminatelabourintensivestepsPotentialforautomationHowever,lengthyincubationproceduresarestillnecessaryFirstone-shiftmethodsunderdevelopmentELISAandPCRaredominatingLackofacommonvalidationprocedureMostlynotacceptedforofficialcontrolpurposesBureauforInternationalResearchandTechnologyCooperation-AustriaMethodsAdvancesinViableCellCountMethodsMiniaturisationandDiagnosticKits,BiochemicalIdentificationTechniquesAntibody-basedmethodsImmunomagneticSeparationNucleicacid-basedassaysBiosensorsMicroarraysFlowCytometryBacteriophage-BasedTechniquesATPbioluminescenceAdenylatekinasebioluminescenceRiboprintingandPulse-FieldGelElectrophoresisBureauforInternationalResearchandTechnologyCooperation-AustriaViablecellcountingWillremainimportantfortheassessmentofsafetyandqualityoffoodproductsdevelopmentofsemi-automatedalternativemethodsnewmethodsarebasedongrowthandmetabolicactivityMicroscopicmethodsflowcytometryandcellsorting(FACS)BureauforInternationalResearchandTechnologyCooperation-AustriaImmunoassaysELISA(withdipsticktechnology),ImmunochromatographyFullyautomatedsystemsalreadyonthemarketNewrecombinantantibodiesandmolecularimprintingtechniquestoimprovesensitivityandversatilityBureauforInternationalResearchandTechnologyCooperation-AustriaImmunomagneticSeparationSpeedupenrichmentstepsNodetectionmethoditselfCombinationwithdifferentend-detectionmethodstoimprove-Speedandsensitivity-Samplethroughput-Userfriendliness-AutomationexpectedinthenextfutureBureauforInternationalResearchandTechnologyCooperation-AustriaNucleicacidbasedassayDirectlyorafteramplificationwithPCRDevelopmentofhomogenousassaysandautomationimprovesroutineuseandreducesfalsepositiveresultsafterPCRNewassayformatsunderdevelopmentBureauforInternationalResearchandTechnologyCooperation-AustriaBiosensorsandMicroarraysOn-lineoron-site,sensitive,low-cost,rapidmethodsforroutine-usePrototypesunderdevelopmentHavehighpotentialforautomationSimpleandportableequipmentcanbedesignedApplicationswithinqualityandprocesscontrol,controloffermentationprocesses,qualityandsafetycontrolofrawmaterialsandforHACCPmonitoringBureauforInternationalResearchandTechnologyCooperation-AustriaHygienemonitoringNeedtodeveloprealtimemethodstomonitorcleaningprocedureMethodsshouldbecheap,robustATPbioluminescence,Adenylatekinase,biosensorapplicationsmightbefurtherdevelopedBureauforInternationalResearchandTechnologyCooperation-AustriaMethodsImplementationInhousealternativemethodsforviablecellcountshygienemonitoringchemicalanalysisOutsourcingpathogentestingBureauforInternationalResearchandTechnologyCooperation-AustriaTheMarketforFoodDiagnosticsDefinitionsFoodAnalysis:allfoodtestingFoodDiagnostics:foodtestingwithrapidmethodsMicrobiologicalTesting:alltestingformicroorganismsFoodMicrobiologicalTesting:alltestingformicroorganismsinfoodandbeveragesBureauforInternationalResearchandTechnologyCooperation-AustriaTheMarketforFoodDiagnostics

SomefiguresFoodanalysisglobalmarket€1.1billionSharedbyabout50companiesUSAandEuropemakeupabout2/3€565millionglobalsalesforFoodmicrobiologicaltestsRapidmethods€115millionora20%totalmarketshareBureauTechnologySales($USmillions)MarketShare(%)ELISA/EIA7061DNA2522Impedance109FlowCytometry54ATP54Total115100BureauforInternationalResearchandTechnologyCooperation-Austria

FoodAnalysis(€million)

FoodDiagnostics(€million)%FoodDiagnostics(€million)Foodpathogens97,51315%Spoilage22037,517%Chemicalcontaminants5319,540%Processchemicals1201610%Total490,586

BureauforInternationalResearchandTechnologyCooperation-AustriaValidationAOACvalidationbodyintheU.S.NordValandMicroValaretheNordicandEuropeanbodiesProblem:lackofacommonvalidationprocedureInhibitsestablishmentofrapidmethodsBureauforInternationalResearchandTechnologyCooperation-AustriaConclusionsManynewmethodsandtechnologiesarebeingdevelopedIncreasedsamplethroughputandefficiencythroughautomationThemarketforrapidmethodsissmallPenetrationofrapidmethodshasbeenslowLackofacommonvalidationprocedureinhibitsacceptanceofnewmethodsTheincentivetotransfertechnologiesmightbetoolowBureauforInternationalResearchandTechnologyCooperation-AustriaRecommendationsMicroarraysandbiosensorsshouldbetechnologicallyevaluatedNewmarketopportunitieswithinqualityandprocesscontrolshouldbeexploredThedevelopmentofnewmethodsshouldalwaysincludeeffortstoimplementthenewmethodTheformationofaprivateservicesectorshouldbeencouragedBureauforInternationalResearchandTechnologyCooperation-AustriaBureauforInternationalResearchandTechnologyCooperation-AustriaSecondaryFoodProcessingDomesticFoodPreparationFoodretailing

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