关于氨基酸和神经肽与疼痛关系的研究

1、 std aa氨基酸分离标准图氨基酸分离标准图 csf sample (n = 30)(n = 38) labor pain group(n = 38)pi vs. arginine精氨酸精氨酸 correlation coefficient 相关系数相关系数p value p值值0.0350.706pi vs. asparagine天冬酰胺酸天冬酰胺酸 correlation coefficientp value-0.207 *0.026pi vs. aspartate天天(门门)冬氨酸盐冬氨酸盐 correlation coefficientp value0.285 *0.002pi vs

2、. citrulline瓜氨酸瓜氨酸 correlation coefficientp value0.501 *0.001pi vs. gaba伽马氨基丁酸伽马氨基丁酸 correlation coefficientp value0.0190.835pi vs. glutamate谷氨酸谷氨酸 correlation coefficientp value0.0600.518pi vs. glutaminecorrelation coefficientp value-0.601 *0.001pi vs. glycinecorrelation coefficientp value0.332 *0.

3、001pi vs. taurinecorrelation coefficientp value-0.307 *0.001pi pain intensity graded as mild轻度 (n = 11), moderate 中度(n = 18), severe严重 (n = 9) * (p 0.05) * (p 0.01) * (p 0.001) statistically significant correlation relative to the control group.amino acidsage (mean sd)control groupa (mol/l)(n = 30)3

4、3.4 5.0labor pain groupb(mol/l)(n = 38)28.6 4.5alanine丙胺酸丙胺酸 29.41 1.3333.41 1.53cystine胱氨酸胱氨酸 0.6 0.12 0.2 0.06*histidine组氨酸组氨酸 14.87 1.1317.39 0.84isoleucine异亮氨酸异亮氨酸 3.77 0.154.03 0.19leucine亮氨酸亮氨酸 8.56 0.379.09 0.35lysine赖氨酸赖氨酸 18.72 1.3819.34 1.31methionine蛋氨酸蛋氨酸 1.85 0.132.19 0.10*phenylalanine

5、苯基丙氨酸苯基丙氨酸 7.26 0.297.94 0.27proline脯氨酸脯氨酸 0.89 0.091.22 0.09*serine丝氨酸丝氨酸 32.05 1.2724.55 0.87*threonine苏氨酸58.68 2.7359.94 3.16tryptophan色氨酸1.87 0.102.26 0.10*tyrosine酪氨酸3.94 0.434.95 0.44valine缬氨酸9.59 0.5010.02 0.38 脑脊液中生理氨基酸和疼痛的联系 amino acids氨基酸氨基酸age (mean sd)年龄（平均数年龄（平均数 方差）方差）controla (mol/l)(

6、n = 35)37.6 12.2标准组chronic painb (mol/l)(n = 29)64.7 12慢性疼痛组acute painc (mol/l)(n = 46)36.8 19.1急性疼痛组*results chronic pain vs no pain group aspartate, glutamate, glycine and no involved in chronic pain perception 天门冬氨酸盐，谷氨酸，甘氨酸和一氧化氮与疼痛的感知有关 chronic pain vs acute pain differential roles of amino acid

7、s in chronic and acute pain states氨基酸在慢性和急性疼痛状态的不同作用 aspartate - a key player in pathogenesis of chronic pain 天门冬氨酸盐导致慢性疼痛的重要因素 identificationcharacterisationquantificationlocalisationcns effectsantagonistspain models mkilfcdllllslfssvssscqkdclvcreklrptldsfslemcileceekaftsplwtpbovine60mkvllcdllllslf

8、ssvfsscqrdcltcqeklhpaldsfdlevcileceekvfpsplwtphuman60mkilfcdvlllsllssvfsscprdcltcqeklhpapdsfnlktcilqceekvfprplwtvmouse60mkilfcdvlllsllssvfsscpedcltcqerlhpapgsfnlklcilqceekvfprplwtlrat60signal peptidectkvmargswqlspadpdhvaaaldqprasemqhibovine108ctkvmarsswqlspaapehvaaalyqprasemqhihuman108ctkvmasgsgqlsp

9、adpelvsaalyqpkasemqhimouse119ctkamasdseqlspadpeltsaalyqskasemqhirat113krmprvrslfqrq-bovine121rrmprvrslfqeq-human121krmprvrslvqvrdaepgadaepgmouse132krmprvrsvvqardaepea-rat127bpnp-2krtepgleevgeieqkqlqkrfggftgarksarklanqbovine159eepepgmeeagemeqkqlqkrfggftgarksarklanqhuman159adaepgaddaeeveqkqlqkrfggftga

10、rksarklanqmouse170-daepvadeadeveqkqlqkrfggftgarksarklanqrat164bpnp-3 (nocistatin)nociceptinkrfsefmrqylvlsmqssqrrrbovinekrfsefmrqylvlsmqssqrrrhumankrfsefmrqylvlsmqssqrrrmousekrfsefmrqylvlsmqssqrrrratbpnp-4tlhqngna176tlhqngnv176tlhqngnv187tlhqngnv181bpnp-5immunofluorescent confocal images of sections

11、of mouse brain incubated with anti-nocistatin antiserum. a transverse section of mouse brain was incubated with anti-mpnp-3 antibody followed by cy3-conjugated anti-rabbit igg. bar, 250 m (a, b, c, d); 100 m (c, d). human nocistatin and nociceptinrrmprvrslfqeq-eepepgmeeagemeqkqlqkrfggftgarksarklanqkrhuman148krmprvrslvqvrdaepgadaepgadaepgaddaeeveqkqlqkrfggftgarksarklanqkrmouse159krmprvrsvvqardaepea-daepvadeadeveqkqlqkrfggftgarksarklanqkrrat153krmprvrslfqrq-krtepgleevgeieqkqlqkrfggftgarksarklanqkrbovine148nociceptin96969696nocistatinnocistatin okuda-ashitaka e, tachibana s et al, noci