“细胞生物学”教学大纲

1、 10“细胞生物学”教学大纲四川大学生命科学学院AGeneralOutlineoftheCellBiologyCollege of Life Sciences, Sichuan University一、课程根本信息课程名称中、英文：细胞生物学 Cell Biology课程类别：专业根底课 版社，2022.6Cell and Molecular Biology: Concepts and Experiments, 6th n Karp, 2022Molecular Biology of the Cell, 5thEdition, Alberts et al., 2022学时：48学分：3课程地位

2、：国家精品课程主讲教师：邹方东、王喜忠、李娟、李沁桐、张咏梅、杨军 等二、教学目的及要求生命科学综合素养教育及试验技能综合培育等方面都具有重要作用。练，同时使学生学会学习，具有自我开拓可获得学问和利用信息的力量。TeachingObjectives:Cell biology is one of the most important courses of current biology, and hasbeen listed as a key curriculum of sciences by the Ministry of Education of Chinaand excellent cur

3、riculum of Sichuan University and Sichuan province . As the basic unit of structure and function for all organisms, cell is both the start and convergepoints of life science.The teaching target of cell biology is to acquaint the students with the basic knowledge,conceptsandfundamentaltheories,aswell

4、asthebriefhistoryandmostadvanced domain of the subject being taught. Furthermore, the students are to come under trainings on their thinking to master the skills of learning, so as to be capable ofexploiting their talents on getting knowledge available and utilizing information.教学根本特色：课堂教学效果显著，课程在国内

5、具有很好影响力和辐射作用；生命科学进展前沿和热点领域；按着学科内容体系、学科内在联系和主从关系，依次确定学问块学问单元的关系，处理好传授学问和力量培育的关系。21Basic teaching characteristicsCell biology is one of the countrys first-class name brand courses, which is performed bilingually and employing the multi-media. The teaching effect in class is rather remarkable and the c

6、onstruction of the course is on the top internal.Frequent updating. Not only an embodiment of basic knowledge, but also areflection of preceding fields and hotspots of life sciences.Taking the police of “education should face the modernization, face the world andface the future” as the guideline. Ac

7、cording to the content system, internalconnections as well as the principal and subordinate relationships of the subject, we established the route: learning block unit point. And this has beenproved exercisable, which follows the teaching rules and scientific developmentallaws and disposes well of t

8、he relations between traditional and modern contents, and the relation between teaching knowledge and training ability as well.Leave some room for innovation, which in favor of reforms on textbook series for21stcentury and teaching substances.三、教学内容Learning Block 1 Review of Cell Biology and Its Res

9、earchingMethods学问单元一 第一章 绪论(2学时)第一节 细胞生物学争辩的内容与现状学问点二、了解细胞生物学的主要争辩内容学问点一、了解细胞的觉察学问点三、了解细胞学的经典时期学问点五、了解细胞生物学学科的形成与进展学问点六、了解细胞生物学的主要学术组织、学术刊物与教科书Unit 1Chapter 1 IntroductionSection 1Contents and Actuality of Researches on Cell BiologyPoint 1. Know that cell biology is a fundamental subject in modern l

10、ife science Point 2 Know the important researching contents on cell biologySection 2A Brief History of Cytology and Cell BiologyPoint 1 Know the discovery of cellPoint 2 Know the establishment of cytology and its significancePoint 3 Know the classical period of cytologyPoint 4 Know the embranchment

11、and development of experimental cytology and cytologyPoint 5 Know the subjects establishment and development of cell biologyPoint 6 Know the main academic organizations, issues and textbooks学问单元二 其次章 细胞的统一性与多样性(2学时)第一节 细胞的根本特征学问点二、把握细胞的根本共性其次节 原核细胞与古核细胞学问点一、了解最小最简洁的细胞支原体学问点三、了解古核细胞古细菌学问点二、理解细胞的大小及其影

12、响因素学问点一、了解病毒的根本学问学问点二、了解病毒在细胞内的增殖学问点三、理解病毒与细胞在起源与进化中的关系Unit2Chapter 2 Diversity and Commonality of CellsSection1 Basic Concepts of CellsPoint 1 Master and apprehend that cells are the basic units of lifePoint 2 Master the commonness of cellSection2Prokaryotic and Eukaryotic CellPoint 1 Know the mini

13、mal and simplest cellmycoplastPoint 2 Know two representation of prokaryotic cell bacteria and cyanobacteriaPoint 3 Know archaeobacteriaSection3EukaryoticCellPoint 1 Apprehend and master the basic structural system of eukaryotic cellPoint 2 Apprehend the size of cells and its regulatorsPoint 3 Appre

