自动化产品线-核酸提取专题

1、LST 自动化产品线2015核酸专题王中禹2015.04什么是自动化产品？自动化产品有哪些价值？我们的自动化产品有哪些（三大系列）？我们有哪些竞争对手？What is Lab Automation?3加样机械臂移液分液工具工作台面（摆放样品管/试剂/tip等）控制电脑及软件抓扳手台面功能模块温控（水浴.金属浴）振荡（vortex）抽滤（离心机）磁珠分离器Plate:HandlerStorage（存放tip、样品板）仪器室（摆放大型仪器如MMD、PCR/QPCR/GX/HCS等）什么是自动化产品？Automated Liquid Handling Workstation顾名思义自动化（autom

2、ation）：是指在实验室中某些工作不需要实验工作者而是以无人值守的方式通过仪器设备来完成。液体处理（Liquid Handling）：在生命科学领域的研究中，几乎所有实验都是通过对液体进行操作来完成的，具体操作包括：吸液，排液，混合，转移等等。工作站（Workstation）：做实验的地方或做某种实验特定步骤的空间，一个工作平台，具体比如：实验台，无菌台，暗室，冷室，无菌室等。自动化液体处理工作站 自动完成 对液态实验品的系列操作 的工作平台。提问：什么叫自动化核酸提取工作站？自动化产品有哪些价值？标准化重复性精准度高通量自动化我们的自动化产品有哪些？（老PE自动化）NGS ExpressJ

3、ANUS系列JANUS Chemagic我们的自动化产品有哪些？（Caliper自动化）2007 Zephyr灵活型2009 Zephyr MBW2011 Zephyr NGS Sciclone NGSChemagen核酸提取仪Chemagen核酸提取仪有两款：chemagic 360可以从血液、血清/血浆、拭子、唾液、排泄物等多种样本中制备核酸，是核酸提取的完美自动化解决方案。chemagic 360chemagic Prepitochemagic Prepito采用chemagen磁棒微珠分离技术，是一台处于顶尖水平的桌面型样本制备设备。自动化产品发展理念的变革配置组合型：根据应用要求，组

4、合各种功能模块，通过各种必要配件的合理搭配，最终设计成满足客户应用的机型。应用型：解决典型实验需求的专用型工作站，按照市场上最有需求的，未来最有潜力的应用方向，专门为此应用设计出来单货号产品。配置组合型工作站JANUS系列JANUS 系列 JANUS Mini MDT JANUS Expanded Deck with Gripper Integration Platform JANUS Standard DeckJANUS Integrator JANUS Mini VarispanJANUS Expanded Deck with MDT JANUS Mini MDT with PlateSt

5、ak Mini 9 Deck Position Standard 24 Deck Position Integrator 24-32 Deck Position Expanded 32 Deck Position 4 8 Tips Varispan Arm 2 Arm Integration with external device 96 - 384 Tips MDT ArmVersaTipTechnology 0.5L 5mLTile - Concept4 Deck capacity3 Arm configurationMDT TechnologyDynamic volume rangefr

6、om 2nL (PinTool)to 235 uL (96Tips P235)JANUSwith Varispanfrom Vialsto Tubesto PlatesEnables multi-tipped processing regardless of labware Automatic variable tip spacing9 to 20 mm (4 tips)9 to 40 mm (8 tips)Tips automatically process sequentially if outside of Varispan limits with no additional progr

7、amming各移液通道可独立控制+间距可调以适应不同的液体处理formate及实验器具VersaTip PlusOn a Single Adapter Disposable Tips Fixed, Washable TipsUse Disposable tips to ensure zero carry-overUse fixed, washable tips for buffer or reagent additionNo changing of hardware, no user-intervention required自由切换使用固定钢针或一次性吸头根据所处理液体性质平衡防污染与降成本

8、AccuSense Liquid Level SensingAble to sense ionic, and non-ionic liquids including organic solvents such as DMSO or methanolHigh, medium, or low sensitivity settingsRequires minimal liquid in labwareLess than 100 L in a tubeLess than 50 L in a microplate wellIndependent liquid level sensing on each

9、tip for optimal precision and minimal sample carry-overJANUS MDT Head96通道移液头:I235/96 MDT P235吸头，5-235 ul，50ul:CV1%I50/96 MDT， P50吸头，0.5-50ul，5ul：CV1% P20吸头，0.5-20ul，1ul：CV2%384通道移液头I30/384 MDT P30吸头，0.5-30ul，1ul：CV5% P10吸头，0.5-10ul，1ul：CV5%NanoHead：细钢针，50nl 1ul，500nl：CV5%Pin tool专利技术自动在桌面上装载/卸载不同的移液

