Protein-deglycase-DJ-1-against-1-DataSheet-生命科学试剂-MedChemExpress

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemEProteindeglycaseDJ-1against-1Cat.No.:HY-137262CASNo.:724737-74-0分⼦式:C₂₄H₂₃N₃O₄分⼦量:417.46作⽤靶点:Others作⽤通路:Others储存⽅式:Powder-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性数据体外实验DMSO:25mg/mL(59.89mM;ultrasonicandadjustpHto7withNaOH)MassSolvent1mg5mg10mgConcentration制备储备液1mM2.3954mL11.9772mL23.9544mL5mM0.4791mL2.3954mL4.7909mL10mM0.2395mL1.1977mL2.3954mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存⽅式和期限：-80°C,6months;-20°C,1month。-80°C储存时，请在6个⽉内使⽤，-20°C储存时，请在1个⽉内使⽤。体内实验请根据您的实验动物和给药⽅式选择适当的溶解⽅案。以下溶解⽅案都请先按照InVitro⽅式配制澄的储备液，再依次添加助溶剂：(为保证实验结果的可靠性，澄的储备液可以根据储存条件，适当保存；体内实验的⼯作液，建议您现⽤现配，当天使⽤；以下溶剂前显⽰的百分⽐指该溶剂在您配制终溶液中的体积占⽐；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的⽅式助溶)1.请依序添加每种溶剂：10%DMSO>>40%PEG300>>5%Tween-80>>45%salineSolubility:≥2.08mg/mL(4.98mM);Clearsolution1/2MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemE2.请依序添加每种溶剂：10%DMSO>>90%cornoilSolubility:≥2.08mg/mL(4.98mM);ClearsolutionBIOLOGICALACTIVITY⽣物活性ProteindeglycaseDJ-1against-1DJ-1结合化合物，依赖地靶向DJ1。ProteindeglycaseDJ-1against-1可以穿透⾎脑屏障(BBB)。ProteindeglycaseDJ-1against-1神经保护剂，具有⽤于帕⾦森病研究的潜⼒。体内研究ProteindeglycaseDJ-1against-1(compound-23;1mg/kg;IP;pretreatmentonehour;daily;for4days)significantlyrestoresMPTPinducedlocomotion,dopaminergicneuronalcelldeathanddopaminecontentinDJ-1(+/+)micebutnotinDJ-1(-/-)mice[1].AnimalModel:Wild-typemaleC57BL/6miceat8,11-12and9-12weeks;DJ-1-knockoutmalemiceat11-13weeks[1]Dosage:1mg/kgAdministration:IP;pretreatmentonehour;daily;for4daysResult:SignificantlyrestoredMPTP(30mg/kg;IP;Onehourafterinjection)inducedlocomotion,dopaminergicneuronalcelldeathanddopaminecontentinDJ-1(+/+)micebutnotinDJ-1(-/-)mice.REFERENCES[1].KazukoTakahashi-Niki,etal.DJ-1-dependentprotectiveactivityofDJ-1-bindingcompoundno.23againstneuronalcelldeathinMPTP-treatedmousemodelofParkinson'sdisease.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearchuseonly.T