大豆苷元作用机制 - Medchemexpress - MCE中国

1、Product Data SheetDaidzeinCat. No.: HY-N0019CAS No.: 486-66-8分式: CHO分量: 254.24作靶点: PPAR; Endogenous Metabolite作通路: Cell Cycle/DNA Damage; Metabolic Enzyme/Protease储存式: 4C, stored under nitrogen* In solvent : -80C, 6 months; -20C, 1 month (stored under nitrogen)溶解性数据体外实验 DMSO : 50 mg/mL (196.66 mM)H2

2、O : 0.1 mg/mL (insoluble)* means soluble, but saturation unknown.SolventMass1 mg 5 mg 10 mgConcentration制备储备液1 mM 3.9333 mL 19.6665 mL 39.3329 mL5 mM 0.7867 mL 3.9333 mL 7.8666 mL10 mM 0.3933 mL 1.9666 mL 3.9333 mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存式和期限：-80C, 6 months; -20C,

3、 1 month (stored under nitrogen)。-80C 储存时，请在 6 个内使，-20C 储存时，请在 1 个内使。体内实验 请根据您的实验动物和给药式选择适当的溶解案。以下溶解案都请先按照 In Vitro 式配制澄清的储备液，再依次添加助溶剂：为保证实验结果的可靠性，澄 的储备液可以根据储存条件，适当保存；体内实验的作液，建议您现现配，当天 使； 以下溶剂前显的百分 指该溶剂在您配制终溶液中的体积占；如在配制过程中出现沉淀、析出现象，可 以通过加热和/或超声的式助溶1. 请依序添加每种溶剂： 10% DMSO 90% corn oilSolubility: 2.5 m

4、g/mL (9.83 mM); Clear solution此案可获得 2.5 mg/mL (9.83 mM，饱和度未知) 的澄 溶液，此案不适于实验周 期在半个以上的实验。以 1 mL 作液为例，取 100 L 25.0 mg/mL 的澄 DMSO 储备液加到 900 L 油中，混合均匀。BIOLOGICAL ACTIVITYPage 1 of 2 www.MedChemE物活性 Daidzein种异 酮，可作 PPAR 激活剂。IC & Target PPAR- PPAR- Human Endogenous Metabolite(批量添加)体外研究 In 3T3-L1 adipocytes

5、, Daidzein inverses the attenuation of adiponectin gene expression by co-culture, and theseeffects are inhibited by the PPAR- specific inhibitor. Daidzein attenuates the reduction of adiponectin expression inadipocytes, and a PPAR- specific inhibitor abrogated this effect. Direct activation of PPAR-

6、 and- by Daidzein isconfirmed by a luciferase reporter assay. In HEK293T cells, Daidzein significantly increases PPAR- transcriptionalactivity in a concentration-dependent manner. Although an obvious dose-dependency is not observed in PPAR-transcriptional activity, Daidzein also significantly increa

7、ses PPAR- transcriptional activity over a similar range ofconcentrations at which Daidzein enhanced PPAR- transcriptional activity, with a maximum increase at 25 M1.Daidzein is a soy isoflavone, which upregulates the expression of Abcg1, and it promotes axonal outgrowth incultured hippocampal neuron

8、s via estrogen receptor signaling. Daidzein is a major component of soy with structuralsimilarity to estrogen. It exerts an anti-inflammatory effect, lowers lipid levels, and increases mitochondrialbiogenesis. As an activator of nuclear receptor peroxisome proliferator-activated receptors (PPARs), D

9、aidzeinenhances transcription of PPARs-dependent genes, including liver X receptors (LXRs, Nr1h gene family in mice).Incubation with different concentrations of Daidzein, from 5 to 100 M, increases APOE transcriptional activity2.体内研究 Treating Apoe KO mice with Daidzein increases Lxr and Abca1 gene e

10、xpression at 1 month after stroke, showingthat the absence of ApoE does not interfere with other cholesterol homeostasis genetic programs. Therefore, thefindings suggest that Daidzein-induced ApoE upregulation is a critical component in fostering functional recovery inchronic stroke2.PROTOCOLCell As

11、say 1 HEK293T cells are plated on 24-well plates at a cell density of approximately 2.5104 cells/well and are grown to 70-80% confluence. Cells are then transiently transfected with a PPAR- or PPAR- expression plasmid, and a plasmidcontaining the luciferase gene under the control of three tandem PPA

12、R response elements (PPRE 3 TK-luciferase)using an X-treme GENE HP DNA Transfection Reagent. Renilla luciferase control vectors are co-transfected to controlfor transfection efficiency. After transfection, cells are cultured for another 24 h in medium containing DMSO orvarious concentrations (6.25,

13、12.5, 25 M) of Daidzein. Cells are lysed, and luciferase activity is measured andexpressed as fold induction, that is normalized to the activity of the renilla luciferase control plasmid1.MCE has not independently confirmed the accuracy of these methods. They are for reference only.Animal Mice2Admin

14、istration 2 Experiments are performed in 10- to 11-week-old male C57 (C57 bl/6) and Apoe KO (C57 background) mice. Forlong-term stroke recovery, mice receive Moxifloxacin (100 mg/kg) for 3 d. The prophylactic antibiotic treatment isshown to effectively reduce mortality in an animal model of stroke b

15、y attenuating peripheral infection. In addition,saline is subcutaneously administered daily, and hydrogel (Clear H2O) is given to prevent dehydration. With theimplementation of poststroke care (antibiotic regimen, rehydration, and feeding hydrogels with soft diet) during theacute period (1 week), mi

16、ce start to regain their body weight by day 5 and continue to recover from stroke. Animalsare randomly selected for vehicle or Daidzein treatment. Vehicle or Daidzein (10 mg/kg) is administeredsubcutaneously within 30 min of reperfusion after confirming the reperfusion of blood flow, daily for 7 d a

17、nd thenevery other day up to 1 month.MCE has not independently confirmed the accuracy of these methods. They are for reference only.户使本产品发表的科研献Page 2 of 3 www.MedChemE Microchem J. 2020 Feb.See more customer validations on HYPERLINK www.MedChemE www.MedChemEREFERENCES1. Sakamoto Y1, et al. The Dietary Isoflavone Daidzein Reduces Expression of Pro-Inflammatory Genes through PPAR/ and JNK Pathways in Adipocyteand Macrophage Co-Cultures. PLoS One. 2016 Feb 22;11(2):e0149676.2. Kim E, et al. Daidzein Augments Cholesterol Homeostasis via ApoE to Promote Functional Recovery in Chron