如何测定荧光量子效率

1、如何测定荧光量子效率首先要有一个已知量子产率的标准物质，一般用硫酸奎宁。分别做标准物质和未知 样品的吸收和荧光光谱。 u=O s(Yu/Ys)(As/Au)u -待求物质的荧光量子产率；s -准物质的荧光量子产率；Yu 待求物质的荧光积分面积；Ys 标准物质的荧光积分面积；Au 待求物质的荧光激发波长处的吸收值；As 标准物质的荧光激发波长处的吸收值。测定激发光强度和荧光强度就可以了知道吧？如果已知一标准物质的量子产率，可以配置相同浓度的标准和待测物质，在相同的 测量条件下，测量两者的荧光强度未知物质的荧光量子产率=F未知/F标准*标准 的量子产率。量子效率是指发射的光子数 Nf与激发时吸收的

2、光子数NX之比，即量子效率=Nf/NX我们知道，一般的发光总有一定的能量损失，激发光子的能量总是大于发射光子的 能量.所以，量子效率通常都是小于100%.在实际测量时我们不好去测量发射的光子 数Nf与激发时吸收的光子数NX,而通常用用下面的公式求算：量子效率=发射峰的最大发光强度/激发峰的最大发光强度但是注意根据待测物的激发和发射波长的范围，选择不同激发和发射波长的基准 物。请问在有机电致发光中关于量子效率中的外量子效率是如何计算的公式是什么样子的exp(eV/kT)=aP2+bP+c(1)其中V为光生电压，k为玻尔兹曼常数，T为绝对温度，e为电子电荷，a，b，c为 常数。1.Additio

3、nally,-NPN thin film emits bright bluefluoresce nee (461 nm)with a quantum efficiency of 8.5%,higher than that of -NPD(5.5%). I 该化合物可发明亮的蓝色 荧光(461 nm),积分球测定其薄膜荧光量子效率为8.5%, 比a-NPD的5.5%高。收藏指正2.Their fluoresce nee emissi on properties were studied and their photolysis kin etics was in vestigated in dif

4、fere nt media by means of steady state photolysis method.考察了其荧光光谱特性，发现甲氧基苯甲酰亚甲基光产碱剂的荧光量子效率较低，而萘甲酰亚甲基光产碱剂较高。收藏指正3.The fluoresce nee qua ntum efficie ncy of oxaz ine 1 perohlorate in 1,2- dichloro-etha ne was measured with photoacoustic speetroscopy in which the fluoresce nee que nchi ng wag used.利用光声

5、光谱技术，采取猝灭的方法对(口恶)嗪1高氯酸盐的二氯乙烷溶液进行 荧光 量子效率的测量。收藏指正4.The experimental results indicate that the quantum efficiency for the emission at 1.5 卩 m is higher upon excitation at 0.98 卩 m than that at 0.80 owi ng to the much weaker excited state absorpti on (ESA) compared withthose of excitation at 0.80卩 m.研究

6、表明：由于在0 .98卩谥激发下，激发态吸收较0 .80卩谥激发下小得多,因而其 1.5荧光发射量子效率也比0 .80 yn激发下高得多；收藏指正5.Fluoresce nee property of coumari n derivative CND_(500) with节cyclodextri n ( -CD) in aqueous solution and CND_(500) with ethyl alcohol,n-saf Trit on X-100 micelle soluti on are exam ined byfluoresce nee method. Itis observed

7、 that the fluoresce nee peak, relative inten sity (F), polarizati on (P), relative quantum yield (?)叮心匚用荧光法研究了香豆素衍生物CND_(500)在伕CD水溶液中和在乙醇及非离子表 面活性剂(n-Saf)Triton X-100等胶束溶液中的荧光特性,发现CND_(500)在 伕CD 水溶液中的荧光强度(F),荧光偏振度(P)、相对量子效率(?)收藏指正6.Abstract: Polyphenylacetylenes were synthesized by Rn(nbd)Cl 2 andWC

8、I6/Ph4S n catalyst systems in N2 at room temperature.Polymers withhigh yield and high molecular weight were obtai ned.Their structures were characterized by UV,IR,NMR and GPC,respectively.Thefluoresce nee of thepolymer solutio n with differe nt concen tratio n was in vestigated using variable excita

9、tion wavelength from UV to visible region.The relation betwee n fluoresce neeand polymer structure was studied.The resultsshowed that the molecular cha in structure has great in flue nee on fluoresce nee of the polymers.The emitt ing peak wavele ngth of fluoresce nee for the polymer of high stereore

10、gularity is in depe ndent of solution concentration and excitation wavelength.The emitting peak wavele ngth of fluoresce nee for the polymer with disordered molecular cha in arran geme nt varies with cha nging the solutio n concen tratio n and excitati on wavele ngth,a nd differe nt emissi on sites

