常用溶液的配制方法

1、常用溶剂的配制方法1磷酸缓冲液：0.15M,pH=7.4磷酸缓冲液：KH2PO4:2.041g+100ml水K2HPO43出0：10.3g+300mL水两液混合即成400mL,0.15M,pH=7.4的磷酸缓冲液0.2mol/L不同pH的磷酸缓冲液：先配制0.2mol/L的磷酸二氢钾溶液和0.2mol/L的磷酸二氢钾溶液，然后按下表配制：pHKH2PO(mL)K2HPO4-3H2O(mL)pHKH2PO4K2HPO4-3H2O(mL)5.793.56.56.945.055.05.892.58.07.039.061.05.990.010.07.133.067.06.087.712.37.228.

2、072.06.185.015.07.323.077.06.281.018.57.419.081.06.377.522.57.516.084.06.473.526.57.613.087.06.568.531.57.710.590.06.662.537.57.88.591.56.756.543.57.97.093.06.851.049.08.05.394.72硼酸缓冲液0.15M,pH=8.2硼酸缓冲液：四硼酸钠溶液：2g+35mL水硼酸溶液：3.246g硼酸+350mL水两液混合即成700mL,0.15M,pH=8.2的硼酸缓冲液0.2mol/L(硼酸根)，不同pH的硼酸缓冲液：先配制0.2mo

3、l/L的硼酸溶液和0.05mol/L的四硼酸钠溶液，然后按下表配制：pH四硼酸钠mL硼酸mLpH四硼酸钠mL硼酸mL7.41.09.08.23.56.57.61.58.58.44.55.57.82.08.08.76.04.08.03.07.09.08.02.03.甘氨酸盐酸缓冲液：0.2mol/L0.2mol/L甘氨酸溶液(15.01g/L)pH0.2mol/L甘氨酸mL0.2mol/L盐酸mLpH0.2mol/L甘氨酸mL0.2mol/L盐酸mL2.05044.43.05011.42.45032.43.2508.22.65024.23.4506.42.85016.83.6505.04柠檬酸缓

4、冲液：O.1mol/LC6H8O7H2O:0.1mol/L溶液为21.01g/LNaaCeHsO?.2H2O：O.1mol/L溶液为29.41g/LpH0.1mol/L柠檬酸mL0.1mol/L柠檬酸钠mLpH0.1mol/L柠檬酸mL0.1mol/L柠檬酸钠mL3.018.61.45.08.211.83.217.22.85.27.312.73.416.04.05.46.413.63.614.95.15.65.514.53.814.06.05.84.715.34.013.16.96.03.816.24.212.37.76.22.817.24.411.48.66.42.018.04.610.39

5、.76.61.418.44.89.210.80.1MpH=4柠檬酸缓冲液222mLVi:柠檬酸V2：柠檬酸钠Vi=131mL0.1*0.131*210.14=2.75gV2=91mL0.1*0.091*294.10=2.68g0.1MpH=2.3柠檬酸缓冲液412mLVi:柠檬酸V2:柠檬酸钠Vi=400mL0.1*0.4*210.14=8.40gV2=12mL0.1*0.012*294.10=0.353g1MpH=10碳酸缓冲液211mLVi:碳酸钠V2:碳酸氢钠Vi=150mL1*0.15*106=15.9gV2=61mL1*0.061*84=5.12g原来1MpH=7.4磷酸缓冲液203

6、mLVi：K2HPO4-3H2OV2:KH2PO4Vi=150mL0.15*1*228.22=34.23gV2=53mL0.053*1*136.08=7.21g现在1MpH=7.4磷酸缓冲液134mLVi：K2HPO4-3H2OV2:KH2PO4Vi=100mL0.1*1*228.22=22.82gV2=34mL0.034*1*136.09=4.3g0.1MpH=8磷酸缓冲液20.15mLVi：K2HPO4-3H2OV2:KH2PO4Vi=20mL0.1*0.02*228.22=0.46gV2=0.15mL00*0.15*10-3*136.08=0.002g0.5MpH=8Tris-HCI缓冲

7、液165mLVi：TrisV2：HCIV仁105mL0.5*0.105*12.1.=6.36gV2=60mL(0.5MHCI)0.2MpH=4.3柠檬酸缓冲液372mLVi:柠檬酸V2：柠檬酸钠Vi=300mL0.3*0.2*210.14=12.61gV2=72mL0.2*0.072*294.10=4.24g0.2MpH=4柠檬酸缓冲液340mLVi:柠檬酸V2:柠檬酸钠Vi=200mL0.1*0.2*210.14=8.41gV2=140mL0.2*0。4*29910=8.23g5.Tris-HCI缓冲液:O.1mol/L100mL0.1mol/L三羟甲基氨基甲烷(Tris)溶液与一定量的。.

8、1mol/L盐酸混匀，可得O.1mol/L,不同pH的缓冲液。pH0.1mol/L盐酸(mL)pH0.1mol/L盐酸(mL)pH0.1mol/L盐酸(mL)7.289.47.869.08.434.47.386.87.964.08.529.47.484.08.058.48.624.87.580.68.152.48.720.67.677.08.245.88.817.07.773.28.339.88.914.0200mL0.1MTris(2.42g)加入0.1MHCI24mLpH=9,0.1MTris-HCIbuffer6.醋酸缓冲液:0.2mol/L0.2mol/L醋酸钠：27.22g三水醋酸钠

9、(无水的为16.4g)+1L水0.2mol/L醋酸:11.55mL冰醋酸+1L水pH醋酸钠mL醋酸mLpH醋酸钠mL醋酸mL3.60.759.254.85.904.103.81.208.805.07.003.004.01.808.205.27.902.104.22.657.355.48.601.404.43.706.305.69.100.904.64.905.105.89.400.607.碳酸缓冲液：0.1mol/L（Ca2+、Mg2+存在时不得使用）pH0.1mol/L碳酸钠(mL)0.1mol/L碳酸氢钾（mL）20C37C9.168.77199.409.12289.519.40379.7

10、89.50469.909.725510.149.906410.2810.087310.5310.288210.8310.57910.1mol/LMES缓冲液:1.921gMES+100mL水，pH=4.098.电泳溶液：电泳缓冲液：3gTris碱、14.4g甘氨酸和IgSDS溶于水中，调pH至8.3左右，加水定容至1L。丙烯酰胺溶液（49.5%T，3%C）:48g丙烯酰胺和1.5gbis溶于100mL重蒸水中。分离胶缓冲液（4X）:75ml2mol/LTris-HCI缓冲液（pH=8.8）+4ml0.1mol/LSDS+46mL重蒸水。浓缩胶缓冲液(4X):50ml2mol/LTris-HCI缓冲液(pH6.8)+4ml0.1mol/LSDS+46mL重蒸水2%前三酮溶液：50mL95%乙醇+1g荀三酮(避光，4C保存)2%乙醇胺溶液：2mL乙醇胺+98mL硼酸缓冲液(0.15M,pH=8.2)，调pH=8.22M醋酸溶液：114.4mL冰醋酸+去离子水定容至1L0.5mol/LEDTA:配制等摩尔的Na2EDTA和NaOH溶液(0.5mol/L)，混合后形成EDTA的三钠盐。或称取186.1g的NazEDTA2出0和20g的NaOH,并溶于水中，定容至1L考马斯亮蓝溶液：考马斯亮蓝G-250100mg溶