量子点在生物分析中的应用

1、量子点在生物分析中的应用 2010.12.08量子点在生物及医学方面的应用量子点在DNA杂交分析中的应用 Scheme 1：Schematic Representation of GO-Induced Fluorescence Quenching of MB-QDs and Biosensing Mechanism Anal. Chem. 2010, 82, 55115517 Figure 1 ： Fluorescence emission spectra of (A) QDs (120 nM), (B)MB-QDs (120 nM), and (C) MB-QDs incubated wit

2、h target (800nM) in HB buffer before (a) and after (b) adding GO (0.1 g/mL) for5 min.量子点在特定生物识别中的应用Scheme 2：QD-encoded aptamer-linked nanostructures for multiplex detection. Anal. Chem. 2007, 79, 4120-4125 Figure 2. Steady-state emission spectra of mixed sensors. (A) Mixed sensor alone, or with 1 mM

3、 cytidine, or with 1 mM cytidine and 1 mM uridine. (B) Mixed sensor with 1 mM adenosine added. (C) Mixed sensor with 1 mM cocaine added. (D) Mixed sensor with 1 mM adenosine and 1 mM cocaine added.量子点在检测细胞表面糖蛋白的应用 Schematic Representation of the Monolayer Fabrication and the Competitive Assay J. AM.

4、 CHEM. SOC. 2008, 130, 72247225 Figure 3. Topographic images of (A) MUA/Au, (B) ADH/MUA/Au, (C)mannan/ADH/MUA/Au, and (D) QD-Con A/BSA-blocked mannan/ADH/MUA/Au. 量子点在检测细胞内代谢途径中的应用 Figure 4. a) Sensing of NADH by Nile-blue-functionalized CdSe/ZnS QDs. b) Timedependent fluorescence changes as a result

5、 of the interaction of the functionalized QDs with 0.5 mm NADH.c) Fluorescence intensity ratios of the modified QDs after the addition of different concentrations of NADH and the fluorescence intensities prior to the addition of NADH. Angew. Chem. Int. Ed. 2009, 48, 309 313 Figure 5. a) Sensing of e

6、thanol by Nile-blue-functionalized CdSe/ZnS QDs. b) Timedependent fluorescence changes for the analysis of 1 mm ethanol by the functionalized QDs. c) Calibration curve corresponding to the optical analysis of different concentrations of ethanol by the functionalized QDs. Each sample was analyzed aft

7、er reaction of the functionalized QDs for 18 min. Figure 6. a) Confocal fluorescence microscope image of a HeLa cell that included incorporated Nile-blue-functionalized QDs. b) (1) Time-dependent fluorescence changes of HeLa cells that include the functionalized QDs upon interaction with 50 mm d-glu

8、cose. (2) Time-dependent fluorescence changes of HeLa cells that include the functionalized QDs upon interaction with 50 mm l-glucose. Each data point corresponds to the analysis of 20 different cells. c) Time-dependent fluorescence changes of HeLa cells that include functionalized QDs upon addition

9、 of 50 mm d-glucose to (1) nontreated HeLa cells, and (2) taxoltreated HeLa cells. 量子点在生物医学应用中存在的主要问题 由于部分量子点含有重金属毒性离子, 而这些金属离子对肺和肾脏都具有毒性。所以随着量子点在生物医学研究中的广泛应用, 与此密切相关的药物毒性问题, 以及对人类环境可能产生的污染问题都日渐引起研究者的高度关注。由于目前的研究主要集中在对量子点的实验和应用的可行性验证层次, 而量子点在生物微环境下的稳定性, 细胞内外的分布, 以及聚集现象等都与其自身和细胞的化学组分, 形态和大小密切相关。因此, 还有不少的问题尚待解决, 许多的应用技术有待于开拓和发展。 小结 总的来说，由于量子点技术有其独特的标记特点，它必将成为今后生物分子检测的尖端技术，为DNA检测（DNA芯片）、蛋白质检测（蛋白质芯片）和探索蛋白质-蛋白质之间（抗原-抗体、配体-受体、酶-底物）反应原理提供更先进的方法。同时也将极大地推进生物显像技术和生物制药技