NEB保护碱基-各种酶切位点保护碱基(共4页)

1、精选优质文档-倾情为你奉上PCR设计引物时酶切位点的保护酶寡核苷酸序列切割率%2 hr20 hrAcc IGGTCGACC CGGTCGACCG CCGGTCGACCGG 0 0 00 0 0Afl IIICACATGTG CCACATGTGG CCCACATGTGGG0 >90 >900 >90 >90Asc IGGCGCGCC AGGCGCGCCT TTGGCGCGCCAA>90 >90 >90>90 >90 >90Ava ICCCCGGGG CCCCCGGGGG TCCCCCGGGGGA50 >90 >90>9

2、0 >90 >90BamH ICGGATCCG CGGGATCCCG CGCGGATCCGCG10 >90 >9025 >90 >90Bgl IICAGATCTG GAAGATCTTC GGAAGATCTTCC0 75 250 >90 >90BssH IIGGCGCGCC AGGCGCGCCT TTGGCGCGCCAA0 0 500 0 >90BstE IIGGGT(A/T)ACCC010BstX IAACTGCAGAACCAATGCATTGG AAAACTGCAGCCAATGCATTGGAA CTGCAGAACCAATGCATTGGATG

3、CAT0 25 250 50 >90Cla ICATCGATG GATCGATC CCATCGATGG CCCATCGATGGG0 0 >90 500 0 >90 50EcoR IGGAATTCC CGGAATTCCG CCGGAATTCCGG>90 >90 >90>90 >90 >90Hae IIIGGGGCCCC AGCGGCCGCT TTGCGGCCGCAA>90 >90 >90>90 >90 >90Hind IIICAAGCTTG CCAAGCTTGG CCCAAGCTTGGG0 0 100 0

4、75Kpn IGGGTACCC GGGGTACCCC CGGGGTACCCCG0 >90 >900 >90 >90Mlu IGACGCGTC CGACGCGTCG0 250 50Nco ICCCATGGG CATGCCATGGCATG0 500 75Nde ICCATATGG CCCATATGGG CGCCATATGGCG GGGTTTCATATGAAACCC GGAATTCCATATGGAATTCC GGGAATTCCATATGGAATTCCC0 0 0 0 75 750 0 0 0 >90 >90Nhe IGGCTAGCC CGGCTAGCCG CTAG

5、CTAGCTAG0 10 100 25 50Not ITTGCGGCCGCAA ATTTGCGGCCGCTTTA AAATATGCGGCCGCTATAAA ATAAGAATGCGGCCGCTAAACTAT AAGGAAAAAAGCGGCCGCAAAAGGAAAA0 10 10 25 250 10 10 90 >90Nsi ITGCATGCATGCA CCAATGCATTGGTTCTGCAGTT10 >90>90 >90Pac ITTAATTAA GTTAATTAAC CCTTAATTAAGG0 0 00 25 >90Pme IGTTTAAAC GGTTTAAACC

6、 GGGTTTAAACCC AGCTTTGTTTAAACGGCGCGCCGG0 0 0 750 25 50 >90Pst IGCTGCAGC TGCACTGCAGTGCA AACTGCAGAACCAATGCATTGG AAAACTGCAGCCAATGCATTGGAA CTGCAGAACCAATGCATTGGATGCAT0 10 >90 >90 00 10 >90 >90 0Pvu ICCGATCGG ATCGATCGAT TCGCGATCGCGA0 10 00 25 10Sac ICGAGCTCG1010Sac IIGCCGCGGC TCCCCGCGGGGA0 5

7、00 >90Sal IGTCGACGTCAAAAGGCCATAGCGGCCGC GCGTCGACGTCTTGGCCATAGCGGCCGCGG ACGCGTCGACGTCGGCCATAGCGGCCGCGGAA0 10 100 50 75Sca IGAGTACTC AAAAGTACTTTT10 7525 75Sma ICCCGGG CCCCGGGG CCCCCGGGGG TCCCCCGGGGGA0 0 10 >9010 10 50 >90Spe IGACTAGTC GGACTAGTCC CGGACTAGTCCG CTAGACTAGTCTAG10 10 0 0>90 >

8、90 50 50Sph IGGCATGCC CATGCATGCATG ACATGCATGCATGT0 0 100 25 50Stu IAAGGCCTT GAAGGCCTTC AAAAGGCCTTTT>90 >90 >90>90 >90 >90Xba ICTCTAGAG GCTCTAGAGC TGCTCTAGAGCA CTAGTCTAGACTAG0 >90 75 750 >90 >90 >90Xho ICCTCGAGG CCCTCGAGGG CCGCTCGAGCGG0 10 100 25 75Xma ICCCCGGGG CCCCCGGGGG CCCCCCGGGGGG TCCCCCCGGGGGGA0 25 50 >900 75 >90 >90注释：1如果要加