Hoagland-营养液配方(试验总结)(共3页)

1、精选优质文档-倾情为你奉上Hoagland 营养液配方， 荷格伦特营养液的配方如下: 改良霍格兰配方： 四水硝酸钙 945mg/L 硝酸钾 506mg/L 硝酸铵 80mg/L 磷酸二氢钾 136mg/L 硫酸镁 493mg/L 铁盐溶液 2.5ml 微量元素液 5ml pH=6.0 铁盐溶液：七水硫酸亚铁 2.78g 乙二胺四乙酸二钠（EDTA.Na） 3.73g 蒸馏水 500ml pH=5.5 微量元素液：碘化钾 0.83mg/l 硼 酸 6.2mg/L 硫酸锰 22.3mg/L 硫酸锌 8.6mg/L 钼酸钠 0.25mg/L 硫酸铜 0.025mg/L 氯化钴 0.025mg/L 若

2、作为复合肥使用，可以采用天然水配制，省略微量元素液。若作为无土栽培营养液需用人工软水配制，如蒸馏水，微量元素液必须加入。 经常将上述营养液配成10倍或20倍浓度，用时稀释即可。注意用前调整pH。我打算先配2L，大量元素：需Ca(NO3)2·4H2O：1.89g，KNO3：1.012g，NH4 NO3:0.16g，KH2PO4：0.272g，MgSO4·7H2O：0.986g。微量元素：碘化钾 1.66g ，硼 酸 12.4g ，硫酸锰：44.6g， 硫酸锌 17.2g 钼酸钠 0.5g 硫酸铜 0.05g 氯化钴0.05g。铁盐：七水硫酸亚铁：2.78g，乙二胺四乙酸二钠（

3、EDTA.Na）3.73g。整个配制大约需要：5L蒸馏水。调PH的盐酸，pH试纸。500mL容量瓶一个，1L的容量瓶3个，玻棒和烧杯若干。1.大量元素 每升培养液中加入克数或摩尔数盐类浓度g/Lmol/Lg/2Lg/15 LCa(NO3)2·4H2O1.180.0052.3617.7KNO30.510.0051.027.65MgSO4·7H2O0.490.0020.987.35KH2PO40.140.0010.282.12.微量元素 每升培养液中加入的毫克数盐类浓度（mg/L）浓度（mg/2L）mg/15 LH3BO32.865.7242.9MnCl2·4H2O1

4、.813.6227.15ZnSO4·7H2O0.220.443.3CuSO4·5H2O0.080.161.2H2MoO4·H2O/Na2MoO4·2H2O0.02/0.030.040.48先配g/L的微量元素溶液，然后吸取1ml+大量元素稀释1000倍定容。3.每升培养液中加入2 mL FeEDTA溶液(即乙二胺四乙酸铁盐溶液)。铁盐溶液：七水硫酸亚铁 2.78g 乙二胺四乙酸二钠（EDTA-Na2）3.73g 蒸馏水 500 mL 配制500 mL的铁盐溶液：1. 取两只洁净干燥烧杯，分别加入3.73 g EDTA-Na2和2.78 g FeSO4&#

5、183;7H2O固体。2. 取1 L纯水煮沸，冷却至70后，量取300 mL，加入装有EDTA-Na2的烧杯中，进行搅拌溶解。3. 量取200 mL纯水，冷却后加入另一烧杯中，搅拌溶解。4. 然后将FeSO4·7H2O溶液缓缓加入到EDTA-Na2溶液中，边加边搅拌。5. 最后，将铁盐溶液倒入棕色瓶中（黑暗），低温保存。通常应当先配出各种盐类的浓缩液。注意避免浓缩液中出现沉淀。使用时按一定的比例加水稀释到要求的浓度。采用循环供液时，营养液中的矿质元素被植物体吸收后，应当及时进行调节，使营养液仍旧符合原配方的要求。 以下 是老外的 有些看不懂 呵呵Recipe for Hoagland

6、's Complete Nutrient Solution This is made essentially according to the following reference: D.R. Hoagland and D.I. Arnon. The water-culture method of growing plants without soil. Calif. Agr. Expt. Sta. Circ. 347. 1950. There is one change and that is in the form of iron added. Prepare the follo

7、wing stock solutions (1-6) and use the amounts indicated to prepare 1 liter ( final volume ) of nutrient solution: 1. 1.00 M NH4H2PO4 use 1 mL/L of nutrient solution 2. 1.00 M KNO3 use 6 mL/L of nutrient solution 3. 1.00 M Ca(NO3 )2 use 4 mL/L of nutrient solution 4. 1.00 M MgSO4 use 2 mL/L of nutri

8、ent solution Micronutrient stocks: combine the following amount of salts in a total volume of one liter of water, and then use 1 mL/L of this entire stock mixture (5) along with the stocks above (1-4) and the iron stock (6) described below to make up a total of 1 L of nutrient solution. 5. 2.86 gm H

9、3BO3 1.81 gm MnCl2 .4H2O 0.22 gm ZnSO4 .7H2 O 0.08 gm CuSO4 . 5H2O 0.02 gm H2MoO4 . H2O (Assaying 85% MoO3) 6. Iron stock: to the above 5 stocks add 0.25 ml of this iron stock for 1 liter of nutrient solution. To make up the iron stock, take 26.1 g EDTA and dissolve in 286 ml water that has 19 g KOH

10、 . Then dissolve 24.9 FeSO4.7H2O in 500 ml water. Slowly add the iron sulfate solution to the potassium EDTA solution and aerate this solution overnight with stirring. The pH rises to about 7.1 and the solution is wine red and very little precipitation occurs. Make to 1 liter final volume and store in a bottle covered with