canco软件使用实例CCA分析

1、Canoco软件操作的简单教程步骤：1 .翻开excel文档,如下列图所示,包含环境变量12个和生物因子15种C源利用性.共是8个处理,每个处理三个重复,总共是 24个土壤样品.Potential:potentially mineralizableorganic C pools; SOC, solubleorganic C extracted by 2 M KCl ; SON, soluble organic N extracted by 2MKCl; soil moisture content; TC, Total C; TN, Total N; WHCwater holdingcapaci

2、ty; MBC, microbial biomass C; MBN, Microbial biomass N.The 15 C sources were chosen to cover a range of low molecular weight compounds including carbohydrates, amino acids and carboxylic acids that are commonly in root exudates Campbell et al., 2022. The numbers from 1 to 16 correspond to represent

3、L-alanine, L-arginine, citric acid, D-galactose, amino but yric acid, malic acid, oxalic acid, 3,4-OH benzoic acid,L-arabinose, L-cysteine, D-fructose, D-glucose, L-lysine, N-acetyl glucosamine, D-trehalose, water, respectively.选中区域包括前面的数字,点击复制,然后翻开canoco软件中WcanoIMP如下列图所示.然后选中 save in condensed form

4、at ,然后点击 save.分别保存文件为 environ.dta dta 是canoco默认的样式和species.dta. 文 件形式如下列图.2 .翻开canoco软件一new project ,得到下面的界面.3 .点下一步后,有三个 brown窗口,前 2个分别选择 environ.dta 和species.dta.第三个选择软件自带适宜的处理方法如CC掰口 RDA等.本文是选用RDg析.4 .其他还有一些窗口,但是问题不大,这里就不多说了.直接进入下一步,点击 analyze ,如下列图所示.完成后点击canodraw.5 .点击create窗口,如下列图所示.得到最后的结果.结果

5、的解释：The relationships between environmental factors and microbial metabolic profiles were as shown above. Plots can be interpreted quantitatively using the length of the arrow to indicate how much variance was explained by that factor;thedirection ofthearrows for individualenvironmentalfactorindicat

6、ing an increasing weighting of that factor. The soil sample arrows pointinginapproximatelythesame direction as theenvironmentalarrowsindicate a high positive correlation (the longer the soil sample arrow, the stronger the relationship; Kennedy et al., 2022). Among all environmental factors, C 0 exhi

7、bited the greatest influence on the soil samples (P0.01) followed by pH and soil moisture and SON content.What is driving changes?Previous studies have suggested that RDAwas a suitable method for quantify the relationshipbetween environmental factors and changes in microbialcommunity of soils (Kenne

8、dy et al., 2022; Macdonald et al., 2022). In this study, we found that classified microbial metabolic profiles based on cover crop treatments and quantified the contributions of each environmental factor to soil samples, aiming to which environmental factor majorly influenced the changes of microbia

9、l metabolic profiles between the CKand cover crop treatments. Recent studies have shown the soil microbial metabolic profiles is strongly affected by abiotic environmental factors, such as Soil total C (Degens et al., 2000), SOC (Huang et al., 2022) and C:N ratio (Graham and Haynes, 2022). However,

10、we found that C 0, rather than other environmental factors, was more indicative of the change of microbial metabolic profiles.相关的文献：Degens, B.P., Schipper, L.A., Sparling, G.P., Vojvodic-Vukovic, M. 2000.Decreases in organic C reserves in soils can reduce the catabolic diversity of soil microbial co

11、mmunities. Soil Biol. Biochem. 32, 189-196.Graham, M.H., Haynes, R.J. 2022. Catabolic diversity of soil microbial communities under sugarcane and other land uses estimated by Biolog and substrate-induced respiration methods. Appl. Soil Ecol. 29, 155-164.Huang, Z.Q., Xu, Z.H., Chen, C.R. 2022. Effect

12、 of mulching on labile soil organic matter pools, microbial community functional diversity and nitrogen transformations in two hardwood plantations of subtropicalAustralia. Appl.Soil Ecol. 40, 229-239.Sissoko, A., Kpomblekou-A, K. 2022. Carbon decomposition in broilerKennedy, N., Brodie, E., Connolly, J., Clipson, N. 2022. Impact of lime, nitrogen and plant species on bacterial community structure in grassland microcosms. Environ. Microbiol. 6, 1070-1080.Macdonald, C.A., Thomas, N., Robinson, L., Tate, K.R., Ross, D.J.