响应面优化法对香菇多糖提取的工艺研究_图文

1、 14辽宁农业科学2010年 图5Y=f(A,B的响应面图和等高线图Figure 5R皓ponsiVe surfac鹤andntou瑙of Y=f(A.B 图6Y=f(A。c的响应面图和等高线图Figure 6R唧。嘣ve锄rfaces ad咖tours of Y=f(A。C1 图7Y=f(B,c的响应面图和等高线图Figre 7Responsive surfhces andcotours of Y=f(B.C图5显示了A由高水平到低水平变化过程中等高线的数目明显多于B,说明浸提温度对香菇多糖得率的影响很显著。由图6可以看出,等高线呈椭圆形,且椭圆的轴线与坐标轴之间的有较大的角度,说明温度与料

2、液比之间存在显著的交互作用。对多元二次回归方程进行求偏导,可以得到:l,=1.060.013曰一6.5A+0.84C:l,=O.19一O.013A一0.42口+0.73C;y=0.60+O.84A+O.73日一3.92C联立方程组,解得热水浸提香菇多糖最优条件的编码值如下:A=0.138,B=0.002,C=0.169。即在浸提温度为78.6,浸提时间为120min,料液比为l:58.3时,模型预测香菇粗多糖得率最大值为14.12%。3结论通过BoxBenhnken设计和响应面分析法,利用DesignExperc统计软件能够可靠地进行优化实验以及数据处理。本实验应用这一方法对香菇多糖提取的工艺

3、条件进行了优化。在单因素实验的基础上,应用BoxBenhnken设计三因素三水平的实验,确定出香菇多糖提取的优化工艺条件为:提取温度78.6,提取时间120min和料液比1:58.3,提取得率为14.22%及其多糖含量为38.57%。参考文献:Ch蛐gsT.Mush删biology:theimp虬t口nmuBhroomp一duction蛆dmushmomproductBJ.Mu8hfoom Biology andMu8hroomProduct8.1993.122:320.王志明,陈祥贵.香菇营养成分的酶解提取及利用J.四JII工业学院学报,1998,17(1:4649.ZHOULD,zHANG

4、QH,ZHANGY.The 8hiitake mushrmderived immune-8timulant lentinanprot即ts醒ainstmurinema-ldablood-暑tage infec“onby evoking adaptive immune一咒Bpon-啷J.Int JImmunopha咖acol,2009,9(4:455462.Chiham G。Maeda YY,H8mur0J。SB8aki T,FumikoF.Inhibitionof mouseBarcomaI 80bypolysacchade8fmmI七ntinuBedode8J.Nalure,1969,222

5、:687688.Fue H,Kjtoh I.Ph粕e ll l一8tudy onLentinan.J印蛐e8eJ.CcerChemotheMpy。1981.8:944960.张惟杰.糖复合多糖生化研究技术(第二版M.杭州:浙江大学出版社,1994.Box G E P。Hunter W G.Stati8tics for Experiment8:柚IntrDduction toDeBign,DataAn8lysis朋dModelBuildingM.NewYork:Wiley.1990.Optimi2,ation of Extraction of Lentinan by Response Surfa

6、ce Method0109ywEl zhenyuanl,zHoNc Yao-guan91-,LIuchang-jian矿(1.Co岫e o,d sc函撇n以,loz啊,髓肌g口i D促a几踟咖睁,s们ghi201306;2.z妇e口厂d&如nce,sk吖。昭Ag廊曲umZ踟沁H妙,_sk,邺喈,“咖llOl61Abstract:Response surfacemethodology(RSMwa8appliedtooptimize the hotwaterextr屺tions of lentinan.Extmction temper.atu陀,ex帆ction time and solid-“q

7、uid mtio we他selected鹅causal facto鸭.Basedonthesine faeto埔experiments,a tIlree.factor.tlIreeleveI exPeriments designweredeVeloped by BoxBenhnken centr8l comp08ite de8ign method.The yield of polysaccharide8wused舶the respon8ive valueB.The optimum hotwaterextraction conditions were船follow:extmction tempe

8、mtu咒i878.6、extmction time is 120minand tlIe 80lid-liquid is 1:58.3(g/m1.Under the optimum extraction technology condition,出e yieldof polysaccharides is 14.22%and the contend of polvsaccharides is 38.57%.KeywoHls:Lentin舳;ResponBe蛐dhce methodology;Extnction;0ptimiza“吲 响应面优化法对香菇多糖提取的工艺研究作者:魏桢元, 钟耀广, 刘长

9、江作者单位:魏桢元(上海海洋大学食品学院,上海,201306, 钟耀广(上海海洋大学食品学院,上海,201306;沈阳农业大学食品学院,辽宁,沈阳,110161, 刘长江(沈阳农业大学食品学院,辽宁,沈阳,110161刊名:辽宁农业科学英文刊名:LIAONING AGRICULTURAL SCIENCES年,卷(期:2010(2参考文献(7条1.Box G E P;Hunter W G Statistics for Experiments:an Introduction to Design,Data Analysis and Model Building 19902.张惟杰糖复合多糖生化研究技

10、术 19943.Furue H;Kitoh I Phase 111-study on Lentinan.Japanese 19814.Chihara G;Maeda YY;Hamuro J;Sasaki T,Fumiko F Inhibition of mouse sarcoma 180 by polysaccharides from Lentinus edodes 19695.ZHOU LD;ZHANG QH;ZHANG Y The shiitake mushroom-derived immune-stimulant lentinan protects against murine malaria blood-stage infection by evokin