布鲁菌属-微生物教学课件

1、Medical MicrobiologyDepartment of Microbiology, HMU第二篇 致病性细菌第24章布鲁菌属Brucella 微生物学教研室 钟照华教授 商庆龙讲师 教学大纲掌握内容掌握主要生物学性状（抗原构造与分型） 熟悉内容熟悉布鲁菌的免疫性及微生物学检查法 了解内容了解布鲁菌的防治原则 问题布鲁菌抗原构造与分型？布鲁菌是如何致病的？布鲁菌引起的免疫反应如何？ 布鲁菌属 Brucella引起人类、家畜和其它动物布鲁菌病分6个生物种羊布鲁菌 B. melitensis牛布鲁菌 B. abortus猪布鲁菌 B. suis鼠布鲁菌 B. meotomae绵羊布鲁菌

2、B. ovis犬布鲁菌 B. canis生物学性状革兰阴性小杆菌无鞭毛，不形成芽胞，光滑型菌株有微荚膜 专性需氧生长缓慢分解葡萄糖，产生少量酸过氧化氢酶阳性，氧化酶阳性产生H2SB. melitensis分泌物、乳品消化道、呼吸道粘膜、皮肤及眼结膜人菌血症16W潜伏期（70）发热、出汗、关节痛和全身乏力内毒素进入肝、脾、骨髓（发热消退）繁殖入血菌血症（体温再次上升）波浪热致病性致病物质内毒素、透明质酸酶、荚膜所致疾病母畜流产赤藓醇胎盘、绒毛膜含量高Brucella species SummaryBiologySmall gram-negative rods without capsuleObl

3、igatory aerobicGrows slowly in enriched culture media with 10% CO2H2S + Pathogens Human pathogen Animal reservoirsBrucella melitensis goat, sheepBrucella abortus cattleBrucella suis pigTransmissionEnter human body by Ingestion of contaminated milk productsThrough the skin directly contact in an occu

4、pational setting such as abattoir Disease: BrucellosisAnimalAbortionHumanWeakness and fatigueEnlarged lymph nodes, liver, and spleenUndulant fever (rising-and-falling fever)Occurs in a few patientsDiagnosisIsolationSample: blood, bone marrowSerological examinationAgglutination testComplement fixation testCoombs test Skin testPrevention & TherapyPasteurization of milkImmunizationAnimalsOccupational high-risk personsSlaug