关于氨基酸和神经肽与疼痛关系的研究参考PPT

1、12345678910 std aa氨基酸分离标准图氨基酸分离标准图11 csf sample12 1314(n = 30)(n = 38) 15labor pain group(n = 38)pi vs. arginine精氨酸精氨酸 correlation coefficient 相关系数相关系数p value p值值0.0350.706pi vs. asparagine天冬酰胺酸天冬酰胺酸 correlation coefficientp value-0.207 *0.026pi vs. aspartate天天(门门)冬氨酸盐冬氨酸盐 correlation coefficientp v

2、alue0.285 *0.002pi vs. citrulline瓜氨酸瓜氨酸 correlation coefficientp value0.501 *0.001pi vs. gaba伽马氨基丁酸伽马氨基丁酸 correlation coefficientp value0.0190.835pi vs. glutamate谷氨酸谷氨酸 correlation coefficientp value0.0600.518pi vs. glutaminecorrelation coefficientp value-0.601 *0.001pi vs. glycinecorrelation coeffi

3、cientp value0.332 *0.001pi vs. taurinecorrelation coefficientp value-0.307 *0.001pi pain intensity graded as mild轻度 (n = 11), moderate 中度(n = 18), severe严重 (n = 9) * (p 0.05) * (p 0.01) * (p 0.001) statistically significant correlation relative to the control group.16amino acidsage (mean sd)control

4、groupa (mol/l)(n = 30)33.4 5.0labor pain groupb(mol/l)(n = 38)28.6 4.5alanine丙胺酸丙胺酸 29.41 1.3333.41 1.53cystine胱氨酸胱氨酸 0.6 0.12 0.2 0.06*histidine组氨酸组氨酸 14.87 1.1317.39 0.84isoleucine异亮氨酸异亮氨酸 3.77 0.154.03 0.19leucine亮氨酸亮氨酸 8.56 0.379.09 0.35lysine赖氨酸赖氨酸 18.72 1.3819.34 1.31methionine蛋氨酸蛋氨酸 1.85 0.13

5、2.19 0.10*phenylalanine苯基丙氨酸苯基丙氨酸 7.26 0.297.94 0.27proline脯氨酸脯氨酸 0.89 0.091.22 0.09*serine丝氨酸丝氨酸 32.05 1.2724.55 0.87*threonine苏氨酸58.68 2.7359.94 3.16tryptophan色氨酸1.87 0.102.26 0.10*tyrosine酪氨酸3.94 0.434.95 0.44valine缬氨酸9.59 0.5010.02 0.381718 脑脊液中生理氨基酸和疼痛的联系 19amino acids氨基酸氨基酸age (mean sd)年龄（平均数年

6、龄（平均数 方差）方差）controla (mol/l)(n = 35)37.6 12.2标准组chronic painb (mol/l)(n = 29)64.7 12慢性疼痛组acute painc (mol/l)(n = 46)36.8 19.1急性疼痛组20*21results chronic pain vs no pain group aspartate, glutamate, glycine and no involved in chronic pain perception 天门冬氨酸盐，谷氨酸，甘氨酸和一氧化氮与疼痛的感知有关 chronic pain vs acute pain

7、 differential roles of amino acids in chronic and acute pain states氨基酸在慢性和急性疼痛状态的不同作用 aspartate - a key player in pathogenesis of chronic pain 天门冬氨酸盐导致慢性疼痛的重要因素 222324 25identificationcharacterisationquantificationlocalisationcns effectsantagonistspain models2627 2829303132 3334353637mkilfcdllllslfs

8、svssscqkdclvcreklrptldsfslemcileceekaftsplwtpbovine60mkvllcdllllslfssvfsscqrdcltcqeklhpaldsfdlevcileceekvfpsplwtphuman60mkilfcdvlllsllssvfsscprdcltcqeklhpapdsfnlktcilqceekvfprplwtvmouse60mkilfcdvlllsllssvfsscpedcltcqerlhpapgsfnlklcilqceekvfprplwtlrat60signal peptidectkvmargswqlspadpdhvaaaldqprasemqh

9、ibovine108ctkvmarsswqlspaapehvaaalyqprasemqhihuman108ctkvmasgsgqlspadpelvsaalyqpkasemqhimouse119ctkamasdseqlspadpeltsaalyqskasemqhirat113krmprvrslfqrq-bovine121rrmprvrslfqeq-human121krmprvrslvqvrdaepgadaepgmouse132krmprvrsvvqardaepea-rat127bpnp-2krtepgleevgeieqkqlqkrfggftgarksarklanqbovine159eepepgm

10、eeagemeqkqlqkrfggftgarksarklanqhuman159adaepgaddaeeveqkqlqkrfggftgarksarklanqmouse170-daepvadeadeveqkqlqkrfggftgarksarklanqrat164bpnp-3 (nocistatin)nociceptinkrfsefmrqylvlsmqssqrrrbovinekrfsefmrqylvlsmqssqrrrhumankrfsefmrqylvlsmqssqrrrmousekrfsefmrqylvlsmqssqrrrratbpnp-4tlhqngna176tlhqngnv176tlhqngn

11、v187tlhqngnv181bpnp-5383940414243444546immunofluorescent confocal images of sections of mouse brain incubated with anti-nocistatin antiserum. a transverse section of mouse brain was incubated with anti-mpnp-3 antibody followed by cy3-conjugated anti-rabbit igg. bar, 250 m (a, b, c, d); 100 m (c, d).

12、 474849505152human nocistatin and nociceptinrrmprvrslfqeq-eepepgmeeagemeqkqlqkrfggftgarksarklanqkrhuman148krmprvrslvqvrdaepgadaepgadaepgaddaeeveqkqlqkrfggftgarksarklanqkrmouse159krmprvrsvvqardaepea-daepvadeadeveqkqlqkrfggftgarksarklanqkrrat153krmprvrslfqrq-krtepgleevgeieqkqlqkrfggftgarksarklanqkrbovine148nociceptin96969696nocistatinnocistatin535455 5657okuda-ashitaka e, tachibana s et al, nocistat