Laminaran-DataSheet-生命科学试剂-MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemELaminaranCat. No.: HY-119109CAS No.: 9008-22-4作靶点: Others作通路: Others储存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性数据体外实验 DMSO : 100 mg/mL (Need ultrasonic)请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液，并请注意储备液的保存式和期限。体内

2、实验 请根据您的实验动物和给药式选择适当的溶解案，配制前请先配制澄的储备液，再依次添加助溶剂(为保证实验结果的可靠性，体内实验的作液，建议您现现配，当天使；澄的储备液可以根据储存条件，适当保存；以下溶剂前的百分指该溶剂在您配制终溶液中的体积占)：1. 请依序添加每种溶剂： 10% DMSO 90% (20% SBE-CD in saline)Solubility: 2.5 mg/mL (Infinity mM); Clear solution2. 请依序添加每种溶剂： 10% DMSO 90% corn oilSolubility: 2.5 mg/mL (Infinity mM); Clear

3、 solutionBIOLOGICAL ACTIVITY物活性 Laminaran种中性溶性 -1-3-葡聚糖，从褐藻双歧杆中分离，Dectin-1 的典型配体，具有强的免疫调节，辐射防护作 ，以及抗癌活性。 Laminaran 由具有 (16) 分的 (13)-葡聚糖组成，可以被葡糖酶诸如 (EC ) 之类的酶催化，该酶会破坏 (13) 键。 Laminaran种有前途的免疫刺激分，可于癌症免疫治疗。体外研究Laminaran (100-800 g/mL; 24 hours) is not cytotoxic against normal epidermal cells JB6 Cl41 a

4、nd humanmelanoma cells SK-MEL-28, the percentage of inhibition of living cells number is less than 15 % atconcentrations range up to 800 g/mL after 24 h of treatment 1.Laminaran (200 g/mL; 24-72 hours)doesnot cause any growth inhibition of SK-MEL-28 cells after 24 and 48 h of treatment, but decrease

5、s cellsproliferation by 36 % after 72 h of treatment 1.Laminaran (25-50 g/mL; 24 hours) at low concentration does1/3 Master of Small Molecules 您边的抑制剂师www.MedChemEnot influence the phosphorylation of c-Raf (Ser259), ERK1/2 (Tyr202/Tyr204), and MEK1/2 (Ser 221) kinasesas well as total expression level

6、 of investigated proteins. But decreases p-MEK, p-ERK1/2 at 50 g/mL 1.Cell Viability Assay 1Cell Line: JB6 Cl41 and SK-MEL-28 cellsConcentration: 100, 200, 400, and 800 g/mLIncubation Time: 24 hoursResult: Showed no cytotoxic against normal epidermal cells JB6 Cl41 and human melanomacells SK-MEL-28.

7、Cell Proliferation Assay 1Cell Line: SK-MEL-28 cellsConcentration: 200 g/mLIncubation Time: 24 hoursResult: Decreased cell proliferation at 72 hours.Western Blot Analysis 1Cell Line: SK-MEL-28 cellsConcentration: 25 g/mL; 50 g/mLIncubation Time: 24 hoursResult: Inhibited phosphorylation of c-Raf, ME

8、K1/2, and ERK1/2 kinases.体内研究 Laminaran (intravenous injection; 12.5, 25, and 50 mg/kg; 21 days) and OVA (50 g) combination significanlydecreases the tumor masses when it compares with the PBS-, OVA-, and laminarin-treated mice 3.Animal Model: C57BL/6 mice injected s.c. with B16-OVA cells 3Dosage: 1

9、2.5, 25, and 50 mg/kg; 21 daysAdministration: Intravenous injectionResult: Prevented B16-OVA tumor growth by inducing Ag-specific immune responses.REFERENCES1. Malyarenko OS, et al. Laminaran from brown alga Dictyota dichotoma and its sulfated derivative as radioprotectors and radiosensitizers2/3 Ma

10、ster of Small Molecules 您边的抑制剂师www.MedChemEin melanoma therapy.Carbohydr Polym. 2019 Feb 15;206:539-547.2. Laminaran3. Song K, et al. Laminarin promotes anti-cancer immunity by the maturation of dendritic cells.Oncotarget. 2017 Jun 13;8(24):38554-38567.McePdfHeightCaution: Product has not been fully valida