14、hend and master the comparison of prokaryotic and eukaryotic cellsPoint 4 Apprehend and master the comparison between plant cell and animal cellSection 4 Noncellular living OrganismVirusPoint 1 Know the basic knowledge of virusPoint 2 Know that virus proliferate (replicate) in cellsPoint 3 Apprehend

15、 the relation between virus and cell in the origin and evolution of life学问单元三 第三章 细胞生物学争辩方法(3学时)第一节 细胞形态构造的观看方法学问点三、了解扫描隧道显微镜学问点二、了解细胞成分的化学显示方法第三节 细胞培育与细胞工程学问点一、理解并把握荧光漂白恢复技术学问点三、理解酵母双杂交技术学问点五、理解放射自显影技术学问点二、了解突变体制备技术学问点三、了解蛋白质组学技术Unit3Chapter 3 Methodology of Cell BiologySection 1 Methods on Cell St

16、ructure ObservationPoint 1 Apprehend and master of light microscopy Point 2 Apprehend and master of electron microscopePoint 3 Know scanning tunneling microscopeSection 2 Analytical Methods of Cell ComponentsPoint 1 Apprehend using super-centrifugation to separate cellular componentsPoint 2 Know the

17、 marking methods of components in cell.Point 3 Apprehend and master localization and qualitative analyses using specialprotein antigenPoint 4 Know the localization and qualitative analyses of special intracellular nucleicacidsPoint 5 Know the quantitative analyses of cytochemistry, apprehend and mas

18、ter flowcytometrySection 3 Cell Culture and Cell EngineeringPoint 1 Master types and methods of cell incubation Point2MastermainachievementsofcellengineeringSection 4 Dynamics of Cell and BiomacromoleculesPoint 1 Apprehend and master fluorescence photobleaching recoveryPoint 2 know the Single Molecu

19、le DetectionPoint 3 Apprehend yeast two-hybrid systemPoint 4 Apprehend fluorescence resonance energy transferPoint 5 Apprehend the technique to trace intracellular dynamic synthesis ofmacromolecules using radioautographySection 5 Model Organisms and Functional Genome ResearchPoint 1 Know model organ

20、isms used in cell biologyPoint 2 Know how to prepare mutantsPoint 3 Know proteome methods学问块二 生物膜系统Learning Block 2Biomembrane System学问单元一 第四章 细胞质膜 (2学时)学问点二、把握膜脂的种类及运动方式 学问点一、理解并把握膜的流淌性 学问点四、了解细胞膜的功能Unit 1Chapter 4 Cell MembraneSection 1 Structure Model of Cell Membrane and constituentsPoint 1 Mast

21、er and apprehend membrane structurePoint 2 Master types of membrane lipids and motilities Point3MastertypesofmembraneproteinsanddetergentsSection2BasicPropertiesandFunctionsofBiomembranePoint 1 Apprehend and master fluidity of membranePoint 2 Apprehend and master the asymmetry of membranePoint 3 Kno

22、w membrane associated cytoskeleton and specialized structures on cell surfacePoint 4 Know functions of membrane学问单元二 第五章 物质的跨膜运输 (2学时)学问点一、把握载体蛋白和通道蛋白转运特点 其次节 ATP泵、Ca2+泵工作原理与功能V F 型质子泵的功能学问点三、把握 ABC 超家族的功能第三节 胞吞作用与胞吐作用学问点一、理解并把握吞噬作用过程与功能学问点三、了解其他类型的胞饮作用学问点四、理解并把握胞吞作用对信号转导的下调或激活Unit2Chapter 5 The Mov

23、ement of Substances Across Cell Membrane Section 1 Membrane Transport Proteins and Transport Across CellMembrane of small moleculesPoint 1 Master the characters of carrier proteins and channel proteinsPoint 2 Master the types and features of small molecules across cell membraneSection 2 ATP-driven P

24、umps and Active TransportPoint 1 Apprehend and master the mechanisms and functions of the Na +-K+ pump andCa2+ pumpPoint 2 Master the functions fo V-type pump and F-type pumpPoint 3 Master the functions of ABC superfamilyPoint 4 Know the relations between ions transport and membrane potentialSection

