10份贵州常用玉米自交系丝黑穗病抗性鉴定及遗传多样性分析

1、10份贵州常用玉米自交系丝黑穗病抗性鉴定及遗传多样性分析p 打开文本图片集【摘要】：p ：【目的】对贵州常用玉米自交系进行丝黑穗病抗性鉴定、主要农艺性状评价及SSR遗传多样性分析p ，为贵州玉米的抗丝黑穗病基因挖掘及优质抗性种质资创建提供参考依据。【方法】以10份贵州常用玉米自交系材料为研究对象，于20_20_年分别在贵州安顺市平坝实验基地及贵阳市花溪区贵州大学鱼塘教学试验场进行丝黑穗病抗性鉴定;采用136对条带清晰、重复性好的SSR分子标记对供试材料进行PCR扩增检测，并运用POPGENE 32和NTSYS-2.10e进行遗传多样性和聚类分析p 。【结果】连续两年两地的玉米丝黑穗病抗性鉴定结

2、果表明，自交系T32和齐319表现为稳定高抗，感病率为0;自交系J51和J106表现为高感，平均感病率分别为64.18和48.62，其余6个自交系表现为感病。10份贵州常用玉米自交系材料在贵州地区均能正常成熟，其中以T32的综合表现较突出，为贵州优势种质材料，J51、J106和双M9次之，齐319的表现相对较差。136对SSR分子标记从10份贵州常用玉米自交系材料中共检测到567个等位基因，平均为4.17个;Neis基因多样性指数（H）为0.1800.500，平均为0.328;多态性信息量（PIC）为0.1500.920，平均为0.656;多态性最好的SSR分子标记为umc1795。以相似系数

3、0.658为界，10份贵州常用玉米自交系可划分为三大类：类群包括苏11、T32、S37和双M9，均具有熱带血缘;类群包括齐319、J106、HC、郑58和QL16，均具有温带血缘;类群为贵州地方种质J51。【结论】贵州常用玉米自交系材料具有较广泛的遗传基础，可满足玉米育种对亲本遗传差异的基本要求。尤其是自交系T32高抗玉米丝黑穗病且在贵州山区具有良好的适应性，为热带血缘Suwan选系，明显区别于改良Reid选系、先锋选系、美国杂交种选系及贵州地方种质，可优先用于贵州地区抗丝黑穗病基因发掘、杂交种改良及优良抗性种质资创建。【关键词】：p ： 玉米;自交系;丝黑穗病;SSR分子标记;抗性鉴定;贵州

4、中图分类号： S513.024 文献标志码： A 文章编号：2095-1191（20_）11-2384-08Identification of head smut resistance and analysis of geic diversity of 10 monly used maize inbred lines in GuizhouTAN Kang， LI Chun-hong， YANG Mei， SHEN Tao， QIU Hong-bo_（College of Agriculture， Guizhou University，Guiyang 550025， China）Abstract

5、：【Objective】To analyze the identification of head smut resistance， evaluation of main agronomic traits and analysis of SSR geic diversity of mon maize inbred lines in Guizhou， and to provide a reference basis for head smut resistance gene mining and breeding of high-quality resistance germplasm reso

6、urces of maize in Guizhou.【Method】Taking 10 copies of Guizhou mon maize inbred lines as research objects， the resistance identification test for head smut was performed at the Pingba E_perimental Base in Anshun， Guizhou， and the fish pond teaching e_perimental field of Guizhou University， Hua_i Dist

7、rict， Guiyang from 20_ to 20_.A total of 136 pairs of SSR molecular markers with clear and reproducible bands were used for PCR lification of test materials.Geic diversity and cluster analysis were performed by POPGENE 32 and NTSYS-2.10e.【Result】The identification of head smut resistance in two plac

8、es for two consecutive years showed that inbred lines T32 and Qi 319 showed stable high resistance with a susceptibility rate of 0; inbred lines J51 and J106 showed high susceptibility with susceptibility rates of 64.18 and 48.62， and the other si_ inbred lines showed susceptibility.The analysis of

9、main agronomic traits showed that all of the tested materials could grow in Guizhou mountainous area， among which inbred line T32 was the best， followed by J51， J106 and Shuang M9， and Qi 319 was the worst.Geic diversity analysis showed that 567 alleles were detected in 10 maize materials with 136 p

10、airs of SSR markers， averaging 4.17 alleles， Neis gene diversity inde_（H） was 0.328 on average， ranging from 0.180 to 0.500.Polymorphism information quantity （PIC） was 0.656 on average， ranging from 0.150 to 0.920.The umc1795 polymorphism was the best SSR marker.Taking the similarity coefficient of

11、0.658 as the boundary， 10 copies of Guizhou mon maize inbred lines could be divided into three major groups：Group I included Su 11， T32， S37 and Shuang M9， all of which had tropical geics;group II included Qi 319， J106， HC， Zheng 58 and QL16，they had temperate zone gene-tics; and group III was Guizh

12、ou local germplasm J51.【Conclusion】The mon maize inbred line materials in Guizhou have a broad geic basis， which can meet the basic requirements for geic differences of parents in maize breeding;The inbred line T32 is highly resistant to maize head smut and has good adaptability in the mountainous areas of Guizhou.It is a tropical Suwan line， which is different from the improved Reid line， Pioneer line， American hybrid line and Guizhou loca