荧光探针的发展和应用

1、Supplementary materials for:Perylene diimide based “turn-on” fluorescence sensor for detection of Pd2+ in mixed aqueous mediaHai-xia Wang a, b,*, Yue-he Lang a, Hui-xuan Wang a, Jing-jing Lou a, Hai-ming Guo a, b, Xi-you Li c,*a School of Chemistry and Chemical Engineering, Key Laboratory of Green C

2、hemical Media and Reactions of Ministry of Education, Henan Normal University, Xinxiang 453007, China b School of Environmental Science, Henan Normal University, Xinxiang 453007, China email: HYPERLINK mailto: (H. Wang)c School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100

3、, China email: HYPERLINK mailto:xiyouli xiyouli (X. Li)ContentsPage S1: Title of the paper, authors along with the contents.Page S2-S4: Copy of the 1H and 13C NMR spectra of PDI-1, PDI-2 and PDI-3.Page S5: Photophysical properties of PDI-1, PDI-2 and PDI-3 derivatives in different solvents at room t

4、emperature; Fluorescence spectra change of PDI-2 and PDI-3 upon addition of different metal(8.0 equiv) ions; UV-vis spectra of PDI-1 (6.0 M) in the presence of different metal ions (8.0 equiv).Page S6: Job Js plots in DMF/H 2O (v/v, 7/1) and acetonitrile; Fluorescence spectra changes of PDI-1 (5.0 M

5、) in the presence of Pd2+in acetonitrile and chloroform; ESI mass spectra of PDI-1 in the presence of 1.0 equiv PdCl2 in CH3CN.Page S7: Jobs plots of PDI-1 (5.0 jM) in the presence of Pd2+in chloroform; Influence of pH on fluorescence intensity of PDI-1 (5.0 M) in the absence and presence of 1.0 eq

6、Pd2+; Benesi-Hildebrand analysis results.粽E局 Ln昌昌 君三缶 is 6NE韩 导g着m EME_ ZLWL 着EL E挡身L 国奇L 希DH 富邑 导一单 WSL 雀.蜀 ELnD.胃 L 泛骂理 S.E 岑爵宜 邑2 号X.EM 京帛L _sg孚 管.培L- 中5骂 命皇 8胃E孚 2E段LHWMi.i iw.miiim ，rFig. S2 13C NMR (100MHz) spectrum of PDI-1 in CDC13.Fig. S3 1H NMR (400 MHz) spectrum of PDI-2 in CDCl3.+心IN 6gl

7、制也E F5 心EIEwkggr-0v、00B黑胃禹.？1却闵日旧局学挡E-吁导6 导一TE 翊苗 xS岑备 空3JaCS.M .1DE富15Q1Cd50DppmFig. S4 13C NMR (100 MHz) spectrum of PDI-2 in CDC.IDoFig. S5 1H NMR (400 MHz) spectrum of PDI-3 in CDC13LiE局,s号h-LlnW9T本ELL 密羿！r 昌孕 SWELL so.扃 L 切寻CEL 3-g.蜀 L 莅RL D 贸 ZZL Q馈忍L h-QLBs&EIr m欧L s育z. 房与L 5籍蠲L L舄斜L -X-将.SSL

8、 昌*L100ISO100叩mFig. S6 13C NMR (100 MHz) spectrum of PDI-3 in CDCl3.Table S1: Photophysical properties of PDI-1, PDI-2 and PDI-3 derivatives in different solvents at room temperaturePDT-1PDT-2PDT-3Toluene5734.726050.0062572DCE5774.706130.021577Toluene5734.726050.0062572DCE5774.706130.021577DCM5784.7

9、06140.010578THF5684.696050.0043569Dioxane5674.735960.0052568CH*N5694.716040.0063569DMSO5774.696000.0030578入absmaxnm脸 8k emmaxM-1cm-1nmFbgekem中kabskgkemFmaxFmaxmaxM-1cm-1nmnmM-1cm-1nm4.586030.0805724.646040.494.536080.0835774.636110.444.526140.0905784.636130.504.655980.0825674.636000.364.665980.09156

10、74.656010.434.646040.0795684.096010.394.576110.0415774.406140.24kabsmaxnmWMlengih inmiFig. S7 Fluorescence spectra change of PDI-2 (left) and PDI-3 (right) upon addition of different metal (8.0 equiv) ions including Cr3+, Mn2+, Fe3+, Co2+, Ni2+, Cu2+, Zn2+, Ag+, Cd2+, Hg2+, Pb2+ and Pd2+ in DMF/H2O

11、(v/v, 7:1),入ex =540 nm, Slit: 2.5 nm; 5.0 nm.0 306007005 0J 2 1 1 Q ” ” o 心oueq0 306007005 0J 2 1 1 Q ” ” o 心oueq|Pd +PDl-11Fig. S12 (left) fluorescence spectra of PDI-1 (5.0 gM) in the presence of different concentrations of Pd2+ in chloroform after 30 minutes,入ex = 540 nm, Slit: 2.5 nm; 5.0 nm; (r

12、ight) Jobs plot of PDI-1 in chloroform showing the 1:1 stoichiometry of the complex between Pd2+ and PDI-1, the total of the chemosensor and Pd2+ is 10 gM,入ex = 540 nm, Slit: 2.5 nm; 5.0 nm.一一PD一一PD卜1 PDlM+IPd2*900800700600500-00-300200 10001235678910PHFig. S13 Influence of pH on fluorescence intens

13、ity of PDI-1 (5.0 gM) in the absence and presence of 1.0 eq Pd2+, 入ex = 540 nm, Slit: 5.0 nm; 5.0 nm. The pH of solution was adjusted by aqueous solution of NaOH (1.0 M) or HCl (1.0 M). Inset showing a influence of pH on fluorescence for PDI-1.Benesi-Hildebrand equation and Benesi-Hildebrand analysis results are as follows:111=+F - F F