DFHBI - Dye Reagent - 生命科学试剂 - MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEDFHBICat. No.: HY-110250CAS No.: 1241390-29-3分式: CHFNO分量: 252.22作靶点: Others作通路: Others储存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性数据体外实验 DMSO : 83.33 mg/mL (330.39 mM)* means soluble, but saturation

2、unknown.Mass Solvent1 mg 5 mg 10 mg Concentration制备储备液1 mM 3.9648 mL 19.8240 mL 39.6479 mL5 mM 0.7930 mL 3.9648 mL 7.9296 mL10 mM 0.3965 mL 1.9824 mL 3.9648 mL请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液，并请注意储备液的保存式和期限。体内实验请根据您的实验动物和给药式选择适当的溶解案，配制前请先配制澄的储备液，再依次添加助溶剂(为保证实验结果的可靠性，体内实验的作液，建议您现现配，当天使；澄的储备液可以根据储存条件，适当保存

3、；以下溶剂前的百分 指该溶剂在您配制终溶液中的体积占)：1. 请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% salineSolubility: 1.43 mg/mL (5.67 mM); Clear solution2. 请依序添加每种溶剂： 10% DMSO 90% (20% SBE-CD in saline)Solubility: 1.43 mg/mL (5.67 mM); Clear solution3. 请依序添加每种溶剂： 10% DMSO 90% corn oil1/2 Master of Small Molecules 您边的抑制剂

4、师www.MedChemESolubility: 1.43 mg/mL (5.67 mM); Clear solutionBIOLOGICAL ACTIVITY物活性 DFHBI种类似于绿荧光蛋 (GFP) 发团的分。Spinach 和 DFHBI 在未结合时 本不发荧光，Spinach-DFHBI 复合物在体外和活细胞中都具有明亮的荧光。体外研究 These RNAs interact with DFHBI to produce a bluish-green fluorescence emission (501 nm) after excitationat 447 nm. Spinach a

5、nd Spinach2 are RNA aptamers that can be used for the genetic encoding offluorescent RNA. Spinach2 binds and activates the fluorescence of DFHBI, allowing the dynamiclocalizations of Spinach2-tagged RNAs to be imaged in live cells. The spectral properties of Spinach2 arelimited by DFHBI, which produ

6、ces fluorescence that is bluish-green and is not optimized for filters commonlyused in fluorescence microscopes. Spinach and Spinach2 bind to DFHBI have fluorescence excitationmaxima of 447 nm and peak fluorescence emission of 501 nm 1. Broccoli-tagged RNAs are selectivelydetected in total cellular

7、RNA by gel electrophoresis followed by staining of gels with DFHBI, the Broccoli-binding fluorophore. Spinach is a 98-nt-long RNA aptamer that binds to and switches on the fluorescence ofDFHBI. Both Spinach and DFHBI are essentially nonfluorescent when unbound, whereas the Spinach-DFHBIcomplex is br

8、ightly fluorescent both in vitro and in living cells. DFHBI should be shielded from light. All stocksolutions of DFHBI should be maintained in dark tubes or wrapped in foil. Plates containing culturesincubated with DFHBI should be kept in the dark by using a foil overwrap 2.REFERENCES1. Song W, et a

9、l. Plug-and-play fluorophores extend the spectral properties of Spinach. J Am Chem Soc. 2014 Jan 29;136(4):1198-201.2. Strack RL, et al. Using Spinach-based sensors for fluorescence imaging of intracellular metabolites and proteins in living bacteria. NatProtoc. 2014 Jan;9(1):146-55.McePdfHeightCaution: Product has not been fully validated for medica