25、3EndocytosisandExocytosisPoint 1 Apprehend and master the process and functions of phagocytosisPoint2 Apprehend andmaster theprocess and features ofclathrin dependentendocytosisPoint 3 Know other types of pinocytosisPoint 4 Apprehend and master the regulations of endocytosis to signaling学问单元三 第六章 线粒

26、体和叶绿体(2学时)第一节 线粒体与氧化磷酸化学问点一、理解并把握线粒体的形态与动态变化学问点三、理解并把握线粒体能量转换学问点四、了解线粒体与疾病自学学问点二、了解叶绿体的构造和化学组成第三节 线粒体和叶绿体的半自主性及其起源学问点一、了解线粒体和叶绿体的 DNA学问点二、理解线粒体和叶绿体的蛋白质合成学问点四、了解线粒体和叶绿体的增殖与起源Unit3Chapter 6 Mitochondria and ChloroplastSection1MitochondrionandOxidativePhosphorylationPoint 1 Apprehend and master the mor

27、phology and dynamics of mitochondrionPoint 2 Know the chemical composition and localization of enzymes in mitochondrionPoint 3 Apprehend and master functions of mitochondrion for energy conversion Point 4 Know mitochondrion and diseasesSection2ChloroplastandPhotosynthesis(Self-study)Point 1 Know the

28、 modalities, sizes and number of chloroplast Point2KnowstructureandchemicalcompositionofchloroplastPoint3ApprehendandmastermainfunctionofchloroplastphotosynthesisSection 3 Mitochondrion and Chloroplast are Semiautonomous Organelles ，and OriginPoint 1 Know mitochondrion and chloroplast DNAPoint 2 App

29、rehend the protein synthesis of mitochondrion and chloroplastPoint 3 Apprehend the relation between mt/chDNA and nuclear genome Point 4 Know proliferation and origin of mitochondrion and chloroplast学问单元四 第七章 细胞质基质与内膜系统(3学时)学问点一、理解把握内质网蛋白质合成、修饰加工、应激反响学问点三、理解并把握溶酶体类型、功能、发生以及疾病学问点四、了解过氧化物酶体与溶酶体的区分、功能与发

30、生5 第八章 蛋白质分选与膜泡运输(3 学时)学问点二、理解并把握蛋白质分选途径与类型其次节 细胞内膜泡运输学问点二、了解包被蛋白种类学问点四、了解膜泡的转运、锚定与融合学问点五、了解细胞构造体系的组装Unit4Chapter 7 Cytosol and Endomembrane SystemSection 1 Cytoplasmic Matrix (Cytosol) and its functionsPoint 1 Apprehend the meaning of cytoplasmic matrixPoint 2 Master functions of cytoplasmic matrix

31、Section 2 Endomembrane System and Its FunctionPoint 1 Apprehend and master protein synthesis, modification and stress response in ER Point2ApprehendandmasterthepolarityofGolgicomplexanditsrolesinproteinsecretion,modification and proteolysisPoint 3 Apprehend and master lysosoms types, functions, biog

32、enesis, and its role in diseases Point4Knowthedifferenceoflysosomeandperoxisome,andthefunctionsandbiogenesisofperoxisomeUnit5Chapter 8 Protein Sorting and Vesicular TransportingSection 1 Protein SortingPoint 1 Apprehend and master signal hypothesis and sorting signal Point2Apprehendandmasterpathways

33、andtypesofproteinsortingPoint 3 Know protein sorting into mitochondrion, chloroplast, and peroxisomeSection 2 Vesicular Transporting in the CellPoint 1 Apprehend and master relations of structure and function for endomembrane organellesPoint 2 Know the types of coating proteinsPoint 3 Apprehend the

34、roles of 3 types of coating proteins in vesicular assembly andtransportingPoint 4 Know the process of vesicular transporting, docking and fusionPoint 5 Know the assembly of subcellular structures6 第九章 细胞信号转导3第一节 细胞信号转导概述学问点一、了解细胞通信类型其次节 细胞内受体介导的信号转递学问第三节、G 蛋白偶联受体介导的信号传递G 蛋白偶联受体介导的信号通路信号通路PI3K-PKB 信号

35、通路学问点三、了解其他类型信号通路第五节 其他细胞外表受体介导的信号通路信号通路NF-kB 信号通路Notch信号通路第六节 细胞信号转导的整合与把握学问点二、理解蛋白激酶的网络整合信息学问点三、理解并把握信号的把握：受体脱敏与下调Unit6Chapter 9 Cell Signal Transduction Section 1 Overview of Cell SignalingPoint 1 Know types of cell signalingPoint 2 Master types of signal molecules and receptorsPoint 3 Apprehend