10、头，完全软件控制可选配MDT Gripper（装在MDT臂上的小型抓取机械手）做抓取动作MDT 专利技术JANUS with Gripper Integration PlatformGripper capable of moving plates around deck and to/from peripheral devices such as:Magnetic bead seperator / Vacuum manifoldReadersWashersIncubatorsand much moreComplete assay automation from sample preparati

11、on to results generationIntegrated or Auxiliary Gripper arm optionsGripper Specifications:Top GripperClose to GripOpen to Grip (Protection Pins)355 Rotational CapabilityJANUS: Modular Deck DesignFlexible deck layoutRotate labware at any timeEasier protocol definition and programmingLabware portabili

12、ty across deck and between instrumentsModular Deck DesignThe Tile ConceptJANUS: 功能模块 JANUS Liquid Handling SoftwareJANUS SoftwareDual Purpose GUIWinPREP Protocol Set-UpOpen & IntuitiveFull ControlKey / Power UsersJANUS Application AssistantRun ProtocolsVery Intuitive GuidanceNo BarriersNo Settings A

13、djustmentsMulti-User EnvironmentWinPREP 4.8 Programming InterfaceDeck Grid and Labware OverviewProtocol Tree and SpecificationsQuick Buttons for Intuitive ProgrammingRight mouse click:Add labware on deck (predefined labware library)Flush and Wash StationFlush and Wash StationReagent TroughFlush and

14、Wash StationReagent Trough96-well PlateJANUS Application Assistant User InterfaceCan be even converted into CHINESESelect Protocol From ListAnswer Protocol Related Questions123Shelf X Y Z4Gather Required Labware with HELP on. Item and Visual Quantity Needed Lab Location Purchasing LinkPlace Labware

15、on DeckCheck the ListProtocol Progress: follow the stepsStatus VisualColor CodedTime Status showing Start and Estimated CompletionTo Ensure a FRESH START All Over配置组合型工作站Cell Explorer上海东方肝胆医院睿智化学中国科学院动物所北京海克维尔有限公司应用领域分子生物学PCR Set-upDNA浓度归一化核酸提取及纯化基因组学NGS 库制备测序反应前处理多重基因表达蛋白质组学蛋白纯化MALDI点靶胶内酶解蛋白生物标记物分析

16、药物筛选样品处理化合物稀释板复制化合物库管理细胞分析高内涵筛选RNAi文库筛选GPCR分析细胞代谢检测临床应用分子诊断内分泌学检测感染性疾病检测免疫学检测医学检测血凝检测食品检验血样分析JANUS自动化工作站是一自动化液体处理系统，对基因组学、蛋白质组学、分子诊断、药物研发、法医学、化学等领域实验操作和常规液体处理等实现自动化。应用型工作站 NGS（第二代测序）NGS Express2011 Zephyr NGS Sciclone NGS专门为NGS应用而生的硬件设计预置NGS流行应用方案的金标准程序LabChip XT System Automated DNA Fractionation a

17、nd Sizing (low throughput, high accuracy)Zephyr NGS Workstation LabChip GX (Touch) System HT Bioanalyzer-like QC for gDNA, DNA and RNA, ng & pg sensitivitySciclone NGS Workstation Automated High-throughput Sequencing Sample PreparationMBWJANUS & chemagenDSXTGXNGSXTGXNGSWGeospizaZephyr Molecular Biol

18、ogy Workstation (MBW) JANUS Solutions and Chemagen Nucleic Acid ExtractionLabChip DS System UV-Vis DNA/RNA QuantificationSequencing ServiceBENEFITS:Smaller samples Reduced PCR biasGreater productivityInformatics correlation linkagesGeospiza LIMS Sample TrackingDiseaseResearchFragment& SizingGenerate

19、LibraryQuantifySampleSequenceAnalyzeBetter quality Higher throughput Lower costsJANUS NGS Express Workstation 应用型工作站 分子生物学（核酸提取）2011 Zephyr MBW 灵活的样品体积可以处理 10 L 到 4 mL的样品体积简化的提取流程 独立的液体处理和提取流程安排：初始样品转移、浓度归一化和 PCR 反应建立应用程序。96 磁头每次可运行最多 192 个样品24 磁头每次可运行最多 48 个样品最高的核酸产量和纯度比其他自动化提取平台高出两倍的通量和高出四倍的产量与其他市售平台相比更低的总处理时间JANUS Chemagic 灵活多用