无机盐缓冲液配方表

1、250.1mol/l 磷酸钠缓冲液的配制pH1mol/lNa2HPO4(ml)1mol/lNaH2PO4(ml)5.87.992.16.012.088.06.217.882.26.425.574.56.635.264.86.846.353.77.057.742.37.268.431.67.477.422.67.684.515.57.889.610.48.093.26.80.2mol/L磷酸盐缓冲液pH5.78.0pH0.2mol/L NaH2PO4(ml)0.2mol/L Na2HPO4(ml)5.793.56.55.892.08.05.990.010.06.087.712.36.185.01

2、5.06.281.518.56.377.522.56.473.526.56.568.531.56.662.537.56.756.543.56.851.049.06.945.055.07.039.061.07.133.067.07.228.072.07.323.067.07.419.081.07.516.084.07.613.087.07.710.589.57.88.591.57.97.093.08.05.394.7NaH2PO42H2O：10.86105g或 NaHPO412H2O：21.8502g + NaH2PO42H2O：6.08595g或 NaH2PO4H2O：10.76g1L上述药品

3、都是极易溶的，直接溶解就行了-磷酸二氢钠缓冲液0.2mol/LpH值；0.2mol/LNa2HPO4溶液mL；0.2mol/LNaH2PO4溶液mL5.8 ；8.0；92.05.9 ；6.0；6.1 ；6.2 ；6.3 ；6.4 ；6.5 ；6.6 ；6.7 ；10.0 ；12.3 ；15.0 ；18.5 ；22.5；26.5 ；31.5 ；37.5；43.5 ；90.087.785.081.577.573.568.562.556.56.8 ；49.0；51.06.9；7.0 ；7.1 ；7.2 ；7.3 ；55.0 ；61.0 ；67.0 ；72.0 ；77.0 ；45.039.033.02

4、8.023.07.4；81.0；19.07.5；84.0 ；16.07.6；87.0；13.07.7；89.5；10.57.8；91.5；8.57.9；93.5 ；7.58.0；94.7；5.3NaH2PO42H2O：Mr=178.05，0.2mol/L 溶液为 35.61g/L;NaHPO412H2O：Mr=358.22，0.2mol/L 溶液为 71.64g/L;NaH2PO42H2O：Mr=156.03，0.2mol/L 溶液为 31.21g/L;NaH2PO4H2O：Mr=138.01，0.2mol/L 27.6g/L。磷酸缓冲液phosphate buffer依据下表所给定的体积，混

5、合 1 mol/L 的磷酸二氢钠单碱1mol/L 磷酸氢二钠双碱贮液，获得所需pH 的磷酸缓冲液。1 mol/L 的磷酸二氢钠NaH2PO4“H2O138g 1L;1mol/L 磷酸氢二钠Na2HPO4贮液:142g 1L。1mol/L磷酸二氢钠ml1mol/L磷酸氢二钠mlpH 值8771236.08501506.18151856.27752256.37352656.46853156.56253756.65654356.75104906.84505506.93906107.03306707.12807207.21mol/L磷酸二氢钠ml1mol/L磷酸氢二钠mlpH 值8771236.085

6、01506.18151856.27752256.37352656.46853156.56253756.65654356.75104906.84505506.93906107.03306707.12807207.2ph0.2mol/lNaH2PO4(ml)0.2mol/lNa2HPO4(ml)5.793.56.55.892.08.05.990.010.06.087.712.36.185.015.06.281.518.56.377.522.56.473.526.56.568.531.56.662.537.56.756.543.56.851.049.06.945.055.07.039.061.07.133.067.07