36、and master characteristics of signal transductionSection2SignalTransductionbyIntracellularReceptorsPoint 1 Know nuclear receptors and functionsPoint 2 Master signal transduction by NOSection 3 Signal Transduction by G Protein-Coupled ReceptorsPoint 1 Apprehend and master the domain and activation of

37、 G protein-coupledreceptorsPoint 2 Apprehend signaling pathways mediated by G protein-coupled receptorsSection 4 Signal Transduction by Enzyme-coupled ReceptorsPoint 1 Apprehend the receptor tyrosine kinase and RTK-Ras signal pathwayPoint 2 Apprehend signal pathway mediated by PI3K-PKB(Akt)Point 3 K

38、now other types of signal pathwaysSection 5 Signal Pathways by Other Receptors on Cell SurfacePoint 1 Know the Wnt-beta-catenin signaling pathwayPoint 2 Know the Hedghog signaling pathwayPoint 3 Know the NF-kB signaling pathwayPoint 4 Know the Notch signaling pathwayPoint 5 Know signaling pathway me

39、diated by integrinsSection 6 The Convergence and Regulation of Signal TransductionPoint 1 Apprehend and master the features of signal responses Point2ApprehendtheConvergenceofthecross-talkofsignalingPoint 3 Apprehend the desensitization and down-regulation of receptors学问块三 细胞骨架系统Learning Block 3The

40、Cytoskeleton System学问单元一 第十章 细胞骨架4学问点一、理解并把握微丝装配动态性学问点三、把握微丝的分子马达种类学问点四、理解并把握肌肉收缩的分子机制学问点二、把握微观组织者中心类型学问点五、把握纤毛和鞭毛的构造、功能及运动机制学问点一、了解中间丝的类型学问点三、把握中间丝功能Unit1Chapter 10 The CytoskeletonSection 1 Microfilaments and Cell MotilityPoint 1 Apprehend and master the dynamic assembly of microfilamentsPoint 2 A

41、pprehend and master the types and functions of microfilament-associatedproteins and cell motilityPoint 3 Master the types of microfilament associated motor proteinsPoint 4 Apprehend and master molecular mechanism of muscle contractionSection 2 MicrotubulesPoint 1 Apprehend and master the polarity an

42、d dynamic properties of microtubulesPoint 2 Master types of microtubule-organizing centersPoint 3 Apprehend and master the regulation of microtubules by microtubule-associatedproteinsPoint 4 Apprehend and master motor proteins and intracellular transportPoint 5 Master the structure, function and mot

43、ility mechanism of cilia and flagellaSection 3 Intermediate FilamentsPoint 1 Master types of intermediate filamentsPoint 2 Master the assembly and disassembly and tissue distribution of intermediate filamentsPoint 3 Master the function of intermediate filamentsLearning Block 4 Genetic Information Ca

44、rrier and Expression System学问单元一 第十一章 细胞核与染色质(5学时)第一节 核被膜学问点三、把握核纤层功能学问点一、把握染色质的概念及化学组成学问点三、理解染色质包装的构造模型第三节 染色质的复制与表达学问点一、了解染色质复制与修复 学问点四、了解染色质与表观遗传学问点一、了解中期染色体的形态构造学问点三、了解核型与染色体显带第五节 核仁与核体学问点一、把握核仁的超微构造 学问点三、了解核仁的周期学问点四、了解核体Unit1Chapter 11 Nucleus and ChromatinsSection1NuclearEnvelopePoint 1 Master

45、 structure and dynamics of nuclear envelopePoint 2 Apprehend and master functions of nuclear pore complexPoint 3 Master the function of nuclear laminaSection2ChromatinPoint 1 Master concept and chemical composition of chromosome Point 2 Master the basic structure unit of chromosomenucleosomePoint 3

46、Apprehend structural model of chromosome packagePoint 4 Master of euchromatin and heterochromatinSection 3 Replication and Expression of ChromatinsPoint 1 Know the replication and repairmen of chromatins Point 2 Know the activation and inactivation of chromatins Point3Knowtheregulationofgeneexpressi

47、onbychromatinsSection4ChromosomesPoint 1 Know modal structure of metaphase chromosomePoint 2 Apprehend and master 3 functional elements of chromosome DNAPoint 3 Know karyotype and bandingPoint 4 Apprehend cause and function of giant chromosomeSection 5 The Nucleolus and The Nuclear BodyPoint 1 Maste

