Tomatidine - NF-κB 抑制剂 - 生命科学试剂 - MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemETomatidineCat. No.: HY-N2149CAS No.: 77-59-8分式: CHNO分量: 415.65作靶点: NF-B; JNK作通路: NF-B; MAPK/ERK Pathway储存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性数据体外实验 DMSO : 2.86 mg/mL (6.88 mM; Need ultrasonic)H

2、2O : 40% PEG300 5% Tween-80 45% salineSolubility: 0.29 mg/mL (0.70 mM); Clear solution2. 请依序添加每种溶剂： 10% DMSO 90% (20% SBE-CD in saline)Solubility: 0.29 mg/mL (0.70 mM); Clear solution3. 请依序添加每种溶剂： 10% DMSO 90% corn oil1/2 Master of Small Molecules 您边的抑制剂师www.MedChemESolubility: 0.29 mg/mL (0.70 mM);

3、 Clear solutionBIOLOGICAL ACTIVITY物活性 Tomatidine 通过阻断 NF-B 和 JNK 信号发挥抗炎作。IC50 & Target p65 JNK体外研究 Tomatidine decreases inducible NO synthase and COX-2 expression through suppression of I-Bphosphorylation, NF-B nuclear translocation and JNK activation, which in turn inhibits c-jun phosphorylationand

4、 Oct-2 expression. Tomatidine, solasodine and diosgenin (40 M) show 66%, 22% and 41% inhibition ofnitrite production, respectively. The iNOS protein is barely detectable in unstimulated cells but markedlyincreases after LPS treatment, and Tomatidine causes dose-dependent inhibition of LPS-induced iN

5、OSexpression. p65 is the major component of NF-B in LPS-stimulated macrophages, the effect of Tomatidineon p65 DNA-binding activity is determined. In the presence of Tomatidine at 10-40 M, the binding activity ofNF-B is suppressed in a dose-dependent manner. Tomatidine inhibits the phosphorylation o

6、f I-B, blocksthe I-B production, and furthermore suppresses p65 NF-B translocation to the nucleus and modulatedbinding activity 1.PROTOCOLCell Assay 1 RAW 264.7 cells, derived from murine macrophages, are cultured in DMEM supplemented with 10%endotoxin-free, heat-inactivated fetal calf serum, Penici

7、llin (100 units/mL), and Streptomycin (100 g/mL) in a5% CO2 atmosphere at 37C in a humidified incubator. For all assay, cell is plated at 2105 cells/cm2 inculture dishes or plates. Treatment with vehicle (0.1% DMSO or 0.1% ethanol), test compounds and/or LPSis carried out under serum-free conditions

8、 1.MCE has not independently confirmed the accuracy of these methods. They are for reference only.户使本产品发表的科研献 FASEB J. 2019 Feb;33(2):2574-2586.See more customer validations on HYPERLINK / www.MedChemEREFERENCES1. Chiu FL, et al. Tomatidine inhibits iNOS and COX-2 through suppression of NF-kappaB and JNK pathways in LPS-stimulated mousemacrophages. FEBS Lett. 2008 Jul 9;582(16):2407-12.McePdfHeightCaution: Product has not been fully validated for medical applica