UZH1a-DataSheet-生命科学试剂-MedChemExpress

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemEUZH1aCat.No.:HY-134673A分⼦式:C₃₂H₄₂N₆O₃分⼦量:558.71作⽤靶点:Apoptosis作⽤通路:Apoptosis储存⽅式:Powder-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性数据体外实验DMSO:80mg/mL(143.19mM;Needultrasonic)MassSolvent1mg5mg10mgConcentration制备储备液1mM1.7898mL8.9492mL17.8984mL5mM0.3580mL1.7898mL3.5797mL10mM0.1790mL0.8949mL1.7898mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存⽅式和期限：-80°C,6months;-20°C,1month。-80°C储存时，请在6个⽉内使⽤，-20°C储存时，请在1个⽉内使⽤。体内实验请根据您的实验动物和给药⽅式选择适当的溶解⽅案。以下溶解⽅案都请先按照InVitro⽅式配制澄的储备液，再依次添加助溶剂：(为保证实验结果的可靠性，澄的储备液可以根据储存条件，适当保存；体内实验的⼯作液，建议您现⽤现配，当天使⽤；以下溶剂前显⽰的百分⽐指该溶剂在您配制终溶液中的体积占⽐；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的⽅式助溶)1.请依序添加每种溶剂：10%DMSO>>40%PEG300>>5%Tween-80>>45%salineSolubility:≥5.5mg/mL(9.84mM);Clearsolution2.请依序添加每种溶剂：10%DMSO>>90%(20%SBE-β-CDinsaline)Solubility:≥5.5mg/mL(9.84mM);Clearsolution1/2MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemE3.请依序添加每种溶剂：10%DMSO>>90%cornoilSolubility:≥6mg/mL(10.74mM);ClearsolutionBIOLOGICALACTIVITY⽣物活性UZH1a⼀种有效和选择性的METTL3抑制剂，IC50值为280nM。UZH1a可⽤于细胞进程的转录组调控。UZH1a具有抗肿瘤活性。UZH1a也可⽤作研究METTL3的化学探针。IC50&TargetIC50:280nM(METTL3)[1]体外研究UZH1a(2.5-160µM;72h)inhibitsthegrowthofMOLM-13,HEK293T,andU2Oscells,withIC50sof11µM,67µM,and87µM,respectively[1].UZH1a(2.5-100µM;16h)reducesm6AmethylationlevelinmRNAfromMOLM-13cellsinadose-dependentmanner(IC50=4.6µM)[1].UZH1a(40µM;16h)reducesm6AmethylationlevelinmRNAfromMOLM-13,HEK293T,andU2Oscells[1].UZH1a(20µM;16h)increasesapoptosisandleadstocellcyclearrestinMOLM-13cells[1].CellViabilityAssay[1]CellLine:MOLM-13,HEK293T,andU2OscellsConcentration:2.5-160µMIncubationTime:72hoursResult:InhibitedthegrowthofMOLM-13,HEK293T,andU2Oscellsinadose-dependentmanner.REFERENCES[1].Moroz-OmoriEV,et,al.METTL3inhibitorsforepitranscriptomicmodulationofcellularprocesses.bioRxiv.2020Oct13.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearchuseonly.T