48、r ultrastructure of nucleolusPoint 2 Apprehend and master functions of nucleolusPoint 3 Know nucleolar cyclePoint 4 Know the nuclear bodies学问单元二 第十二章 核糖体1第一节 核糖体的类型与构造学问点二、把握核糖体的构造其次节 多聚核糖体与蛋白质的合成学问点一、了解多聚核糖体 学问点三、了解 RNA 在生命起源中的地位Unit2Chapter11Ribosome(Self-study)Section 1 Types and Structure of Rib

49、osome Point 1 Master basic types and composition of ribosomePoint 2 Master structure of ribosomePoint 3 Master functions ribosomal proteins and rRNASection2PolyribosomeandProteinSynthesisPoint 1 Know polyribosome Point2MasterproteinsynthesisPoint 3 Know position of RNA in life originLearning Block 5

50、 Cell Proliferation, Differentiation and CellSenescence and Apoptosis学问单元一 第十三章 细胞周期与细胞分裂 (3 学时)学问点二、把握细胞周期同步化的方法原理 学问点三、把握早期胚胎细胞的细胞周期学问点二、理解并把握动物细胞胞质分裂分子调控机制学问点四、把握减数分裂的特别构造及其变化Unit1Chapter 13 Cell Cycle and Cell DivisionSection 1 Cell CyclePoint 1 Master cell cycle concepts and cycling phrase even

51、ts Point 2 Master principle of cell cycle synchronization methodPoint 3 Master the cell cycle of the early frog embryoSection 2 Cell DivisionPoint 1 Apprehend and master events and apparatuses in cell cycle phasesPoint 2 Apprehend and master the mechanism of animal cytokinesis Point 3 Apprehend the

52、process and significances of meiosisPoint 4 Master the special apparatus and its dynamic of meiosis学问单元二 第十四章 细胞增殖调控与癌细胞 (3 学时)学问点一、理解 MPF 的觉察及其作用MPF学问点三、把握周期蛋白的作用学问点四、把握 CDK 激酶和 CDK学问点五、理解并把握细胞周期如何正常有序的运转及调控 学问点一、把握癌细胞的根本特征学问点三、理解肿瘤发生是基因突变积存的结果学问点四、理解并把握肿瘤干细胞Unit2Chapter 14 Cell Cycle Control and C

53、ancer CellsSection 1 Cell Cycle ControlPoint 1 Apprehend the discovery and functions of MPFof P 34cdc2 kinase and its relation with MPFPoint 3 Master functions of cyclic proteinPoint 4 Master CDK kinase and CDK inhibitorsPoint 5 Master and apprehend how cell cycle function properly and its regulatio

54、nPoint 6 Know other inner and outer factors in cell cycle regulationSection2CancerCellPoint 1 Master basic features of cancer cellsPoint 2 Master relations between cancers and oncogenes and tumor-suppressor genesPoint 3 Apprehend cancers results from accumulation of gene mutationPoint 4 Apprehend an

55、d master cancer stem cells学问单元三 第十五章 细胞分化与胚胎发育 (3 学时)学问点一、把握细胞分化的根本概念以及去分化和再生学问点三、了解影响细胞分化的因素学问点一、了解生殖细胞的分化学问点二、了解早期胚胎发育过程中的细胞分化Unit3Chapter15CellDifferentiationandEmbryonicDevelopmentSection 1 Cell DifferentiationPoint 1 Master basic concepts of cell differentiation, dedifferentiation and re-differ

56、entiationPoint 2 Know factors influencing cell differentiationPoint 3 Apprehend and master totipotency and stem cell Point4KnowcelldifferentiationandembryodevelopmentSection2CellDifferentiationinEmbryonicDevelopmentPoint 1 Know the differentiation of germ cellsPoint 2 Know the cell differentiation d

57、uring early embryonic development学问单元四 第十六章 细胞死亡与细胞年轻(3学时)第一节 细胞死亡学问点一、把握细胞死亡类型学问点二、理解并把握细胞凋亡的概念学问点三、把握细胞凋亡的形态学、生物化学特征及检测方法学问点四、理解细胞凋亡的生理学及医学意义学问点五、理解并把握细胞凋亡的分子机制及主要凋亡通路 学问点七、理解自噬性细胞死亡的分子机制其次节 细胞年轻Hayflick学问点三、理解并把握细胞年轻的分子机制：端粒学说和氧化损伤学说Unit4Chapter 15 Cell Death and Cell SenescenceSection1Cell DeathPoint 1 Master the types o