11、in molecular cha ins and low emitti ng yield are show n. The measureme nt and an alysis of fluoresce nee spectroscopy of polyphe ny lacetyle nes may provide some useful information to judge the molecular chain arrangement in someI打；eon jugated polymers.文摘:使用有机金属铑、金属钨/四苯基锡催化剂体系聚合苯乙炔，分别获得了高产 率和高分子量的聚苯

12、乙炔.使用UV, IR, NMR , GPC等分别对聚合物结构进行了表征.采用不同波长的激发光对聚合物 荧光性能进行研究，详细分析了聚合物结构 与荧光性能之间的关系研究结果发现，不同聚合物结构对聚合物 荧光性能产生很 大影响，规整性高的聚合物，将有较高的发光量子效率；聚合物规整性差，将可能导致多个荧光发光结构点，其荧光强度降低.聚合物荧光光谱研究将对某些共轭聚 合物结构的规整性分析提供一些有用的信息收藏指正7.The rate constant and theefficiency of main energy trasfer process werecalculated fromfluores

13、ce neelifetime, phosphoresce nee lifetime,qua ntumyield and time-resolved spectra of I3MK and I3MK-Tb(川)stsyem.Accord in gly,the dyn amics of the en ergy-tra nsfer process in this systemwas an alysed. A modificatio n of the perri n formula was proposed and themecha nism of the en ergy tran sfer of t

14、he complex system was disscussed.百从I3MK和I3MK Tb (川)体系的荧光寿命、磷光寿命、量子产率，时间分辨光 谱等，计算了各主要能量传递过程的速率常数和能量传递效率，进行了该体系能量传递过程的动态分析，提出了 Perrin公式的修正参数，讨论了 I3MK Tb (川)体 系的能量传递机理.收藏指正8.The results showed that with the increasing waterstress,Fv/Fm,Fv/Fo,Yield,qP and Photo values decreased,while qN,whichreflects th

15、e protective mecha nism of light en ergy,i ncreasedsignificantly.丨研究结果表明，叶片的叶绿素荧光参数PSII原初光能转换效率(Fv/Fm)、PSII潜在 活性(Fv/Fo)、光合量子产额(Yield)、光化学淬灭系数(qP)和净光合速率(Photo)值 随水分胁迫强度增强而呈下降的趋势；收藏指正9.Either of the two stresses in creased mi nimum chlorophyllfloresce nce(F0),a nd decreased maximum chlorophyllfluoresc

16、e nee (Fm),PSn primary chemical efficiency (Fv/Fm)and PS n potential activity(Fv/F0),butdid not significantly result in variation inquantum yield of PS n linearelectron( PS ) relative to the un-stressed control. POD activity,MDA and Pro contents were sig nifica ntly raised by drought or sali ni ty.外

17、 八干旱或盐胁迫引起初始 荧光(F0)上升，最大荧光(Fm)、PS n原初光能转化 效率 (Fv/Fm)和PS n潜在活性(Fv/F0)显著下降,PS n实际光化学 量子产量(PSn )略有 降低，而POD活性、MDA和Pro含量显著提高。收藏指正10.The results showed that the diurnal variation of PS II primarily chemical efficiency (Fv/Fm), quantum yield of PS II linear electron(OPS II) and estimated electron transport

18、 rate (ETR) in 6 rice varieties related to light in ten sity.讣农舀进一步系统研究上述品种在孕穗期连体倒二叶片功能叶)的荧光参数的日变化，并解析了它们与耐光氧化和耐荫特性的关系。结果表明：6个水稻品种叶片的原初光化学效率(Fv/Fm)，实际光化学 量子产量( PSI)和通过PS n的电子传递速率(ETR)的日变化与光强有关。荧光粉及荧光量子效率检测系统The above picture shows a system for determining the quantum efficiency of phosphor powder sa

19、mples used in the lighting industry.Up to 6 samples can be loaded into the motorised carousel. A calibrated Hg source, with closed loop controller for necessary stability, is used to excite the phosphors. Significantly, the temperature of each sample can be heated to 2O0C C all under software contro

20、l.A carefully designed integrating sphere is used to collect all of the emitted light which is measured by a spectroradiometer system. Besides quantum efficiency of the phosphors and function of operating temperature, the system also provides full spectral emission and chromaticity data .荧光量子效率的测定分类:有机合成2007.9.16 00:54作者：过路庄子|评论：1 |阅读：619物质吸光跃迁至激发态后，可能会发生荧光发射、内转移，系间窜跃和外转移等过 程,也就是说,荧光发射只是多个竞争过程中的一个，因此,需要引入荧光量子效率的 概念来衡量物质荧光发射的能力.定义：荧光量子效率=发射荧光分子数/激发分子总数目前测定荧光量子效率最通用的是所谓Optically Dilute Measureme nts(TheJournalof Phyaical Chemisty,76