6-Gingerol-S-plus-6-Gingerol-DataSheet-生命科学试剂-MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemE6-GingerolCat. No.: HY-14615CAS No.: 23513-14-6Synonyms: (S)-(+)-6Gingerol; 6-Gingerol分式: CHO分量: 294.39作靶点: AMPK; Apoptosis作通路: Epigenetics; PI3K/Akt/mTOR; Apoptosis储存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C

2、1 month溶解性数据体外实验 DMSO : 50 mg/mL (169.84 mM)* means soluble, but saturation unknown.Mass Solvent1 mg 5 mg 10 mg Concentration制备储备液1 mM 3.3969 mL 16.9843 mL 33.9685 mL5 mM 0.6794 mL 3.3969 mL 6.7937 mL10 mM 0.3397 mL 1.6984 mL 3.3969 mL请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液，并请注意储备液的保存式和期限。体内实验请根据您的实验动物和给药式选择适当

3、的溶解案，配制前请先配制澄的储备液，再依次添加助溶剂(为保证实验结果的可靠性，体内实验的作液，建议您现现配，当天使；澄的储备液可以根据储存条件，适当保存；以下溶剂前的百分 指该溶剂在您配制终溶液中的体积占)：1. 请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% salineSolubility: 2.5 mg/mL (8.49 mM); Clear solution2. 请依序添加每种溶剂： 10% DMSO 90% (20% SBE-CD in saline)Solubility: 2.5 mg/mL (8.49 mM); Clear solut

4、ion1/3 Master of Small Molecules 您边的抑制剂师www.MedChemE3. 请依序添加每种溶剂： 10% DMSO 90% corn oilSolubility: 2.5 mg/mL (8.49 mM); Clear solutionBIOLOGICAL ACTIVITY物活性 6-Gingerol从姜中分离的活性物质，具有多种物活性，包括抗癌，抗炎和抗氧化。IC50 & Target AMPK Apoptosis体外研究 6-gingerol inhibits colon cancer cell proliferation and induced apopt

5、osis, while the normal colon cells areunaffected. 6-gingerol down-regulates phorbol myristate acetate induced phosphorylation of ERK1/2 andJNK MAP kinases and activation of AP-1 transcription factor, but has only little effects on phosphorylation ofp38 MAP kinase and activation of NF-kappa B 1. 6-gi

6、ngerol treatment is shown to restore impairedintestinal barrier function and to suppress proinflammatory responses in DSS-treated Caco-2 monolayers.AMPK is activated on 6-gingerol treatment 2. Treatment with 6-gingerol results in a significant decreasein the viability of osteosarcoma cells in a dose

7、-dependent fashion. In parallel, the number of cells arrested atthe sub-G1 cell cycle phase is significantly increased. 6-gingerol induces activation of caspase cascadesand regulates cellular levels of Bcl2 and Bax 3.体内研究 In animal studies, 6-gingerol significantly ameliorates DSS-induced colitis by

8、 restoration of body weight loss,reduction in intestinal bleeding, and prevention of colon length shortening. In addition, 6-gingerolsuppresses DSS-elevated production of proinflammatory cytokines (IL-1, TNF, and IL-12) 2.PROTOCOLCell Assay 1 6-gingerol stock (20 mg/mL) is prepared in ethanol and th

9、e working concentrations are prepared by dilutingthis stock in dimethyl sufoxide (DMSO). For MTT assay, 5103 cells/well of human colon cancer cells and104 cells/well of mouse IECs are seeded in 96-well plates. Cells are treated with 6-gingerol for 48 h,72 h or96 h before performing MTT assay and for

10、 16 h before Annexin-V staining 1.MCE has not independently confirmed the accuracy of these methods. They are for reference only.Animal Mice: Mice with DSS-induced colitis are given different oral dosages of 6-gingerol daily for 14 days. BodyAdministration 2 weight and colon inflammation are evaluat

11、ed, and level of proinflammatory cytokines in colon tissues ismeasured 2.MCE has not independently confirmed the accuracy of these methods. They are for reference only.户使本产品发表的科研献 PLoS Biol. 2018 Jul 12;16(7):e2004921.See more customer validations on HYPERLINK / www.MedChemEREFERENCES2/3 Master of S

12、mall Molecules 您边的抑制剂师www.MedChemE1. Radhakrishnan EK, et al. 6-Gingerol induces caspase-dependent apoptosis and prevents PMA-induced proliferation in colon cancercells by inhibiting MAPK/AP-1 signaling. PLoS One. 2014 Aug 26;9(8):e104401.2. Chang KW, et al. 6-Gingerol modulates proinflammatory resp

13、onses in dextran sodium sulfate (DSS)-treated Caco-2 cells andexperimental colitis in mice through adenosine monophosphate-activated protein kinase (AMPK) activation. Food Funct. 2015Oct;6(10):3334-41.3. Fan J, et al. 6-Gingerol inhibits osteosarcoma cell proliferation through apoptosis and AMPK activation. Tumour Biol. 2015Feb;36(2):1135-41.McePd