紫外分析方法

1、 %11cmE%11cmEmin%11maxmax，shcmE%11cmE定性鉴别：药典规定维生素12B三处最大吸收，55036127888. 170. 1278361AA45. 315. 3550361AA%06. 01002101 . 1%/101 . 1100/101 . 1143505. 0334%11肾上腺酮mlmgmlglEACcm%11cmElECA定量依据： 下测定下测定须在须在较小的较小的左右左右测定灵敏度高测定灵敏度高maxmaxmaxmaxlCAEAA样样参参调调节节光光路路光光学学性性质质和和厚厚度度相相同同样样品品池池样样品品溶溶液液，参参比比池池空空白白溶溶液液样样

2、品品池池参参比比池池配配制制样样品品的的溶溶剂剂空空白白溶溶液液ATA%10001吸光系数法（绝对法）：吸光系数法（绝对法）：前提：要求是平行单色光前提：要求是平行单色光iECACigW求含样过程：已知或样稀释/)(lEAmlgCi100/lALmolCi/或%100%样CCiiA=EClmlgmlglEAC/0 .20)100/(00200. 01207414. 0mLgmLgCi/1045.2100/1045.21207507.053mLgC/51050.21001010021050.2样%0 .98%10051050.251045.2%100%12样CiCB%)0 .764(591. 0

3、1001025000.20367255iEAmLmLmLmgmlmLHCl求求已已知知测测移移取取稀稀至至样样品品稀稀至至吡吡啶啶 mlgmlglEAiC/61092. 7100/41092. 7764591. 0%0 .99%10010010250100 . 21092. 7%100%26样样CCii解： 9 .927%11cmEmlgmlgiC/610900. 4100/410900. 419 .927463. 0)/(10000. 55052001000.1063mlgC样样%0 .98%10010000. 510900. 4%100%66样样咖咖啡啡酸酸CCi5052001稀释因子稀释

4、因子注：注：%11cmE12000 .100120001010%11MEcmmlgmlglETlEAiC/610165. 3100/410166. 311200417. 0lglgmlgC/610000. 410022500500. 0样%15.79%100610000. 4610165. 3%100%样样品CiCABBA离解离解KxBA令令x010 x000602.则则207110010001020414. 03%11ClAEcm%.%6961001020101207400010010201001000lEA3311cm原料药原料药)/(.%mlmg250000110012075180001

5、100100010lEA11cm注射液注射液条件前提：固定仪器和测定CACKA样样同上条件固定条件查得测定样品曲线分别测定配制标准系列过程：CAACAbxaynxbyaniniii11 niniiinininiiiiixxnyxyxnb1122111)(niniiiniiiyyxxyyxx11221)()()() 1|0(mlmgmlmlmlmg250 . 32550/200. 0样品标准样品分别移取标样与样品浓度相近固定仪器和测定条件；前提：siAA和和分别测定分别测定一定一定过程：过程：SiSiAACCsCiCCSiSiAAmm%100%mmiilECAiilECAssmlgCAACCis

6、isi/1 .985 . 2101000518. 0508. 0100000.25%1 .98100%12标示量标示量iCB5 . 210010101207508. 06iiClEACmLgiC/1 .98%1 .98100%12标示量标示量iCBHKKAHAHKCKHKHCAHAAaaAHAaaAaAHAHAHAAAAHAAaK混混AApHpKa-InAHInAlg整理得：整理得：A(max=590nm)pH40.430HIn与In-碱1.024In-酸0.002HIn混混AInAHInAApHapKlg14. 4430. 0024. 1002. 0430. 0lg4apK 混混AInAHInAApHapKlg736. 7314. 0064. 0773. 0314. 0lg8apK736. 7332. 0064. 0773. 0332. 0lg pHapK 混混AAAApHpKAHAalg混混AAAHAAApHapKlg00. 2HAA0AAA混4 =0.50pH混4=5.40A混1 =1.78pH