imagej使用分析荧光图片 (2)

1、Gao yan1ImageJ FeaturesRuns Everywhere: ImageJ is written in Java, which allows it to run on Linux, Mac OS X and Windows, in both 32-bit and 64-bit modes. Open Source: ImageJ and its Java source code are freely available and in the public domain. No license is required. Plugins: Extend ImageJ by dev

2、eloping plugins using ImageJs built in text editor and Java compiler. More than 500 plugins are available. Analysis: Measure area, mean, standard deviation, min and max of selection or entire image. Measure lengths and angles. Use real world measurement units such as millimeters. Calibrate using den

3、sity standards. Generate histograms and pro.2InstallationLinux Mac OS X Mac OS 9 Windows 3WindowsDownload ImageJ 1.44 bundled with 32-bit Java 1.6.0_20 (28MB), with 64-bit Java 1.6.0_20 (24MB; requires 64-bit Windows) or without Java (3MB). 4OpenReads an image and displays it in a separate window. F

4、iles must be in TIFF, GIF, JPEG, DICOM, BMP, PGM or FITS format. Also opens ImageJ and NIH Image lookup tables (with .lut extension). Additional are supported via plugins installed in the Import submenu. 56789Subtract background101112131415161718Merge channel19202122Scale barScale bars should be pre

5、sent on all publication/presentation images/movies. Its worth putting them in sooner rather than later. Choose a standard scale bar size for all your images if possible to avoid confusion.23If you know the size of a feature (previously applied scale bar for instance) you can use this command to appl

6、y a calibration.Using the line selection tool, draw a ling along the length of the feature/scale bar. Run the menu command “Plugins/Spatial calibration/Set scale”1. Enter the dimensions of the object/scale bar in the “known distance” box and set the units in the Unit of length box. Do not check Glob

7、al unless you wish all your images to have this calibration! Click OK. 242526272829Analyze Particles and count cellThis command counts and measures objects in binary or thresholded images. It works by scanning the image or selection until it finds the edge of an object. It then outlines the object using the wand tool, measures it using the Measure command, fills it to make it invisible, then resumes scanning until it reaches the end of the image or selection. Press the esc key to abort this